Artikel Ilmiah : H1A010013 a.n. DETRI LUSIANA
| NIM | H1A010013 |
|---|---|
| Namamhs | DETRI LUSIANA |
| Judul Artikel | AMOBILISASI EKSTRAK KASAR PAPAIN DARI TANGKAI PEPAYA JANTAN (Carica papaya L.) DENGAN POLIAKRILAMIDA DAN KARAKTERISASINYA |
| Abstrak (Bhs. Indonesia) | ABSTRAK Papain merupakan salah satu enzim proteolitik yang berasal dari tanaman pepaya. Papain umumnya masih digunakan dalam bentuk bebas sehingga hanya dapat digunakan untuk satu kali reaksi. Amobilisasi dilakukan agar enzim dapat dipakai berulang kali. Tujuan dari penelitian ini untuk mengisolasi, mengkarakterisasi dan menguji stabilitas papain amobil yang berasal dari tangkai pepaya jantan (Carica papaya L.). Penelitian diawali dengan isolasi ekstrak kasar papain. Ekstrak kasar enzim tersebut selanjutnya diamobilisasi dan dikarakterisasi meliputi karakterisasi suhu dan pH optimum enzim serta uji kestabilan penyimpanan dan pemakaian berulang. Pengujian tersebut berdasarkan uji aktivitas metode Kunitz yang dimodifikasi. Hasil penelitian menunjukan bahwa ekstrak kasar papain yang diamobilisasi dengan bahan pendukung poliakrilamida memiliki suhu dan pH optimum berturut-turut 50 °C dan pH 9 dengan aktivitasnya sebesar 0,497 dan 0,863 U/mL. Suhu penyimpanan papain amobil yang baik pada suhu 4 °C dengan waktu simpan maksimum selama 60 jam sisa aktivitas sebesar 52,9%. Pemakaian berulang papain amobil sampai empat kali dengan sisa aktivitas sebesar 58,9%. Kata kunci : amobilisasi enzim, papain, poliakrilamida |
| Abtrak (Bhs. Inggris) | ABSTRACT Papain is one of proteolytic enzymes originated form papaya. Papain is generally still use in the free form, so that it can only be used for one-time reaction. Immobilization was carried out, so that the enzyme can be use repeatedly. The purpose of this research was to isolate, characterize, test the stability of immobilized papain originated from male papaya stalk (Carica papaya L.). the research was started from the isolation of papain crude extract. The crude extract of enzyme was subsequently immobilized and characterized, in included temperature characterization and the optimum enzyme pH well as the storage stability and repeated use test. The test was based on the activity test of Kunitz method that was modified. The result of this research indicated the papain crude extract which was immobilized by supported material like polyacrylamide had optimum temperature and pH, it was 50oC and pH 9 consecutively with activity of 0,487 and 0,863 U/mL. The storage temperature of good immobilized papain was on 4oC with the maximum retention time for 60 hours and the residual activity was at 52,9%. The repeated use of immobilized papain was up to 4 times and the residual acitvity was at 58,9%. Keywords : immobilized enzyme, papain, polyacrylamide |
| Kata kunci | Kata kunci : amobilisasi enzim, papain, poliakrilamida |
| Pembimbing 1 | Zusfahair, S.Si, M.Si |
| Pembimbing 2 | Dian Riana Ningsih, S.Si, M.Si |
| Pembimbing 3 | |
| Tahun | 2014 |
| Jumlah Halaman | 57 |
| Tgl. Entri | 2014-08-02 20:31:59.161093 |