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Total Phenolic Content, Antioxidant Activity, and Tyrosinase Inhibitor from Marine Red Algae Extract Collected from Kupang, East Nusa Tenggara
Abstrak (Bhs. Indonesia)
Many commercialized synthetic antioxidants are used under strict regulation in certain countries because of their potential health hazards. Thus, the search for alternative antioxidants from natural products Indonesia is needed, and one of the potential material is seaweed extract. Red seaweed is very potential to be developed as a raw material for medicines and cosmetics because it has antioxidant activity which from it’s phenolic compound that can remove the free radicals and can inhibit tyrosinase enzyme activity. The purpose of this research is to know the the total phenolic content, antioxidant activity, inhibitory activity of tyrosinase in red seaweed, and also to know the correlation between total phenolic content with antioxidant and tyrosinase inhibitor activity of red algae. The research will be conducted by quantitative methods with descriptive analysis. Parameters to be measured are the number of IC50 that obtain from DPPH, inhibition percentage of Tyrosinase analysis and the number of total phenolic compound. This research was conduct in biotechnology lab of Ministry of Maritime Affairs and Fisheries, Jakarta. The result shows that there are 8 species of red seaweed in Kupang, East Nusa Tenggara and its consists of 4 genera. Some of species of those red seaweed are potential as antioxidant and tyrosinase inhibitors source. And the research also shows positive correlation between total phenolic content of the seaweed extract with antioxidant activity.
Abtrak (Bhs. Inggris)
Many commercialized synthetic antioxidants are used under strict regulation in certain countries because of their potential health hazards. Thus, the search for alternative antioxidants from natural products Indonesia is needed, and one of the potential material is seaweed extract. Red seaweed is very potential to be developed as a raw material for medicines and cosmetics because it has antioxidant activity which from it’s phenolic compound that can remove the free radicals and can inhibit tyrosinase enzyme activity. The purpose of this research is to know the the total phenolic content, antioxidant activity, inhibitory activity of tyrosinase in red seaweed, and also to know the correlation between total phenolic content with antioxidant and tyrosinase inhibitor activity of red algae. The research will be conducted by quantitative methods with descriptive analysis. Parameters to be measured are the number of IC50 that obtain from DPPH, inhibition percentage of Tyrosinase analysis and the number of total phenolic compound. This research was conduct in biotechnology lab of Ministry of Maritime Affairs and Fisheries, Jakarta. The result shows that there are 8 species of red seaweed in Kupang, East Nusa Tenggara and its consists of 4 genera. Some of species of those red seaweed are potential as antioxidant and tyrosinase inhibitors source. And the research also shows positive correlation between total phenolic content of the seaweed extract with antioxidant activity.
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