Update Artikelilmiah: 54801

MUTHIA DWI MAULIDINAH

NIM

Judul Artikel

Abstrak (Bhs. Indonesia)

Aeromonas hydrophila merupakan bakteri patogen penyebab Motile Aeromonas 
Septicemia (MAS) pada ikan air tawar yang pengendaliannya masih banyak 
menggunakan antibiotik. Penggunaan antibiotik secara berlebihan berpotensi 
menyebabkan resistensi bakteri sehingga diperlukan alternatif antibakteri alami, 
salah satunya eco-enzyme. Penelitian ini bertujuan mengetahui kemampuan ecoenzyme berbahan dasar campuran limbah buah jeruk dan buah pepaya dalam 
menghambat pertumbuhan A. hydrophila serta mengetahui konsentrasi yang 
memberikan daya hambat terhadap bakteri tersebut. Penelitian menggunakan 
Rancangan Acak Lengkap (RAL) dengan empat konsentrasi eco-enzyme, yaitu 
25%, 50%, 75%, dan 100%, kontrol positif amoxicillin, serta kontrol negatif 
akuades asam, masing-masing tiga ulangan. Parameter yang diamati meliputi 
karakteristik eco-enzyme, kandungan senyawa aktif, dan aktivitas antibakteri 
menggunakan metode difusi sumuran. Data dianalisis menggunakan uji 
Kruskal–Wallis yang dilanjutkan dengan uji Dunn–Bonferroni. Eco-enzyme
memiliki karakteristik suhu 25–30°C, pH 2,9–6,5, dan TDS 2.450–4.450 ppm serta 
mengandung tanin, flavonoid, dan steroid. Zona hambat terbentuk pada 
konsentrasi 50%, 75%, dan 100% dengan diameter berturut-turut 3,50 ± 0,50 mm, 
5,33 ± 0,577 mm, dan 6,75 ± 1,392 mm. Hasil uji Kruskal–Wallis menunjukkan 
perbedaan signifikan (p = 0,038), sedangkan uji Dunn–Bonferroni menunjukkan 
perbedaan nyata antara konsentrasi 50% dan 100% (p = 0,033). Eco-enzyme
campuran limbah jeruk dan pepaya memiliki aktivitas antibakteri terhadap A. 
hydrophila, tetapi belum dapat ditetapkan satu konsentrasi sebagai konsentrasi
optimal berdasarkan hasil uji statistik.

Abtrak (Bhs. Inggris)

Aeromonas hydrophila is a pathogenic bacterium that causes Motile Aeromonas 
Septicemia (MAS) in freshwater fish, which is commonly controlled using antibiotics. 
Excessive use of antibiotics may lead to bacterial resistance, creating a need for natural 
antibacterial alternatives, such as eco-enzyme. This study aimed to determine the ability 
of eco-enzyme produced from mixed orange and papaya fruit waste to inhibit the growth 
of A. hydrophila and to determine the concentrations that exhibited antibacterial activity 
against the bacterium. A Completely Randomized Design (CRD) was applied using four 
eco-enzyme concentrations, namely 25%, 50%, 75%, and 100%, a positive control of 
amoxicillin, and a negative control of acidified distilled water, with three replications 
each. The observed parameters included eco-enzyme characteristics, active compounds, 
and antibacterial activity using the agar well diffusion method. Data were analyzed using 
the Kruskal–Wallis test followed by the Dunn–Bonferroni test. The eco-enzyme had 
temperatures of 25–30°C, pH values of 2.9–6.5, and Total Dissolved Solids (TDS) of 
2,450–4,450 ppm, and contained tannins, flavonoids, and steroids. Inhibition zones were 
observed at concentrations of 50%, 75%, and 100%, with diameters of 3.50 ± 0.50 mm, 
5.33 ± 0.577 mm, and 6.75 ± 1.392 mm, respectively. The Kruskal–Wallis test showed a 
significant difference (p = 0.038), while the Dunn–Bonferroni test showed a significant 
difference between the 50% and 100% concentrations (p = 0.033). Eco-enzyme produced 
from mixed orange and papaya fruit waste exhibited antibacterial activity against A. 
hydrophila, but a single optimal concentration could not be determined based on the 
statistical analysis.

Kata kunci

Pembimbing 1

Pembimbing 2

Pembimbing 3

Tahun

Jumlah Halaman