Artikel Ilmiah : P2BA10019 a.n. ARIF MULYANTO, S.Si
| NIM | P2BA10019 |
|---|---|
| Namamhs | ARIF MULYANTO, S.Si |
| Judul Artikel | STUDI PATOLOGI PENYAKIT INFEKSI BAKTERIAL IKAN GURAMI (Osphronemus gouramy, Lac.) |
| Abstrak (Bhs. Indonesia) | Arif Mulyanto, Program Studi Magister Biologi, Program Pascasarjana Universitas Jenderal Soedirman, Studi Patologi Penyakit Infeksi Bakterial Ikan Gurami (Osphronemus gouramy Lac.). Pembimbing: Prof. Agus Irianto, M.Sc. Ph.D dan Gratiana E. Wijayanti, M.Rep.Sc. Ph.D. Kematian ikan gurami secara massal terjadi pada bulan Agustus sampai Desember 2010. Keadaan ini sangat merugikan para petani ikan karena kematian terjadi pada berbagai tahap perkembangan mulai dari ukuran 4 ± 2 cm sampai 35 ± 2 cm. Pemeriksaan karakter morfologi dan gejala klinis menunjukkan bahwa kematian ikan gurami disebabkan oleh infeksi bakteri. Guna mengetahui penyebab pasti kematian ikan gurami tersebut, maka penelitian ini dilakukan dengan tujuan untuk: 1) mengkonfirmasi bahwa kematian ikan gurami disebabkan oleh infeksi bakteri, 2) mengidentifikasi spesies bakteri penyebab kematian ikan, 3) mengevaluasi patogenitas bakteri yang diisolasi, dan 4) mengevaluasi gambaran histopatologi ikan yang terinfeksi di daerah terjadinya kematian ikan secara massal. Sampel ikan yang terinfeksi diambil secara purposive sampling. Jaringan yang terluka diambil untuk identifikasi bakteri berdasarkan morfologi koloni dan karakteristik biokimiawi. Ikan dibedah, organ ginjal dan hepar diambil dan difiksasi dalam larutan netral buffered formalin untuk pemeriksaan histopatologi menggunakan metode paraffin dan pewarnaan Haematoxyline-eosin. Patogenitas bakteri dievaluasi melalui uji postulat Koch. Hasil penelitian menunjukkan bahwa kematian ikan gurami secara massal disebabkan oleh infeksi bakteri. Teridentifikasi empat spesies bakteri yaitu Aeromonas hydrophila, Methylobacterium mesophilicum, Photobacterium damselae, dan Pseudomonas luteola. Tiga isolat bakteri tersebut memiliki patogenitas tinggi. Pada ikan yang terinfeksi parah, organ ginjal dan hati mengalami perubahan morfologi dan tekstur. Pemeriksaan histologi menunjukkan bahwa epitel tubulus renalis ginjal mengalami nekrosis dan terpisah dari membran basal, jaringan haematopoetic dan glomerulus mengalami kerusakan. Pada organ hepar, banyak sel hepatosit yang mengalami nekrosis, di daerah tertentu banyak sel hepatosit mengalami kerusakan dan terdegradasi sehingga menyisakan area kosong. Haemosiderin dalam berbagai ukuran dijumpai pada organ ginjal dan hepar. Hasil penelitian ini mengkonfirmasi bahwa kematian ikan gurami secara massal disebabkan oleh infeksi bakteri. |
| Abtrak (Bhs. Inggris) | Arif Mulyanto, Master of Biology, Postgraduate Program, Jenderal Soedirman University. Histopathological Study in Bacterial Infected Giant Gouramy (Osphronemus gouramy Lac.). Supervisors: Prof. Agus Irianto, M.Sc. Ph.D and Gratiana E. Wijayanti, M.Rep.Sc. Ph.D An outbreak of giant gouramy death was occured in a period of August to December 2010. This contribute a significant lost to the fish farmer since the death occured in a wide range of developmental stages from size of 4 ± 2 cm to 35 ± 2 cm. Examination on morphological characteristic and clinical symptom sugested that the death of the giant gouramy coused by bacterial infection. To reveal the couse of death of the giant gouramy this research was conducted with emphasis on these objectives: 1) to confirm that the death of the fish is coused by bacterial infection, 2) to identify the bacteria cousing the death, 3) to evaluate the pathogenesity of the isolated bacteria, and 4) to examine histophatological features of the infected fish from the outbreak area. The infected fish were collected according to purposive sampling. Wounded tissues were collected for bacterial identification based on collony morphology and biochemical characteristic. The fish were disected, their kidney and liver were removed and fixed in neutral buffered formalin for hiatopathological examination using paraffin embedded section and haematoxyline-eosin staining. Bacterial pathogenesity was evaluated by Koch postulate. There results confirmed that the death of the giant gouramy during the ourbreak was coused by bacterial infection. Four species of bacteria namely Aeromonas hydrophila, Methylobacterium mesophilicum, Photobacterium damselae, and Pseudomonas luteola were identified from the infected fish. Three isolates of these bacteria were highly pathogennic. In severely infected fish, the kidney and liver were altered in their morfological appearance and texture. Histological examination showed that the epithelia of the renal tubules were necrotic and were detached from the basal membran. The haematopoietic tissue and the glomerula were damaged. In the liver, many hepatocytes were necrotic, in particular area the damaged hepatocytes were degraded living an empty area. Haemosiderin in various size were observed in both kidney and liver. These fingding confirm that the outbreak of the giant gouramy death in the study area wes coused by bacterial infection. |
| Kata kunci | Aeromonas hydrophila, Methylobacterium mesophilicum, Photobacterium damselae, Pseudomonas luteola |
| Pembimbing 1 | Prof. Drs. Agus Irianto, MSc. Ph.D |
| Pembimbing 2 | Dra. Gratiana E. Wijayanti, M.Rep.Sc. Ph.D |
| Pembimbing 3 | |
| Tahun | 2014 |
| Jumlah Halaman | 14 |
| Tgl. Entri | 2014-08-06 15:08:20.574351 |