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2370126854B1B015035Plant Diversity as Honeybee (Apis cerana) Feed Sources Based on Identification of Pollen Characteristics in Honeycomb, in Serang PurbalinggaDesa Serang Purbalingga adalah daerah yang subur dan memiliki potensi untuk pengembangan bisnis lebah madu Apis cerana. Produk lebah madu diketahui memiliki nilai ekonomi tinggi. Pengembangan bisnis lebah madu akan lebih baik jika didukung oleh ketersediaan polen dari tumbuhan berbunga sebagai sumber pakan. Polen yang berpotensi menjadi sumber pakan A. cerana diambil dari tumbuhan bunga di sekitar sarang lebah dan dicocokkan dengan polen di dalam sarang lebah. Tujuan dari penelitian ini adalah untuk mengetahui keanekaragaman dan karakter polen dari tumbuhan yang ditemukan di sekitar sarang lebah dan di dalam sarang lebah A. cerana. Penelitian ini dilakukan dengan metode survei deskriptif dimana data yang diperoleh dari lapangan digunakan sebagai bahan untuk analisis dan menggambarkan karakteristik polen yang ditemukan. Variabel dalam penelitian ini adalah karakter polen dengan parameter adalah unit polen, ukuran, bentuk, apertura, dan ornamentasi. Berdasarkan hasil, ada 23 spesies tumbuhan yang termasuk dalam 17 famili yang ditemukan di sekitar sarang lebah dengan beragam bentuk polen, yaitu spheroidal, prolate-spheroidal, sub-prolate, dan prolate. Ukuran polen terkecil hingga terbesar adalah minutae, mediae, dan magnae. Jenis ornamentasi polen adalah rugulate, reticulate, echinate, psilate, scabrate, hingga baculate. Apertura Pollen bervariasi monosulcate, monoporate, tricolporate, tricolpate, tetracolpate, hexacolpate ke syncolpate. Karakter polen di dalam sarang identik dengan 12 polen spesies tumbuhan yang ditemukan di sekitar sarang lebah di mana bentuk polen berbentukspheroidal, prolate-spheroida, sub-prolate, dan prolate. Ada beberapa jenis ornamen, yaitu reticulate, rugulate, echinate, psilate dan sacbratte. Apertura jugabervariasi dari monosulcate, monoporate, tricolporate, tricolpate hingga syncolpate.Serang Purbalingga village is a fertile area and has the potential for the development of Apis cerana honeybee business. Honeybee products are known to have high economic value. The development of honeybee business will be better if supported by the avaibility of pollen from flowering plants as feed sources. Pollen that potential to be A. cerana feed sources are taken from plants flower around the beehive and matched with pollen inside honeycomb. The purpose of this research is to determine the diversity and character of pollen from plants found around beehive and inside A. cerana honeycomb. This research conducted by descriptive survey method where the data obtained from field used as material for analysis and describing the characteristics of pollen found. The variable in this research is pollen characters with parameters are pollen units, size, shape, apertures, and ornamentation. Based on results, there are 23 species of plants included in 17 families found around the beehive with varying of pollen shape, namely spheroidal, prolate-spheroidal, sub-prolate, and prolate. The smallest to largest pollen sizes are minutae, mediae, and magnae. Types of pollen ornamentations are rugulate, reticulate, echinate, psilate, scabrate, to baculate. Pollen apertures are varies monosulcate, monoporate, tricolporate, tricolpate, tetracolpate, hexacolpate to syncolpate. Pollen characters inside honeycomb are identical to 12 pollen of plant species found around the beehive where the pollen shape are spheroidal, prolate-spheroidal, sub-prolate and prolate. There are several types of ornamentation, namely reticulate, rugulate, echinate, psilate and sacbratte. Apertures are varies from monosulcate, monoporate, tricolporate, tricolpate to syncolpate
2370226890B1B015027Total Phenolic Content, Antioxidant Activity, and Tyrosinase Inhibitor from Marine Red Algae Extract Collected from Kupang, East Nusa TenggaraMany commercialized synthetic antioxidants are used under strict regulation in certain countries because of their potential health hazards. Thus, the search for alternative antioxidants from natural products Indonesia is needed, and one of the potential material is seaweed extract. Red seaweed is very potential to be developed as a raw material for medicines and cosmetics because it has antioxidant activity which from it’s phenolic compound that can remove the free radicals and can inhibit tyrosinase enzyme activity. The purpose of this research is to know the the total phenolic content, antioxidant activity, inhibitory activity of tyrosinase in red seaweed, and also to know the correlation between total phenolic content with antioxidant and tyrosinase inhibitor activity of red algae. The research will be conducted by quantitative methods with descriptive analysis. Parameters to be measured are the number of IC50 that obtain from DPPH, inhibition percentage of Tyrosinase analysis and the number of total phenolic compound.
This research was conduct in biotechnology lab of Ministry of Maritime Affairs and Fisheries, Jakarta. The result shows that there are 8 species of red seaweed in Kupang, East Nusa Tenggara and its consists of 4 genera. Some of species of those red seaweed are potential as antioxidant and tyrosinase inhibitors source. And the research also shows positive correlation between total phenolic content of the seaweed extract with antioxidant activity.
Many commercialized synthetic antioxidants are used under strict regulation in certain countries because of their potential health hazards. Thus, the search for alternative antioxidants from natural products Indonesia is needed, and one of the potential material is seaweed extract. Red seaweed is very potential to be developed as a raw material for medicines and cosmetics because it has antioxidant activity which from it’s phenolic compound that can remove the free radicals and can inhibit tyrosinase enzyme activity. The purpose of this research is to know the the total phenolic content, antioxidant activity, inhibitory activity of tyrosinase in red seaweed, and also to know the correlation between total phenolic content with antioxidant and tyrosinase inhibitor activity of red algae. The research will be conducted by quantitative methods with descriptive analysis. Parameters to be measured are the number of IC50 that obtain from DPPH, inhibition percentage of Tyrosinase analysis and the number of total phenolic compound.
This research was conduct in biotechnology lab of Ministry of Maritime Affairs and Fisheries, Jakarta. The result shows that there are 8 species of red seaweed in Kupang, East Nusa Tenggara and its consists of 4 genera. Some of species of those red seaweed are potential as antioxidant and tyrosinase inhibitors source. And the research also shows positive correlation between total phenolic content of the seaweed extract with antioxidant activity.
2370326848B1A015086IDENTIFIKASI GOLONGAN DARAH PADA SAMPEL DARAH KERING YANG DIPAPAR PADA SUBSTRAT KAYU, WAKTU DAN LINGKUNGAN BERBEDA Darah merupakan salah satu barang bukti kuat dalam bidang forensik untuk memecahkan suatu kasus kejahatan. Penggologan darah dengan sistem ABO digunakan dalam penyidikan untuk mendapatkan informasi mengenai identitas pemilik darah tersebut. Pengujian golongan darah akan lebih baik jika menggunakan darah segar dibandingkan dengan darah kering, namun ketersediaan darah segar cukup sulit ditemukan pada tempat kejadian perkara (TKP). Kualitas darah pada suatu TKP dapat dipengaruhi oleh substrat kayu, kondisi lingkungan, dan waktu darah itu ditemukan. Tujuan dari penelitian ini yaitu untuk mengevaluasi pengaruh substrat kayu, kondisi lingkungan, waktu dan interaksi ketiganya terhadap keberhasilan identifikasi golongan darah. Metode yang digunakan pada penelitian ini yaitu eksperimental dengan menggunakan Rancangan Acak Lengkap (RAL) dengan tiga faktor perlakuan. Faktor pertama berupa susbstrat terdiri atas kayu alam, kayu olahan, kayu dicat, dan kayu dipernis, faktor kedua berupa waktu paparan terdiri atas 72 jam, 96 jam, 120 jam dan 144 jam, faktor ketiga berupa lingkungan terdiri atas indoor dan outdoor. Setiap kombinasi perlakuan diulang 3 kali. Variabel yang diamati pada penelitian ini terdiri dari variabel bebas meliputi substrat kayu, kondisi lingkungan dan waktu; variabel terikat berupa keberhasilan identifikasi golongan darah kering. Parameter yang diamati yaitu terjadi atau tidaknya aglutinasi pada darah berdasarkan absorption-elution assay. Data disajikan dalam bentuk persentase keberhasilan dari ketiga ulangan, kemudian ditransformasi kedalam arcsine, dilanjutkan dengan uji Anova tiga arah dan data lingkungan dideskripsikan. Hasil penelitian menunjukkan sampel darah pada kondisi indoor golongan darah masih dapat diidentifikasi dengan tingkat keberhasilan 100% pada seluruh substrat kayu dan waktu pengamatan. Hasil pada kondisi outdoor golongan darah dapat diidentifikasi hingga waktu 144 jam, meskipun terjadi penurunan persentase pada 96 jam (kayu alam), 120 jam (kayu pernis), dan 144 jam (kayu dicat). Hasil analisis menunjukkan tidak adanya pengaruh signifikan dari substrat kayu, waktu, kondisi lingkungan dan interaksi ketiganya terhadap keberhasilan identifikasi golongan darah (P>0.05). Blood is one of the strong evidence in the forensics science to solve a crime case. Blood typing with ABO system is used in investigation to obtain information about the identity owner of the blood. Blood group testing would be better if using fresh blood, but fresh blood is quite difficult to find on crime scene. Quality of blood can be influenced by substrates, environmental condition, exposure time or combination of them. This experimental study was conducted using completely randomized design with three factors. The first factor was substrate namely log wood, processed wood, painted wood, and lacquered wood. The second factor was indoor and outdoor condition. The third factor was exposure time consisted of 72 hours, 96 hours, 120 hours, and 144 hours, each treatment combination was conducted in triplicates. These treatments served as independent variable, while blood agglutination as indication of successful blood group identification served as dependent variable. The observed parameter is the occurrence or absence of agglutination in blood based on absorption-elution assay. Data was presented as percentage then was analyzed using three ways Anova, while the environmental condition including air temperature, humidity and light intensity were recorded and described. Research results showed that blood samples exposed in indoor conditions can be identified 100% on all substrates and all exposure time times. While in outdoor conditions, the percentage of positively identified samples decreased to 66.67% at 96 hours for natural wood, 120 hours for lacquerware wood, and 144 hours for painted wood. Statistical analysis showed that the substrate, exposure time and environmental conditions did not significantly affect the success of blood group identification (p>0.05).
2370426849I1B015067GAMBARAN KECERDASAN INTERPERSONAL ANAK PRA SEKOLAH (4-6 TAHUN) DI TK GENUS PURWOKERTO YANG MENERAPKAN SISTEM FULLDAY SCHOOLAbstrak

GAMBARAN KECERDASAN INTERPERSONAL ANAK PRA SEKOLAH (4-6 TAHUN) DI TK GENUS DI PURWOKERTO YANG MENERAPKAN SISTEM FULLDAY SCHOOL
Rizkita Anugrah Sahara1, Dian Susmarini2, Meivita Dwi Purnamasari3

Latar belakang: Sistem fullday school tidak hanya diterapkan pada anak SD, SMP maupun SMA saja melainkan pada anak yang bersekolah di Taman kanak-kanak dengan metode pembelajaran dan pengasuhan. Pendidikan dasar yang diterimanya merupakan faktor yang dapat mempengaruhi terbentuknya kecerdasan interpersonal anak. Tujuan: Tujuan umum dari penelitian ini adalah untuk mengidentifikasi gambaran kecerdasan interpersonal anak pra sekolah yang bersekolah di TK dengan sistem fullday school. Metodologi: Penelitian ini menggunakan teknik total sampling dengan sampel sebanyak 60 responden. Pengambilan data penelitian menggunakan kuesioner. Hasil penelitian: Responden laki-laki dan perempuan memiliki jumlah sama yaitu 30 anak dengan rentang usia 4-5 tahun, usia orangtua seluruhnya berada pada rentang usia 18-40 tahun, Pendidikan orangtua mayoritas perguruan tinggi dan pekerjaan orangtua mayoritas wiraswasta dan PNS. Mayoritas anak memiliki kecerdasan interpersonal dalam kategori baik dengan skor 61-80% sebanyak 37 anak (61,7). Kesimpulan: Kecerdasan interpersonal anak di TK Genus Purwokerto menunjukkan bahwa mayoritas anak berada pada kategori baik yaitu sebanyak 37 anak (61,67%).
Abstract

DESCRIPTION OF INTERPERSONAL INTELLIGENCE OF PRE-SCHOOL CHILDREN (4 – 6 AGES) IN PURWOKERTO GENUS KINDERGARTEN APPLYING THE FULLDAY SCHOOL
Rizkita Anugrah Sahara1, Dian Susmarini2, Meivita Dwi Purnamasari3

Background: Fullday school system not only applied on elementary school, junior high school, or senior high school but also applied on kindergarten with both learning and parenting methods. A good quality of interpersonal intelligence could be seen by the ability of interaction and socialization. This basic education is factor that impact the intelligence interpersonal of child. Purpose: The general purpose of this research was to identify description of interpersonal intelligence toward pre-school children in fullday school system kindergarten. Method: This research used sampling total technique by using 60 respondents. Data were collecting by questionnaire. Result: Boys and girls respondent were 39 children, the 4 and 5 ages were same number with 30 children, parents’ age were in beginning adult range. Most of their last education were in the university and working as entrepreneur. The majority of children had good score of interpersonal intelligence with 61 – 80% score for 37 children (61,7). Conclusion: The interpersonal intelligence of Purwokerto Genus Purwokerto children showed mostly had a good condition with 37 children (61,67%).
2370526850B1B015003POLLEN MORPHOLOGY VARIATION OF UNDERGROWTH PLANTS IN BANTARBOLANG NATURE RESERVE, PEMALANG, CENTRAL JAVATumbuhan bawah merupakan tumbuhan penutup tanah yang umumnya berupa herba, rumput, semak atau perdu rendah. Sebagian besar tumbuhan bawah adalah tumbuhan berbunga. Bunga merupakan alat perkembangbiakan pada tumbuhan yang terdiri dari beberapa bagian penting seperti pedicellus, receptacullum, perianthium, pistillum dan stamen yang berisi polen. Polen merupakan bagian dari bunga yang berfungsi sebagai alat perkembangbiakan secara generatif. Polen memiliki karakter khas pada masing-masing tumbuhan sehingga dapat digunakan sebagai salah satu alat untuk mengidentifikasi tumbuhan. Pengambilan sampel penelitian dilakukan di Cagar Alam Bantarbolang yang terletak di Desa Kebon Gede, Kecamatan Bantarbolang, Kabupaten Pemalang yang banyak ditumbuhi tumbuhan liar seperti tumbuhan bawah. Penelitian ini bertujuan untuk mengetahui karakter morfologi polen tumbuhan bawah yang ada di Cagar Alam Bantarbolang. Penelitian menggunakan metode survei dengan teknik sampling menjelajah. Variabel yang diamati yaitu karakter morfologi polen dengan parameter unit polen, bentuk, apertura, diameter dan ornamentasi. Preparasi sampel menggunakan metode asetolisis dan analisis data secara deskriptif. Berdasarkan hasil penelitian ditemukan 6 spesies tumbuhan bawah yang berbeda dengan karakter morfologi polen yang berbeda pula pada bentuk polen (sub-speroidal dan prolate), apertura (tricolpate, tricolporate dan fenestrate) dan tipe ornamentasi (psilate, reticulate, perforate dan echinate). Unit polen dari semua spesies memiliki tipe yang sama yaitu monad.Undergrowth is ground cover plant which is generally in the form of herbs, grass or low shrubs. Mostly undergrowth is flowering plants. The Flower is part of plant for generative reproduction that consists of several organs that consist of pedicellus, receptacullum, perianthium, pistillum and stamen that contain pollen. Pollen is part of the flower that functions as a generative reproduction agent because it contains male gamete. Pollen has a distinctive character in each plant so that it can be used as a tool for identifying plants. Sampling location was conducted in Bantarbolang Nature Reserve, a conservation area located in Kebon Gede Village, Bantarbolang District, Pemalang Regency. The objective of research is to know the morphological character of undergrowth pollen in the Bantarbolang Nature Reserve. The research used a survey method with explorative sampling techniques. The variables that observed are the morphological character of pollen with the parameters consist of pollen unit, shape, aperture, diameter, and ornamentation. Sample preparation uses the acetolysis method and descriptive data analysis. Based on the results of the research found 6 species of undergrowth with different pollen morphological characters in the pollen shape (sub-speroidal and prolate), aperture (tricolpate, tricolporate and fenestrate) and ornamentation type (psilate, reticulate, perforate and echinate). The pollen units of all species have the same type, monad.
2370626851B1A015045Pertumbuhan dan kandungan bayam merah (Alternanthera amoena Voss) pada media tanam dengan pemberian asam humat dan ureaBayam merah (Alternanthera amoena Voss) merupakan tanaman sayur yang mengandung banyak serat. Di dalam daunnya terdapat metabolit primer berupa vitamin, mineral, serta metabolit sekunder seperti flavonoid yang berpotensi sebagai antioksidan. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh pemberian pupuk organik asam humat dan anorganik urea terhadap pertumbuhan dan kandungan flavonoid tanaman bayam merah dan mengetahui konsentrasi asam humat dan urea yang paling berpengaruh, serta interaksi keduanya dalam meningkatan pertumbuhan dan kandungan flavonoid tanaman bayam merah. Penelitian ini dilakukan secara eksperimental dengan menggunakan Rancangan Acak Lengkap (RAL) Faktorial yang terdiri atas dua faktor dengan masing-masing 4 taraf. Faktor pertama berupa pemberian asam humat dengan taraf konsentrasi yaitu 0 (kontrol); 4 g.kg-1; 8 g.kg-1; dan 12 g.kg-1. Faktor kedua berupa pemberian urea dengan taraf konsentrasi 0 (kontrol); 0,4 g.kg-1; 0,6 g.kg-1; dan 0,8 g.kg-1; masing masing perlakuan diulang sebanyak tiga kali. Variabel yang diamati dalam penelitian ini adalah pertumbuhan tanaman yang meliputi, bobot segar dan kering, serta kandungan flavonoid. Data dianalisis dengan ANOVA (Analysis of Variance), menunjukkan bahwa ada pengaruh yang signifikan pada berat segar dan kering pada pemberian pupuk urea. Analisis kemudian dilanjutkan dengan uji BNT untuk menemukan konsentrasi terbaik dari pupuk yang digunakan. Pemberian pupuk urea pada konsentrasi 4 g.kg-1 adalah konsentrasi yang paling efektif dalam meningkatkan bobot basah dan bobot kering, namun jika urea yang dikombinasikan dengan asam humat pada semua konsentrasi yang diterapkan tidak menunjukkan adanya perbedaan yang signifikan untuk semua parameter, yaitu bobot basah, bobot kering serta kandungan flavonoid.Red spinach (Alternanthera amoena Voss) is a fibery vegetable plant. The leaves contain primary metabolites such as vitamins A, B1, B2, C and niacin, as well as minerals such as Fe, Ca, Mn, and P. It also contains secondary metabolite such as flavonoids which is potentially to be used as antioxidants. The purpose of this study was to determine the effect of humic acid as organic fertilizer and urea as inorganic fertilizer on growth and flavonoid content of red spinach and determine the best concentration of humic acid and urea. The research was carried out experimentally, by using a completely randomized design (CRD) with a factorial consisting of two factors with each of the 4 levels. The first factor iss humic acid concentration that was 0 (absent); 4 g.kg-1; 8 g.kg-1; and 12 g.kg-1. The second factor was urea concentration at 0 (absent); 0.4 g.kg-1; 0.6 g.kg-1; and 0.8 g.kg-1; each treatment was performed in three replicate. The variables were observed in this study is the plant’s growth as measured in there, fresh and dry weight, and the flavonoids content. Data were analyzed by ANOVA (Analysis of Variance) test, showed that there was a significant effect on fresh and dry weight following to the application of urea fertilizer. Analyses was then continue to an LSD test to find the best concentration of fertilizer being used. Interestingly, application of urea fertilizer showly at the concentration of 4 g.kg-1 was the most effective concentration in increasing both fresh and dry weight, but however if the urea was combined with humic acid at all concentration applied did not show any significant different to all parameters; fresh, dry weight as well as flavonoid content.
2370726852B1J013119Biodegradasi Limbah Lindi Hitam, Acid Orange dan Acid Red menggunakan Jamur Trametes versicolor F200Limbah lindi hitam dan zat warna azo (acid orange dan acid red) merupakan limbah berbahaya yang dapat mengkontaminasi ekosistem, sehingga perlu dilakukan pengolahan sebelum limbah dibuang ke ekosistem. Pengolahan secara fisik maupun kimiawi memiliki kelemahan diantaranya membutuhkan input energi tinggi, biaya yang mahal, penerapan yang terbatas dan menghasilkan produk lain yang bersifat toksik. Trametes versicolor dapat dijadikan sebagai agen biodegradasi, sebab menghasilkan anzim ligninolitik seperti Lakase (Lac), Manganese peroksidase (MnP) dan Lignin peroksidase (LiP). Jamur T. versicolor F200 mampu mendekolorisasi hingga 50%pada limbah lindi hitam; 90% pada Acid orange; dan 90% pada Acid red. Aktivitas enzim Lac, MnP dan LiP terbaik masing-masing yaitu 143,59 U/L (6 hari inkubasi), 33,3 U/L (6 hari inkubasi) dan 1167,42 U/L (awal inkubasi) pada limbah lindi hitam; 520,513 U/L) (awal inkubasi), 138 U/L (awal inkubasi) dan 358 U/L (awal inkubasi) pada acid orange; dan 408,3 U/L (awal inkubasi, 77 U/L (awal inkubasi) dan 228,871 U/L (akhir inkubasi).Black liquor and azo dyes (Acid orange and acid red) are hazardous waste that can contaminate ecosystem if not treated before the waste are discharged into the ecosystem. Physical and chemical proces of these waste has a few disadvantages due to the high energy inputs, expensive cost, limited application and producing toxic byproducts. Trametes versicolor used as a biodegradation agent because it can produces ligninolytic enzyme such as Laccase (Lac), manganese Peroxidase (MnP) and Lignin Peroxidase (LiP). The aim of this study was to determine the percentage of decolorization and enzyme activity of Lac, MnP and LiP in black liquor, acid orange and acid red. The results showed decolorization occurs 50% in black liquor, 90% in acid orange and acid red. While the enzyme activity of Lac, MnP and LiP in black liquor were 143,59 U/L (6 days), 33,3 U/L (6 days) dan 1167,42 U; in acid orange were 520,513 (U/L) (0 days), 138 U/L (0 days) and 358 U/L (0 days); and in acid red were 408,3 U/L (0 days), 77 U/L (0 days) and 228,871 U/L (6 days).
2370826855I1D015039PERBEDAAN POLA MAKAN DAN PREFERENSI JENIS MAKANAN SEBELUM DAN SELAMA PUASA RAMADHAN PADA MAHASISWA OVERWEIGHT DI UNIVERSITAS JENDERAL SOEDIRMANLatar Belakang: Selama bulan Ramadhan umat islam dilarang untuk makan dan minum selama 13-14 jam perhari. Kondisi ini tentu akan mempengaruhi asupan zat gizi, frekuensi makan, serta preferensi jenis makanan.

Metodologi: Penelitian ini menggunakan rancangan one group pre-test post-test design instrumen penelitian yang digunakan adalah form food recall 1x24 jam sebelum Puasa Ramadhan dan selama Puasa Ramadhan. Data asupan zat gizi makro, dan frekuensi makan diuji normalitas dengan menggunakan uji Kolmogorov Smirnov kemudian dilanjutkan dengan uji T berpasangan untuk asupan zat gizi makro, dan Uji Wilcoxon untuk frekuensi makan.
Hasil Penelitian: Hasil penelitian menunjukkan bahwa terdapat perbedaan yang signifikan antara sebelum dan selama Puasa Ramadhan pada asupan energi dan karbohidrat dengan nilai p=0,00. Tidak terdapat perbedaan yang signifikan pada asupan protein dan lemak dengan nilai p >0,05. Terdapat perbedaan yang signifikan pada frekuensi makan antara sebelum dan selama Puasa Ramadhan dengan nilai p =0,00

Kesimpulan: Terdapat penurunan pada asupan zat gizi makro dan frekuensi makan antara sebelum dan selama Puasa Ramadhan pada mahasiswa overweight di Universitas Jenderal Soedirman.

Kata kunci: Puasa Ramadhan, Overweight, Asupan zat gizi makro.		Backgrounds: During Ramadhan, muslims are forbidden to eat and drink for 13-14 hours per day. This condition will certainly affect the intake of nutrients, frequency of food, and food type preferences.

Methods: This study uses a one group pre-test post-test design, research instrument that is used is the food recall form 1x24 hours before Ramadhan Fasting and during Ramadhan Fasting. Data on macro-nutrient intake and eating frequency were tested for normality using the Kolmogorov Smirnov Test and then followed by Paired T-Test for macro-nutrient intake, and Wilcoxon Test for eating frequency.

Results: The results showed that there were significant differences between before and during Ramadan fasting on energy and carbohydrate intake (p value = 0.00). There were no significant differences in protein and fat intake (p value > 0.05). There is a significant difference in the frequency of eating between before and during Ramadan fasting (p value = 0.00).

Conclusion: There was a decrease in macronutrient intake and frequency of eating between before and during Ramadan fasting for overweight students at Jenderal Soedirman University.

Keywords: Ramadhan fasting, Overweight, Macronutrient intake.
2370926856B1B015011Reverse Transcriptase PCR (RT-PCR) FOR DETECTION
OF DENGUE AND CHIKUNGUNYA VIRUS OF
MOSQUITO Aedes aegypti IN SOKARAJA
Aedes aegypti adalah vektor utama dari virus dengue dan chikungunya. Virus Dengue menyebabkan penyakit yang disebut Demam Dengue (DD). Sementara itu, Virus Chikungunya menyebabkan demam chikungunya. Penyakit ini melibatkan tiga organisme, diantaranya adalah virus, nyamuk Aedes, spp., dan manusia. Proses transmisi virus dengue dan chikungunya berkaitan dengan populasi dari Ae. aegypti. Kabupaten Banyumas adalah satu diantara daerah dengan banyak kasus infeksi virus dengue dan chikungunya, terutama daerah Purwokerto, Sokaraja, dan Cilongok. Hingga saat ini, tidak ad obat ataupun vaksin berlisensi yang tersedia untuk menyembuhkan virus ini secara efektif. oleh karena itu, deteksi virus pada vektor nyamuk secara efektif dapat digunakan untuk memprediksi proses transmisi virus dengue dan chikungunya. Penelitian ini bertujuan untuk megetahui deteksi molekuler virus dengue dan chikungunya pada nyamuk Ae. aegypti dewasa di wilayah Sokaraja, Kabupaten Banyumas.Survey dilakukan menggunakan metode Cross sectional di Kecamatan Sokaraja dari Mei 2019 hingga Maret 2019. Selanjutnya, teknik sampling yang digunakan adalah purposive sampling dari nyamuk Ae.aegypti menggunakan BG-Sentinal Trap, kemudian dilanjutkan dengan deteksi molekuler menggunakan RT-PCR dengan hasil bahwa Virus Dengue dan Chikungunya dari nyamuk Ae.aegypti yang dikoleksi di wilayah Sokaraja menunjukkan hasil negatif.Aedes aegypti is a primary vector of dengue and chikungunya virus. Dengue virus causes disease called dengue fever (DF). Meanwhile, chikungunya virus causes Chikungunya fever (CF). These diseases involve three organisms, namely virus, mosquito Aedes sp., and human. The transmission of dengue and chikungunya virus is related to the population of Ae. aegypti. Banyumas regency is one of the regions with many cases of dengue and chikungnya virus infections, particularly in Purwokerto, Sokaraja, and Cilongok sub-district. Up to this time, there is no medicine and vaccine provided to treat these viruses effectively. Thus, detection of virus inside vector will be effectively performed in order to predict the transmission risk of dengue and chikungunya virus. This research aimed to know the molecular detection of dengue and chikungunya virus on adult Ae.aegypti mosquito in Sokaraja Region, Banyumas Regency. Survey was done by a cross-sectional method in Sokaraja sub-district from May 2019 – March 2019. Furthermore, technical sampling that used was purposive sampling method of adult Ae.aegypti using BG-Sentital Trap, followed by molecular detection of dengue virus using Two-step RT-PCR and chikungunya gene virus using RT-PCR. Molecular detection of DENV and CHIKV of mosquitoes which collected from Sokaraja region showed negative result
2371026857B1B015002EFFECT OF BATIK WASTEWATER RESULTED FROM BIOSORPTION PROCESS TO METABOLISM RATE ON CARP (Cyprinus carpio)Penggunaan bahan kimia pada setiap proses pembuatan batik membuat limbah cair yang di hasilkannya mengandung banyak logam berat. Pembuangan limbah batik cair tanpa proses pengolahan akan mencemari lingkungan serta membahayakan kehidupan organisme sekitarnya. Logam berat adalah salah satu zat yang terdapat pada limbah batik cair. Paparan logam berat pada ikan dapat mengganggu proses metabolisme pada ikan, dan menyebabkan kematian pada ikan. Metode biosorpsi merupakan proses penyerapan logam berat dengan menggunakan organisme mati atau inaktif. Uji sub-leththal air limbah batik yang dihasilkan dari proses biosorpsi diperlukan untuk menentukan tingkat toksisitas dalam air limbah
Penelitian terkait laju metabolisme pada ikan menjadi salah satu cara untuk mengetahui pengaruh kandungan logam pada perairan. Tujuan penelitian adalah mengetahui dampak pemaparan dan mengetahui konsentrasi limbah cair batik hasil biosorpsi yang paling berdampak terhadap laju metabolisme Ikan Mas (Cyrinus carpio), terkait dengan total konsumsi oksigen, frekuensi pergerakan operculum dan glukosa darah
Penelitian dilakukan secara eksperimental menggunakan Rancangan Acak Lengkap (RAL) dengan empat perlakuan dan tiga ulangan. Perlakuan terdiri dari contratation air limbah batik yang dihasilkan dari proses biosorpsi masing-masing 0 ppm; 39.657 ppm; 26,438 ppm; dan 13.219 ppm. Variabel utama yang diamati adalah laju metabolisme ikan. Parameter utama yang digunakan adalah konsumsi oksigen total pada ikan, kadar glukosa darah, dan frekuensi gerakan operculum (OMF). Data yang diperoleh dianalisis menggunakan analisis varians ANOVA dengan tingkat kesalahan 5%. Hasilnya berbeda secara signifikan, kemudian dilanjutkan dengan uji Duncan dalam tingkat kesalahan yang sama. Hasil konsumsi oksigen pada ikan mas dengan paparan air limbah batik yang dihasilkan dari proses biosorpsi diperoleh pada 0ppm adalah 0,3453 ± 0,14076 mg / g / jam, pada 39,657ppm adalah 0,0734 ± 0,0204 mg / g / jam, pada 26,438ppm adalah 0,0810 ± 0,01960 mg / g / jam dan pada 13,219ppm adalah 0,2141 ± 0,1054 mg / g / jam. Hasil pergerakan operculum adalah K0 105 kali/menit, K1 172 kali/menit, K2 137 kali.menit, K3 112 kali/menit. Dan hasil gula darah sebesar K0 688 mg/dL, K1 132 mg/dL, K2 127 mg/dL, dan K3 116 mg/dL Berdasarkan hasil penelitian, dapat disimpulkan bahwa air limbah batik hasil biosorpsi dapat menyebabkan penurunan konsumsi oksigen atau penurunan laju metabolisme, meningkatkan pergerakan operculum dan kadar glukosa darah pada konsentrasi 39.657ppm merupakan konsentrasi yang paling berpengaruh.		The chemicals used in every process of making batik makes the batik wastewater produced contains a lot of heavy metals. Disposal unprocess of batik wastewater was pollute the environment and endanger to surrounding organisms. Heavy metal is one of the substances found in batik wastewater. Exposure to heavy metals in fish can interfere with metabolic processes in fish, and cause death in fish. The biosorption method is the process of absorbing heavy metals using dead or inactive organisms. Sub-lethal test of batik waste water produced from the biosorption process is needed to determine the level of toxicity in wastewater
Research was related to metabolic rate in fish is one way to determine the effect of metal content on water. The purpose of this study was to determine the impact of exposure and determine the concentration of batik wastewater resulted from biosorption that most affected the metabolic rate of Carp (Cyrinus carpio), related to total oxygen consumption, frequency of operculum movement and blood glucose level.
The study was conducted experimentally using a Completely Randomized Design (CRD) with four treatments and six replications. The treatment consisted of batik wastewater concentration resulted from the biosorption process of each 0 ppm; 39,657 ppm; 26,438 ppm; and 13,219 ppm. The main variable observed was the metabolic rate of fish. The main parameters was used are total oxygen consumption in fish, blood glucose levels, and frequency of operculum movement (OMF). The data obtained were analyzed using ANOVA analysis of variance with an error rate of 5%. The results were significantly different, then continued with the Duncan test in the same error rate. The results of oxygen consumption in carp with batik waste water exposure resulting from the biosorption process obtained at 0ppm is 0.3453±0.14076 mg/g/h, at 39.657ppm is 0.0734±0.0204 mg/g/h, at 26.438 ppm was 0.0810±0.01960 mg/g/hour and at 13.219ppm was 0.2141±0.1054 mg/g/h. The results of the movement of the operculum are K0 105 times/minute, K1 172 times/minute, K2 137 times/minute, K3 112 times/minute. And the results of blood sugar of K0 68 mg/dL, K1 132 mg/dL, K2 127 mg/dL, and K3 116 mg/dL. Based on the results, it can be concluded that batik waste water from biosorption was a decrease in oxygen consumption or decrease in metabolic rate, increasing the movement of operculum and blood glucose levels at a concentration of 39.657 ppm is the most influential concentration.
2371126858B1B015021Phylogenetic Relationships of Marine Ornamental Fish Members of ChaetodontidaeMarine ornamental trade has developed for several years, and Indonesia is among the biggest exporter. Various species of marine animals are traded, including ornamental fish. Chaetodontidae is among ornamental fish families sold either in local or international markets. It reported from several areas in Indonesia. However, no information was available for south coast of West Java, especially on their phylogenetic relationships. Therefore, it was unknown the phylogenetic relationships among marine ornamental fish within family Chaetodontidae from south coast of West Java. Nevertheless, that information is essential as a scientific base for management Chaetodontidae fisheries. The objectives of this research are to figure out the phylogenetic relationships among ornamental fish members of Chaetodontidae in the South Coast of West Java. Fish samples were collected purposively from two trading sites; Ujunggenteng and Taman Manalusu. Fish identification conducted according to the available reference. The evolutionary relationships among species were analyzed statistically through phylogenetic analysis based on maximum parsimony algorithm and Kimura 2- Parameter substitution model as implemented in PAUP 4.0 software. Tree branching pattern supported by 1000 bootstraps pseudo-replicates and out-group comparison. Daschyllus trimaculatus used as outgroup species. Based on the observation, Chaetodontidae from South Coast of West Java formed one monophyletic group compared to Dascylus trimaculatus with Chelmon rostratus was the basal species. All the remaining species were the derived species. There are CI and RI value gained. The consistency index (CI) value of this family is 0.5833, and the retention index (RI) is 0.5082. Marine ornamental trade has developed for several years, and Indonesia is among the biggest exporter. Various species of marine animals are traded, including ornamental fish. Chaetodontidae is among ornamental fish families sold either in local or international markets. It reported from several areas in Indonesia. However, no information was available for south coast of West Java, especially on their phylogenetic relationships. Therefore, it was unknown the phylogenetic relationships among marine ornamental fish within family Chaetodontidae from south coast of West Java. Nevertheless, that information is essential as a scientific base for management Chaetodontidae fisheries. The objectives of this research are to figure out the phylogenetic relationships among ornamental fish members of Chaetodontidae in the South Coast of West Java. Fish samples were collected purposively from two trading sites; Ujunggenteng and Taman Manalusu. Fish identification conducted according to the available reference. The evolutionary relationships among species were analyzed statistically through phylogenetic analysis based on maximum parsimony algorithm and Kimura 2- Parameter substitution model as implemented in PAUP 4.0 software. Tree branching pattern supported by 1000 bootstraps pseudo-replicates and out-group comparison. Daschyllus trimaculatus used as outgroup species. Based on the observation, Chaetodontidae from South Coast of West Java formed one monophyletic group compared to Dascylus trimaculatus with Chelmon rostratus was the basal species. All the remaining species were the derived species. There are CI and RI value gained. The consistency index (CI) value of this family is 0.5833, and the retention index (RI) is 0.5082.
2371226862B1A015067Keanekaragaman Tumbuhan Bawah pada Berbagai Umur Tegakan Pinus (Pinus merkusii) di KPH Banyumas TimurKesatuan Pemangkuan Hutan (KPH) Banyumas Timur merupakan salah satu unit pengelolaan Perum Perhutani yang mengembangkan hutan Pinus. Hutan pinus KPH Banyumas Timur terdiri dari berbagai umur. Keanekaragaman tumbuhan bawah dapat dipengaruhi oleh tutupan tajuk yang berkaitan dengan umur dari pohon di sekitar tumbuhan bawah tersebut. Tujuan dari penelitian ini yaitu untuk mengetahui keanekaragaman dan kemerataan jenis tumbuhan bawah pada berbagai umur tegakan pinus (Pinus merkusii) di KPH Banyumas Timur. Penelitian ini dilakukan di hutan pinus dengan tiga kelompok umur yang berbeda di BKPH Kebasen, KPH Banyumas Timur. Hasil penelitian menunjukan bahwa pada berbagai umur tegakan pinus di KPH Banyumas Timur terdapat 36 jenis tumbuhan bawah dari 19 familia. Berdasarkan penelitian yang telah dilakukan maka dapat diketahui bahwa keanekeragaman tumbuhan bawah pada berbagai umur tegakan pinus (Pinus merkusii) di KPH Banyumas Timur semakin tua umur tegakan pinus maka semakin sedikit jumlah jenis tumbuhan bawah. Jumlah jenis tumbuhan bawah dengan umur 12 tahun didapatkan sejumlah 20 spesies selanjutnya disusul umur 24 tahun sejumlah 18 spesies dan umur 29 sejumlah 15 spesies. Kemerataan jenis tumbuhan bawah pada berbagai umur tegakan pinus (Pinus merkusii) di KPH Banyumas Timur merata.
Forest Management Unit (KPH) East Banyumas is one of the management units of Perum Perhutani that develops pine forests. Pine forest managed by the KPH of East Banyumas consists of various ages. The diversity of undergrowth can be affects by canopy cover which is related to the age of the trees around the undergrowth. The purpose of this study is to determine the diversity and evenness of undergrowth species at various ages of pine stands (Pinus merkusii) at KPH of East Banyumas. This research was conducted in pine forests with three different age groups at the BKPH Kebasen’s, KPH of East Banyumas. The results showed that at various ages of pine stands in KPH of East Banyumas there were 36 species of undergrowth from 19 families. Based on research that has been done, it can be concluded that the diversity of undergrowth at various ages of pine stands (Pinus merkusii) in KPH of East Banyumas the older the age of pine stands, the less the number of species undergrowth. The number of undergrowth species with the age of 12 years was found 20 species, age 24 th is 18 species and age 29 th is 15 species. Evenness of the undergrowth species at various ages of pine stands (Pinus merkusii) in KPH of East Banyumas is evenly distributed, so there is no dominant species.
2371326859B1A015128Aktivitas Enzimatik Isolat Trametes spp. dari Kebun Raya Baturraden dalam Pewarna Batik Indigosol Blue dengan Variasi Konsentrasi GlukosaIndigosol Blue merupakan salah satu zat warna sintetik Antraquinon yang digunakan sebagai pewarna biru pada industri pencelupan tekstil dan bersifat rekalsitran dan non-biodegradable, sehingga tidak mudah rusak oleh perlakuan kimia maupun fotolitik. Isolat Trametes sp. diyakini memiliki kemampuan mentransformasi komponen pewarna melalui mekanisme degradasi enzimatik. Kemampuan ini karena jamur Trametes sp. mampu menghasilkan enzim ekstraseluler ligninolitik yang dapat mendegradasi komponen xenobiotik dalam limbah pewarna indigosol menjadi bentuk yang tidak toksik di lingkungan. Penelitian bertujuan untuk mengetahui kemampuan tiga isolat uji dalam menghasilkan enzim dalam pewarna Indigosol Blue pada konsentrasi glukosa berbeda serta mengetahui isolat dengan konsentrasi glukosa optimum yang memiliki aktivitas enzim terbaik dalam pewarna Indigosol Blue. Pengukuran aktivitas enzimatik dilakukan dengan metode spektrofotometri. Hasil penelitian menunjukkan bahwa isolat-isolat uji dapat menghasilkan enzim dalam pewarna Indigosol Blue pada konsentrasi glukosa berbeda. Aktivitas enzim pada masing-masing perlakuan berbeda-beda dan menunjukkan hasil yang signifikan.Indigosol Blue is one of Antraquinone synthetic dyes which is used as a blue dye in the textile industry and is recalcitrant and non-biodegradable, so it is not easily damaged by chemical or photolytic treatments. Trametes sp. isolate is believed to have the ability to transform dye components through an enzymatic degradation mechanism. This ability is due to the fungus able to produce ligninolytic extracellular enzymes that can degrade xenobiotic components in indigosol dye waste into non-toxic forms in the environment. The study aims to determine the ability of three test isolates to produce enzymes in Indigosol Blue dyes at different glucose concentrations and to determine isolate with optimum glucose concentrations that have the best enzyme activity in Indigosol Blue dyes. Measurement of enzymatic activity was carried out by the spectrophotometric method. The results showed that the test isolates could produce enzymes in Indigosol Blue dyes at different glucose concentrations. Enzyme activity in each treatment is different and shows significant results.
2371428630D1A016021Prevalensi Koksidiosis dan Identifikasi Eimeria sp. pada Kelinci Berbagai Umur di Kecamatan Kalibagor Kabupaten BanyumasPenelitian ini bertujuan untuk mengetahui prevalensi koksidiosis, mengetahui spesies Eimeria, dan mengetahui perbedaan prevalensi koksidiosis pada kelinci berbagai umur di Kecamatan Kalibagor Kabupaten Banyumas. Sasaran dari penelitian ini yaitu berbagai jenis kelinci yang terdapat di Kecamatan Kalibagor Kabupaten Banyumas pada tiga periode umur yaitu penyapihan (1-5 minggu), pertumbuhan (6-24 minggu), dan dewasa (>24 minggu) dengan jumlah kepemilikan masing-masing peternak minimal 3 ekor. Metode penelitian yang digunakan yaitu metode survei dengan menggunakan purposive sampling. Analisis data pada dilakukan dengan menggunakan metode deskriptif dan uji chi square. Sampel yang digunakan yaitu feses dari 93 ekor kelinci masing-masing sebanyak 5 gram per ekor pada periode penyapihan sebanyak 31 ekor, pertumbuhan sebanyak 31 ekor, dan dewasa sebanyak 31 ekor. Hasil analisis menunjukan bahwa spesies Eimeria yang menginfeksi pada kelinci antara lain Eimeria magna, Eimeria perforans, Eimeria flavescens, Eimeria intestinalis, Eimeria exigua, Eimeria media, Eimeria coecicola, dan Eimeria stiedae. Prevalensi koksidiosis pada kelinci periode penyapihan sebesar 29,03%, pertumbuhan sebesar 31,18%, dan dewasa sebesar 29,03% dan total prevalensi koksidiosis sebesar 89,24%. P>0,05 menunjukan bahwa tidak terdapat perbedaan prevalensi koksidiosis pada kelinci berbagai umur di Kecamatan Kalibagor Kabupaten Banyumas. Kesimpulan dari penelitian ini bahwa prevalensi koksidiosis tidak berbeda nyata pada kelinci periode penyapihan, pertumbuhan, dan dewasa.The purpose of this study are to determine the prevalence of coccidiosis, to know the species of Eimeria, and to find out the difference in the prevalence of coccidiosis in various age rabbits in Kalibagor Subdistrict Banyumas Regency. The targets of this study is various types of rabbits in Kalibagor Subdistrict Banyumas Regency at three periods, namely weaning (1-5 weeks), growing (6-24 weeks), and adult (>24 weeks) with a minimum 3 rabbit of each farmer. The method used was a survey method using purposive sampling. Data analysis was performed using descriptive and chi square methods. The samples used for this study were rabbit's faeces as much as 5 grams per animal in the weaning period as many as 31 , 31 growing, and 31 adult. The results of the analysis show that the species of Eimeria identified as infecting rabbits includes Eimeria magna, Eimeria perforans, Eimeria flavescens, Eimeria intestinalis, Eimeria exigua, Eimeria media, Eimeria coecicola, and Eimeria stiedae. The prevalence of coccidiosis in rabbits weaning period was 29,03%, the growing was 31,18%, and the adult was 29,03% and the total prevalence of coccidiosis were 89,24%. P>0,05 shows that there are no difference in the prevalence of coccidiosis in rabbits of various ages in Kalibagor Subdistrict Banyumas Regency. The conlusion of this study is that the prevalence of coccidiosis was noy significantly different in rabbits in weaning, growing, and adult period.
2371528944D1A016029Dampak Wabah Virus Corona (Covid-19) Terhadap Kegiatan Ekonomi Usaha Peternakan Ayam Broiler Kemitraan di Kabupaten Banyumas
(The Impact of Corona Virus Outbreak (Covid-19) on the Economic Activities of Partnership Broiler Chicken Farming in Banyumas Regency)
Penelitian Dampak Wabah Virus Corona (Covid-19) Terhadap Kegiatan Ekonomi Usaha Peternakan Ayam Broiler Kemitraan di Kabupaten Banyumas dilaksanakan pada tanggal 30 April 2020 sampai dengan tanggal 14 Mei 2020 di Kabupaten Banyumas. Wabah virus corona tidak hanya menimbulkan dampak ekonomi secara umum namun juga berdampak pada kegiatan ekonomi usaha peternakan ayam broiler kemitraan khususnya di Kabupaten Banyumas. Penelitian ini bertujuan untuk mengetahui profil peternak ayam broiler kemitraan di Kabupaten Banyumas dan mengetahui kegiatan ekonomi usaha peternakan ayam broiler kemitraan di Kabupaten Banyumas sebelum dan selama adanya wabah virus corona yang meliputi kemudahan dalam menjual ternak ayam broiler, harga jual, dan memperoleh keuntungan usaha.
Penelitian dilakukan dengan metode survey dengan teknik penetapan sampel menggunakan accidental sampling dengan kriteria peternak sudah melakukan usaha peternakan ayam broiler kemitraan minimal satu tahun. Peternak yang akan dijadikan responden minimal berjumlah 30 responden. Kuisioner yang diberikan secara online menggunakan google formulir. Analisis yang digunakan yaitu deskriptif dengan pengukuran modus atau nilai yang paling sering muncul.
Hasil penelitian menunjukkan bahwa peternak ayam broiler kemitraan di Kabupaten Banyumas rata-rata sudah beternak lebih dari 10 tahun, namun dengan skala populasi yang masih relatif kecil yaitu 1.000-5.000 ekor dengan sebagian besar peternak memiliki pendidikan terakhir SMA dan menjadikan usaha peternakan menjadi pekerjaan utama. Sebelum wabah virus corona peternak merasa mudah dalam menjual produk hasil usaha, harga jual produk bagus dan dalam memperoleh keuntungan usaha mudah. Sedangkan selama wabah virus corona peternak merasa sulit dalam menjual produk hasil usaha, harga jual lebih rendah dan dalam memperoleh keuntungan sulit. Kegiatan ekonomi usaha peternakan ayam broiler kemitraan selama wabah virus corona yang berdampak langsung terhadap peternak yaitu kemudahan dalam menjual dan kemudahan dalam memperoleh keuntungan usaha.		Research on the Impact of Corona Virus Outbreak (Covid-19) on the Economic Activities of Partnership Broiler Chicken Farming in Banyumas Regency was conducted on April 30th, 2020 until May 14th, 2020 in Banyumas Regency. The corona virus outbreak not only caused a general economic impact but also affected the economic activities of the partnership broiler chicken farming business, especially in Banyumas Regency. This study aims to determine the profile of partnership broiler breeders in the Banyumas Regency and to know the economic activities of partnership broiler chicken farms in Banyumas Regency before and during the corona virus outbreak which includes the ease of selling broiler chickens, selling prices, and getting business profits.
This research was conducted by the survey method with the technique of determining samples using accidental sampling, with criteria to have conducted a broiler chicken farming partnership for a minimum of one year. The number of respondents is at least 30 farmers. Questionnaires were given online using google forms. The analysis used is descriptive with the measurement mode or value that appears most often.
The results of the research showed that the partnership broiler breeders in Banyumas Regency have been raising chicken for over 10 years on average, but with a relatively small population scale of 1,000-5,000 with most farmers having high school education and making livestock business as the major occupation. Before the corona virus outbreak, farmers found it easy to sell their products, got good selling prices, and felt easy to get business profits, while during the corona virus outbreak, farmers found it difficult to sell their products, got a lower selling price, and felt difficult to get business profits. The economic activities of the partnership broiler chicken farming business during the corona virus outbreak have a direct impact on farmers, namely in the ease of selling and of obtaining business profits.
2371626860B1A015083KEANEKARAGAMAN TUMBUHAN PAKU TERESTRIAL DI CAGAR ALAM BANTARBOLANG PEMALANG
JAWA TENGAH
Tumbuhan paku adalah tumbuhan perintis yang dapat ditemukan di setiap tipe kawasan hutan dan memegang peranan penting dalam menyusun ekosistem hutan. Kawasan hutan dapat mengalami perubahan fungsi yang antara lain diakibatkan oleh perbedaan kondisi di tepi hutan dengan di dalam hutan. Dampak dari bertemunya dua kondisi lingkungan yang berbeda tersebut terhadap tumbuhan dan hewan dapat disebut efek tepi (edge effect). Penelitian dilakukan di Cagar Alam Bantarbolang Pemalang, Jawa Tengah. Tujuan dari penelitian ini untuk mengetahui keanekaragaman dan faktor lingkungan tumbuhan paku terestrial di Cagar Alam Bantarbolang. Data yang diperoleh dianalisis menggunakan Indeks Nilai Penting (INP), Indeks Keanekaragamaan (H’) Shannon-Wiener, Indeks Kemerataan Spesies (e), dan Indeks Kesamaan Komunitas (IS).
Hasil penelitian di Cagar Alam Bantarbolang diperoleh tumbuhan paku terestrial sebanyak 10 spesies yang terdiri dari 400 individu termasuk dalam 6 familia. Spesies yang paling banyak ditemukan yaitu Stenochlaena palustris dengan 205 individu. Cagar Alam Bantarbolang dipengaruhi oleh efek tepi, karena semakin ke dalam hutan jumlah spesies tumbuhan paku terestrial semakin sedikit. Faktor lingkungan yang berpengaruh terhadap jumlah spesies tumbuhan paku terestrial yaitu suhu, intensitas cahaya dan pH tanah.

Kata kunci: Cagar Alam Bantarbolang, efek tepi, keanekaragaman, pteridophyta, terestrial.
Ferns are pioneering plants that can be found in every type of forest areas and have important role in forest ecosystems. Forest areas may be changed functionally which are caused by condition differences between the edge of the forest and inside the forest. The collision of these two different environmental conditions which affects the plants and animals called edge effect. The study was conducted at the Bantarbolang Pemalang Nature Reserve, Central Java. The aim of this study is to determine the diversity and environmental factors of terrestrial ferns in the Bantarbolang Nature Reserve. The obtained data were analyzed using the Importance Value Index (IVI), Shannon-Wiener Diversity Index (H'), Species Evenness Index (e), and Community Similarity Index (IS).
The result of research in the Bantarbolang Nature Reserve is 10 terrestrial fern plant species were found, in which consisted of 400 individuals including 6 families. The most commonly found species was Stenochlaena palustris with 205 individuals. Bantarbolang Nature Reserve was influenced by edge effects, because the number of terrestrial fern plant species was getting lower inside the forest. Environmental factors that influenced the number of terrestrial fern species are temperature, light intensity, and soil pH.

Keywords: Bantarbolang Nature Reserve, diversity, edge effects, pteridophyta, terrestrial.
2371726881I1E015001Pengaruh Metode Latihan Drill Konstan Terhadap Ketepatan Forehand Drive Siswa Ekstrakurikuler Tenis Meja Di SMP N 1 BaturadenAbstrak
PENGARUH METODE LATIHAN DRILL KONSTAN TERHADAP KETEPATAN FOREHAND DRIVE
SISWA EKSTRAKURIKULER TENIS MEJA
DI SMP N 1 BATURADEN
Anggit Eka Wahyuni
Latar Belakang:Tenis meja adalah salah satu permainan bola kecil yang dimainkan dengan menggunakan bet dan bola tenis meja yang terbuat dari celluloid. Penelitian ini bertujuan untuk mengetahui pengaruh model latihan drill konstan terhadap ketepatan forehand drive siswa ekstrakurikuler tenis meja di SMP N 1 Baturaden.
Metodologi: Penelitian ini merupakan penelitian pre-Eksperimental Design dengan one group pretest-posttest design. Sample dari penelitian ini diambil menggunakan total sampling dengan jumlah 13 siswa. Instrumen tes menggunakan ketepatan forehand drive yaitu melakukan rally selama 30 detik ke arah sasaran dan dilakukan 2 kali percobaan. Analisis data yang digunakan adalah Uji T (Paired Samples Test).
Hasil Penelitian: Hasil penelitian diketahui bahwa: (1) Terdapat pengaruh metode latihan drill konstan terhadap ketepatan forehand drive dengan nilai Sig (p=0,000). (2)Metode latihan drill konstan dapat mempengaruhi ketepatan forehand drive dengan rata-rata peningkatan setiap siswa sebesar 41,25%.
Kesimpulan: Terdapat pengaruh metode latihan drill konstan terhadap ketepatan forehand drive siswa ekstrakurikuler tenis meja di SMP N 1 Baturaden.
Kata Kunci: Tenis Meja, Forehand, Drill, Ketepatan		Abstract
THE EFFECT OF EXERCISE MODEL BY DRILL CONSTANT
ON THE ACCURACY OF FOREHAND DRIVE
AT EXTRACURRICULAR TABLE TENNIS
OF SMP N 1 BATURADEN
Anggit Eka Wahyuni
Background: Table tennis is one of the small ball games that is played using bet and table tennis balls made of celluloid. This study aims to determine the effect of a constaint drill training model on the accuracy of the forehand drive extracurricular table tennis student at SMP N 1 Baturaden.
Methodology: This research is a pre-experimental design research with one group pretest-posttest design. Samples from this study were taken using total sampling with a total of 13 students. The test instrument uses the accuracy of the forehand drive that is to rally for 30 seconds towards the target and conducted 2 trials. Analysis of the data used is the T Test (Paired Samples Test).
Research Results: The results of the study note that: (1) There is an effect of the constant drill training method on the accuracy of the forehand drive with a Sig (p = 0,000). (2) The method of constant drill practice can affect the accuracy of the forehand drive with an average increase of each student by 41.25%.
Conclusion: There is an effect of the constant drill training method on the accuracy of the forehand drive of table tennis extracurricular students at SMP N 1 Baturaden.
Keywords: Table Tennis, Forehand, Drill, Accuracy
2371826863I1B015071GAMBARAN PENGETAHUAN DAN SIKAP IBU HAMIL TRIMESTER III TERKAIT PEMBERIAN MP-ASI DINIGAMBARAN PENGETAHUAN DAN SIKAP IBU HAMIL TRIMESTER III TERKAIT PEMBERIAN MP-ASI DINI
Iftiar Alif Nuraeni¹, Dian Susmarini², Meivita Dewi Purnamasari3

1Mahasiswa Jurusan Keperawatan, Fakultas Ilmu-Ilmu Kesehatan, Universitas Jenderal Soedirman
2,3Dosen Jurusan Keperawatan, Fakultas Ilmu-Ilmu Kesehatan, Universitas Jenderal Soedirman
Latar belakang: Makanan pendamping ASI (MP-ASI) adalah makanan atau minuman tambahan yang mengandung zat gizi dan diberikan kepada bayi usia 6-24 bulan untuk memenuhi kebutuhan gizi selain dari ASI. Pemberian MP-ASI terlalu dini bergantung sepenuhnya pada pengetahuan dan sikap ibu merupakan salah satu faktor yang mempengaruhi ibu dalam pemberian makanan pendamping ASI.
Tujuan: Mengetahui gambaran pengetahuan dan sikap ibu hamil trimester III terkait pemberian MP-ASI dini.
Metodologi: penelitian ini menggunakan metode deskriptif kuantitatif dengan desain cross sectional. Sampel berjumlah 46 responden yang memenuhi kriteria inklusi dan kriteria ekslusi. Pengetahuan dan sikap diukur menggunakan kuesioner Hajrah. Data dianalisi menggunakan uji univariat.
Hasil: Sebagian besar responden mengalami pengetahuan yang baik (78,7%) dan memiliki sikap yang sangat baik (87,2%). Pengetahuan baik cenderung dialami oleh usia 26-35 tahun, berpendidikan SMA, ibu rumah tangga dan ibu multigravida. Sedangkan sikap sangat baik dialami oleh usia 26-35 tahu, berpendidikan SMP, ibu rumah tangga dan ibu primigravida.
Kesimpulan: Ibu hamil trimester III cenderung memiliki pengetahuan dan sikap yang sangat baik.

Kata kunci: Makanan pendamping ASI, pengetahuan, sikap, kehamilan.


¹Mahasiswa Jurusan Keperawatan, Fakultas Ilmu-ilmu Kesehatan, Universitas
Jenderal Soedirman.
2,1 Departemen Keperawatan Anak, Fakultas Ilmu-ilmu Kesehatan, Universitas Jenderal Soedirman.

DESCRIPTION OF KNOWLEDGE AND ATTITUDE OF TRIMESTER III PREGNANT WOMEN RELATED TO THE PROVISION OF EARLY MP-ASI
Iftiar Alif Nuraeni¹, Dian Susmarini², Meivita Dewi Purnamasari3

1Mahasiswa Jurusan Keperawatan, Fakultas Ilmu-Ilmu Kesehatan, Universitas Jenderal Soedirman
2,3Dosen Jurusan Keperawatan, Fakultas Ilmu-Ilmu Kesehatan, Universitas Jenderal Soedirman
Abstract

Background: ASI complementary foods (MP-ASI) are supplementary foods or drinks that contain nutrients and are given to babies aged 6-24 months to meet nutritional needs other than breast milk. Giving MP-ASI too early depends entirely on the knowledge and attitude of the mother is one of the factors that influence the mother in providing complementary feeding.
Objective: To know the description of knowledge and attitudes of third trimester pregnant women related to early breastfeeding MP.
Methodology: this study uses quantitative descriptive methods with cross sectional design. The sample consisted of 46 respondents who met the inclusion and exclusion criteria. Knowledge and attitude are measured using the Hajrah questionnaire. Data were analyzed using univariate tests.
Results: Most respondents experienced good knowledge (78.7%) and had a very good attitude (87.2%). Good knowledge tends to be experienced by people aged 26-35 years old, high school educated, housewives and multigravida mothers. While a very good attitude is experienced by people aged 26-35, middle school educated, housewives and primigravida mothers.
Conclusion: Third trimester pregnant women tend to have very good knowledge and attitude.
Keywords: complementary feeding, knowledge, attitude, pregnancy.
2371926864I1D015008PENGARUH PEMBERIAN YOSAKAME (YOGURT SUSU KECAMBAH KACANG MERAH) TERHADAP KADAR TRIGLISERIDA DAN LDL PADA PENDERITA DISLIPIDEMIAABSTRAK
PENGARUH PEMBERIAN YOSAKAME (YOGURT SUSU KECAMBAH KACANG MERAH) TERHADAP KADAR TRIGLISERIDA DAN LDL (LOW DENSITY LIPOPROTEIN) PADA PENDERITA DISLIPIDEMIA
Arinda Dyah P, Hery W, Aisyah Tri S

Latar Belakang: Dislipidemia adalah salah satu faktor resiko terjadinya penyakit kardiovaskular pada lansia. Yosakame memiliki kandungan senyawa fenolik dan serat larut air yang diyakini mampu menurunkan kadar trigliserida dan LDL. Penelitian ini bertujuan untuk mengetahui pengaruh produk yosakame terhadap kadar trigliserida dan LDL plasma pada penderita dislipidemia.
Metodologi: Penelitian ini merupakan penelitian true eksperimental menggunakan rancangan prepost test control group design.Penelitian ini menggunakan responden wanita lansia usia 45 – 80 tahun, memiliki IMT > 25 kg/m2 , Trigliserida > 200 mg/dL dan kasar LDL > 150 mg/dL di Posyandu Lansia Bina Karahayon sebanyak 18 orang yang dibagi 2 kelompok yaitu kelompok yosakame dan kelompok plasebo, masing – maring terdiri dari 9 orang. Intervensi produk yosakame sebesar 200 ml/hari dilakukan selama 2 bulan.Pengambilan sampel darah dilakukan pada bulan ke-0 sebelum intervensi dan bulan ke-2 sesudah intervensi. Analisis Pengaruh pemberian yosakame terhadap kadar trigliserida menggunakan uji Wilcoxon dan Mann Whitney, untuk kadar LDL menggunakan uji Paired T-test dan Independent T-test.
Hasil Penelitian:Responden penelitian bersifat homogen (p>0,05), terjadi penurunan kadar trigliserida sebesar 22,16% kelompok plasebo dan 19,74% pada kelompok yosakame (p>0,05). Pada uji kadar LDL terjadi penurunan pada kelompok yosakame dibandingkan dengan kelompok plasebo (p<0,05).
Kesimpulan:Yosakame dengan dosis 200 ml/hari selama 2 bulan tidak dapat berpengaruh pada kadar trigliserida penderita dislipidemia, sedangkan pemberian yosakame berpengaruh signifikan terhadap kadar LDL penderita dislipidemia.
The Effect of Yosakame (Milk Yogurt of Red Bean Sprouts) on Triglyceride and LDL Levels in Dyslipidemia Patients
Arinda Dyah Putri1, Hery Winarsi2, Aisyah Tri Septiana3
Background: Dyslipidemia is one of risk factors for cardiovascular disease in the elderly. Yosakame contains phenolic compounds and water soluble fibers which are believed to be able to reduce triglyceride and LDL levels. This study aims to determine the effect of yosakame products on triglyceride and plasma LDL levels in dyslipidemia patients.
Methodology: This study is a true experimental study using a plan of prepost test control group design. This study used elderly female respondents aged 45-80 years, had an IMT > 25 kg/m2, triglyceride > 200 mg/dL and LDL> 150 mg/dL in Posyandu Lansia Bina Karahayon as many as 18 people divided into 2 groups namely yosakame group and placebo group, each consisting of 9 people. Intervention of yosakame products of 200 ml/day was carried out for 2 months. Blood samples were taken at 0th month before the intervention and 2nd month after the intervention. Analysis of the effect of awarding yosakame to triglyceride levels using the Wilcoxon and Mann Whitney tests, for LDL levels using the Paired T-test and Independent T-test.
Research Result:Respondents in this study were homogenous (p>0,05). Triglycerides levels were decrease on 22,16% for placebo’s group and 19,74% for yosakame’s group. LDL levels were decrease for yosakame’s group and increase for placebo’s group (p<0,05).
Conclusion:Yosakame with a doseage of 200 ml / day for 2 months can not affect the triglyceride levels of patients with dyslipidemia, while the awarding of yosakame has a significant effect on LDL levels of patients with dyslipidemia.
Keywords: red bean yogurt, dyslipidemia, LDL, triglyceride
2372026865I1D015006Ade Uswatun Hasanah I1D015006 pada Remaja Status Gizi Normal dan Lebih di SMA Negeri 1 SokarajaPAPARAN SCREEN TIME DAN TINGKAT ASUPAN GIZI MAKRO PADA REMAJA STATUS GIZI NORMAL DAN LEBIH
DI SMA NEGERI 1 SOKARAJA
Ade Uswatun Hasanah, Yovita Puri Subardjo, Izka Sofiyya Wahyurin

Latar Belakang : Prevalensi gizi lebih pada remaja tahun 2013-2018 meningkat di Indonesia. Salah satu faktor yang mempengaruhi diantaranya adalah iklan dan asupan makan. Penelitian ini bertujuan untuk mengetahui perbedaan paparan screen time dan tingkat asupan gizi makro antara remaja gizi normal dan lebih di SMA Negeri 1 Sokaraja.
Metodologi : Desain penelitian yang digunakan yaitu case control dengan sampel 35 responden pada tiap kelompok. Pengambilan data screen time diambil dengan kuesioner The HELENA Study, tingkat asupan makro diambil dengan SQ-FFQ dan status gizi ditentukan oleh indeks IMT/U. Analisis data menggunakan Independent-Samples T Test dan Mann Whitney.
Hasil : Terdapat perbedaan paparan screen time p=0,003 dan tingkat asupan gizi protein p=0,005, lemak p=0,014 dan karbohidrat p=0,000 pada remaja gizi normal dan lebih di SMA Negeri 1 Sokaraja.
Kesimpulan : Terdapat perbedaan paparan screen time dan tingkat asupan gizi makro pada remaja gizi normal dan lebih di SMA Negeri 1 Sokaraja.
Kata Kunci : Screen time, tingkat asupan gizi makro, status gizi lebih		SCREEN TIME EXPOSURE, MACRO-NUTRIENT INTAKE LEVEL AND NUTRITION STATUS OF THE TEENAGERS IN SOKARAJA 1 SENIOR HIGH SCHOOL
Ade Uswatun Hasanah, Yovita Puri Subardjo, Izka Sofiyya Wahyurin

Background: The prevalence of excessive nutrition of adolescents in 2013-2018 is increasing in Indonesia. One of the influencing factors is advertising and food intake. The purpose of this research is to found out whether there is rhe difference of screen time exposure and macro-nutrient intake level of those teenagers who have normal, overweight and obesity nutrition status in Sokaraja 1 Senior High School.
Method: This research used case control design within 35 people as sample in every group. Screen time data were taken by The HELENA Study questionnaire, level of macro nutrient intake were taken with SQ-FFQ, and nutrition status were determined by IMT/U. The data analyzed by Independent-Samples T Test and Mann Whitney.
Result: There were differences on screen time exposure p=0,003 and level of nutrition intake of protein p=0,005, fat p=0,014 and carbohydrate p=0,000 of the teenagers who were having normal, overweight and obesity nutritional status in Sokaraja 1 Senior High School.
Conclusion: There were differences on screen time exposure and macro-nutrient intake level of those teenagers who have normal, overweight and obesity nutrition status in Sokaraja 1 Senior High School.
Keywords: Macro-nutrient intake level, nutritional status, screen time