Artikel Ilmiah : G1F009032 a.n. SHIFAQ KHAIRUNNISA
| NIM | G1F009032 |
|---|---|
| Namamhs | SHIFAQ KHAIRUNNISA |
| Judul Artikel | ISOLASI DAN UJI SENSITIVITAS Bacillus spp. TERHADAP ANTIBIOTIK PADA TANAH PETERNAKAN DI BOYOLALI |
| Abstrak (Bhs. Indonesia) | Identifikasi dan uji sensitivitas bakteri Bacillus spp. hasil isolasi dari tanah daerah kontaminasi perlu dilakukan secara periodik. Bakteri Bacillus spp. dapat bertahan hidup di tanah selama bertahun-tahun dan tahan terhadap keadaan ekstrim. Tujuan penelitian ini untuk memperoleh Bacillus spp. yang mencemari sampel tanah peternakan di Kabupaten Boyolali dan mengetahui sensitivitas Bacillus spp. terhadap antibiotik. Penelitian ini dilakukan dengan metode observasional. Isolasi bakteri Bacillus spp. menggunakan medium Nutrient Agar. Isolat sudah murni dipindahkan pada medium Nutrient Agar miring. Sebanyak 4 isolat Bacillus spp. yang dapat diisolasi. Uji sensitivitas antibiotik menggunakan metode difusi Kirby Bauer. Paper disk yang mengandung antibiotik (amoksisilin 25µg, doksisiklin 30µg, dan ciprofloksasin 5µg) diletakkan di atas permukaan media yang telah berisi isolat bakteri Bacillus spp. kemudian diinkubasi pada suhu 37°C selama 24 jam. Diameter zona hambat yang ada diukur dan dibandingkan dengan CLSI. Analisis data ditampilkan secara deskriptif. Hasil penelitian menunjukkan bahwa isolat Bacillus spp. resisten terhadap amoksisilin dan sensitif terhadap doksisiklin dan ciprofloksasin. |
| Abtrak (Bhs. Inggris) | Identification and sensitivity testing of Bacillus spp. isolated from contaminated soil should be performed periodically. Bacteria such as Bacillus spp. can survive in the soil for many years even against extreme conditions. The purpose of this research was to obtain Bacillus spp. in farming soil sample in the district of Boyolali and discover its sensitivity against antibiotics. The research method used was the observational method. The isolation of Bacillus spp. used Nutrient Agar medium. Pure isolates were transferred to slant Nutrient Agar medium. 4 isolates that could be isolated. The method used for the sensitivity testing was the Kirby-Bauer diffusion method. Paper discs containing antibiotics (amoxicilin 25µg, doxycycline 30µg, dan ciprofloxacine µg) was placed on the surface of bacteria containing media, then incubated for 24 hours at 37°C. Inhibition zone was measured and compared with CLSI. The data was analyzed with the descriptive method. Results of the study indicated that all of the isolates were resistant to amoxicillin, and all the isolates were sensitive against doxycycline and ciprofloxacine. |
| Kata kunci | Bacillus spp, antibiotik, uji sensitivitas, tanah |
| Pembimbing 1 | Iskandar Sobri, M.Biotech., Apt |
| Pembimbing 2 | Drs. Sunarto, MS.,MP |
| Pembimbing 3 | |
| Tahun | 2013 |
| Jumlah Halaman | 9 |
| Tgl. Entri | (belum diset) |