| NIM | A1D022037 |
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| Namamhs | PUTRI DEWI LARASATI |
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| Judul Artikel | PENGARUH APLIKASI BIO P60 TERHADAP KESEHATAN BENIH DAN PERTUMBUHAN AWAL BIBIT PADI CIHERANG |
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| Abstrak (Bhs. Indonesia) | Pemanfaatan metabolit sekunder Pseudomonas fluorescens P60 sebagai alternatif pengendalian patogen tular-benih yang efektif dan ramah lingkungan dilakukan untuk menghasilkan benih yang lebih sehat. Penelitian ini bertujuan untuk: (1) mengidentifikasi patogen tular-benih, (2) mengkaji pengaruh aplikasi Bio P60 terhadap kesehatan benih padi, serta (3) mengkaji pengaruh aplikasi Bio P60 dalam mendukung perkecambahan dan pertumbuhan bibit. Penelitian dilaksanakan di Laboratorium Perlindungan Tanaman, Laboratorium Agronomi dan Hortikultura, dan screen house, Fakultas Pertanian Universitas Jenderal Soedirman, Purwokerto, Januari – Juni 2026, Penelitian menggunakan rancangan acak lengkap faktorial pada tahap in vitro dan rancangan acak kelompok faktorial pada tahap in planta, dengan dua faktor perlakuan, yaitu jenis bahan dan konsentrasi (kontrol aquades, fungsida berbahan aktif Propineb 2 g/L, dan Bio P60 konsentrasi 15 dan 20 mL/L) serta lama perendaman benih (30 dan 60 menit) yang diulang dengan 4 ulangan. Variabel pengamatan terdiri atas patogen tular-benih, persentase benih terinfeksi, daya kecambah, kecepatan tumbuh, indeks vigor, potensi tumbuh maksimum, panjang akar, panjang tanaman, jumlah daun, bobot segar dan bobot kering. Hasil penelitian menunjukkan Drechslera oryzae, Alternaria padwickii, Fusarium sp., dan Rhizopus oryzae ditemukan pada benih padi Ciherang. Perlakuan Bio P60 konsentrasi 20 mL/L mampu menekan infeksi D. oryzae dan A. padwickii, masing-masing-masing sebesar 69,56 dan 42,85 % dibandingkan kontrol. Selain itu, perlakuan Bio P60 konsentrasi 20 mL/L juga dapat meningkatkan daya kecambah (15,24 %), potensi tumbuh maksimum (11,35 %), panjang akar (16,15 %), panjang tanaman (17,28 %) dan bobot kering (31,63 %) dibandingkan kontrol. Kombinasi Bio P60 konsentrasi 20 mL/L pada perendaman 30 menit mampu menghasilkan kecepatan tumbuh dan panjang akar terbaik dengan peningkatan sebesar 5,84 dan 21,61 % dibandingkan kontrol perendaman 30 menit. |
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| Abtrak (Bhs. Inggris) | The use of secondary metabolites of Pseudomonas fluorescens P60 as an alternative to effective and environmentally friendly seed-borne pathogen control is carried out to produce healthier seeds. This study aims to: (1) identify seed-borne pathogens, (2) examine the effect of Bio P60 application on rice seed health, and (3) examine the effect of Bio P60 application in supporting germination and seed growth. The research was carried out at the Plant Protection Laboratory, the Agronomy and Horticulture Laboratory, and the screen house, Faculty of Agriculture, Jenderal Soedirman University, Purwokerto, January – June 2026, The research used a complete factorial random design at the in vitro stage and a factorial group random design at the in plant stage, with two treatment factors, namely the type of material and the concentration (aquades control, function of the active ingredient Propineb 2 g/L, and Bio P60 concentration of 15 and 20 mL/L) and the duration of seed soaking (30 and 60 minutes) which was repeated with 4 replications. The observation variables consisted of seed-borne pathogens, percentage of infected seeds, germination, growth speed, vigor index, maximum growth potential, root length, plant length, number of leaves, fresh weight and dry weight. The results showed that Drechslera oryzae, Alternaria padwickii, Fusarium sp., and Rhizopus oryzae were found in Ciherang rice seeds. Bio P60 treatment with a concentration of 20 mL/L was able to suppress D. oryzae and A. padwickii infections, respectively by 69.56 and 42.85 % compared to controls. In addition, Bio P60 treatment with a concentration of 20 mL/L could also increase germination power (15.24 %), maximum growth potential (11.35 %), root length (16.15 %), plant length (17.28 %) and dry weight (31.63 %) compared to controls. The combination of Bio P60 concentration of 20 mL/L at 30-minute immersion was able to produce the best growth rate and root length with an increase of 5.84 and 21.61 % compared to the 30-minute immersion control. |
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| Kata kunci | Bio P60, Metabolit Sekunder, Pseudomonas fluorescens P60, Patogen Tular-Benih. |
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| Pembimbing 1 | Prof. Ir. Loekas Soesanto, M.S., Ph.D. |
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| Pembimbing 2 | Dini Sundari, S.P., M.Sc. |
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| Pembimbing 3 | |
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| Tahun | 2026 |
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| Jumlah Halaman | 18 |
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| Tgl. Entri | 2026-10-20 07:34:33.761421 |
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