Update Artikelilmiah: 36104

ARY KURNIA ADHI

NIM

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Abstrak (Bhs. Indonesia)

Minyak atsiri sereh wangi (Cymbopogon nardus L. Rendle) mengandung 
metabolit sekunder yang bermanfaat. Senyawa yang terdapat dalam minyak sereh 
tersebut merupakan zat aktif yang berpotensi sebagai antimikroba. Tujuan dari 
penelitian ini yaitu membuat formulasi nanoemulsi minyak sereh wangi, menentukan 
karakteristik nanoemulsi minyak sereh wangi, dan menentukan aktivitas antimikroba 
nanoemulsi minyak sereh wangi dengan metode sumuran. Pembuatan formulasi 
nanoemulsi dilakukan dengan metode energi rendah. Formulasi nanoemulsi dibuat 
dengan 3 variasi konsentrasi minyak yaitu F1 (2%), F2 (4%), dan F3 (6%), selain itu 
dibuat juga formulasi emulsi dan minyak sereh wangi yang diencerkan dengan 
konsentrasi yang serupa dengan nanoemulsi. Surfaktan dan kosurfaktan yang digunakan 
untuk membuat nanoemulsi yaitu tween 80 dan propilen glikol dengan perbandingan 
20:15. Karakterisasi sediaan nanoemulsi meliputi pengukuran distribusi partikel, uji 
organoleptis, pengukuran persen transmittan, pengukuran viskositas, pengukuran pH, 
pemeriksaan tipe nanoemulsi, uji freeze-thaw cycle, dan uji sentrifugasi. Ukuran partikel 
semua formulasi nanoemulsi minyak sereh wangi konsentrasi 2% sebesar 17,21 
nm(100%) 4% sebesar 22,89 nm(100%), dan 6% sebesar 77,60 nm(15,5%) dan 16,43 
nm(84,5%). Hasil karakterisasi uji organoleptis sediaan nanoemulsi menunjukkan 
semakin besar konsentrasi minyak, warna akan semakin kuning, bau semakin wangi, 
dan kejernihan berkurang. Nilai persen transmitan nanoemulsi minyak sereh wangi 
konsentrasi 2, 4, dan 6% berturut-turut yaitu 99,230±0,058; 98,030±0,058; dan 
97,500±0,000% sedangkan emulsi dengan konsentrasi serupa yaitu 99,100±0,000; 
71,300±0,000; dan 54,530±0,058%. Nilai viskositas nanoemulsi minyak sereh wangi 
konsentrasi 2, 4, dan 6% berturut-turut 6,0924; 11,3418; dan 16,1668 cP. Tipe 
nanoemulsi yang dihasilkan adalah tipe O/W. Nilai pH nanoemulsi minyak sereh wangi 
konsentrasi 2, 4, dan 6% berturut-turut yaitu 5,913±0,011; 5,906±0,006; dan 
5,873±0,012. Hasil uji sentrifugasi dan freeze-thaw cycle nanoemulsi minyak sereh 
wangi menunjukkan tingkat kestabilan fisik yang baik, Secara umum karakteristik dan 
kestabilan nanoemulsi lebih baik dibandingkan dengan emulsi. Hasil uji sentrifugasi 
emulsi dan nanoemulsi minyak sereh wangi tidak menunjukkan pengendapan ataupun 
pemisahan fasa. Zona hambat antibakteri P. acne nanoemulsi 2, 4, 6% berturut-turut 
yaitu 1,530±0,742; 2,500±0,354; dan 6,180±1,450 mm. Zona hambat antijamur C. 
albicans nanoemulsi 2, 4, 6% berturut-turut yaitu 0,000±0,000; 2,750±0,354; dan 
6,050±0,071 mm

Abtrak (Bhs. Inggris)

Citronella (Cymbopogon nardus L. Rendle) essential oil contains beneficial 
secondary metabolites. The compounds contained in citronella oil are active substances 
that have potential as antimicrobials. The aims of this study were to formulate a 
citronella oil nanoemulsion, to determine the characteristics of a citronella oil 
nanoemulsion, and to determine the antimicrobial activity of a citronella oil 
nanoemulsion using the well method. The nanoemulsion formulation was made using a 
low energy method. The nanoemulsion formulation was made with 3 variations of oil 
concentration, namely F1 (2%), F2 (4%), and F3 (6%), in addition, an emulsion 
formulation and diluted citronella oil were also made with a concentration similar to 
that of the nanoemulsion. Surfactants and cosurfactants used to make nanoemulsions 
were tween 80 and propylene glycol in a ratio of 20:15. The characterization of 
nanoemulsion preparations included measurement of particle distribution, organoleptic 
test, measurement of percent transmittance, measurement of viscosity, measurement of 
pH, examination of nanoemulsion type, freeze-thaw cycle test, and centrifugation test. 
The particle size of all nanoemulsion formulations of citronella oil with a concentration 
of 2% of 17.21nm(100%) 4% of 22.89 nm(100%), and 6% of 77.60 nm(15.5%) and 
16.43 nm (84.5%). The results of the organoleptic test characterization of nanoemulsion 
preparations showed the greater the concentration of oil, the more yellow the color, the 
more fragrant the smell, and the reduced clarity. The percent transmittance values of 
citronella oil nanoemulsion at concentrations of 2, 4, and 6%, respectively, were 
99.230±0.058; 98.030±0.058; and 97.500±0.000% while the emulsion with a similar 
concentration was 99.100±0.000; 71,300±0.000; and 54.530±0.058%. The viscosity 
value of citronella oil nanoemulsion at concentrations of 2, 4, and 6%, respectively, was 
6.0924; 11.3418; and 16.1668 cP. The type of nanoemulsion produced is the O/W type. 
The pH values of the citronella oil nanoemulsion with concentrations of 2, 4, and 6%, 
respectively, were 5.913±0.011; 5.906±0.006; and 5.873±0.012. The results of the 
centrifugation and freeze-thaw cycle test of citronella oil nanoemulsion showed a good 
level of physical stability. In general, the characteristics and stability of the 
nanoemulsion were better than the emulsion. The results of the centrifugation test of 
citronella oil emulsion and nanoemulsion did not show precipitation or phase 
separation. The antibacterial inhibition zone of P. acne nanoemulsion was 2, 4, 6%, 
respectively, namely 1,530±0.742; 2,500±0.354; and 6.180±1,450 mm. The antifungal 
inhibition zones of C. albicans nanoemulsion were 2, 4, 6%, respectively, namely 
0.000±0.000; 2.750±0.354; and 6.050±0.071 mm

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