| NIM | A1D022252 |
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| Namamhs | HANIFAH ESTRI AULIA |
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| Judul Artikel | KERAGAMAN GENETIK MUTAN BLACK GINGER (Kaempferia parviflora Wall. ex Baker) HASIL IRADIASI SINAR GAMMA BERDASARKAN PENANDA MOLEKULER SIMPLE SEQUENCE REPEATS (SSR) |
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| Abstrak (Bhs. Indonesia) | Peningkatan keragaman genetik pada tanaman Kencur hitam (Kaempferia parviflora) dilakukan melalui iradiasi sinar gamma yang dapat merubah susunan DNA yang keberhasilannya perlu dievaluasi menggunakan penanda molekuler yang tepat, salah satunya Simple Sequence Repeats (SSR). Penelitian ini bertujuan untuk mendapatkan informasi keragaman genetik tanaman mutan kencur hitam hasil iradiasi sinar gamma menggunakan data penanda molekuler SSR dan mengidentifikasi penanda molekuler SSR yang paling efektif dalam analisis keragaman genetik tanaman mutan kencur hitam hasil iradiasi sinar gamma. Penelitian dilaksanakan di Badan Tenaga Nuklir Nasional (BATAN), Greenhouse Exfarm Fakultas Pertanian, Laboratorium Pemuliaan Tanaman dan Bioteknologi, Laboratorium Kultur Jaringan Tanaman, Laboratorium Riset, dan Laboratorium Bioteknologi Peternakan IAB, Universitas Jenderal Soedirman, Purwokerto, yang berlangsung selama tujuh bulan sejak September 2025 hingga Maret 2026. Penelitian dilakukan menggunakan tanaman kontrol dan mutan hasil iradiasi pada beberapa dosis sinar gamma yang dianalisis menggunakan empat marka SSR (Kp03, Kp04, Kp07, dan Kp10). Data pola pita hasil elektroforesis dianalisis menggunakan metode Sequential Agglomerative Hierarchical and Nested (SAHN) dengan algoritma Unweighted Pair Group Method with Arithmetic Mean (UPGMA) pada perangkat lunak RStudio. Hasil penelitian menunjukkan bahwa seluruh marka bersifat monomorfik pada kedelapan sampel yang diuji, masing-masing menghasilkan Na = Ne = 1 dengan PIC = 0. Hasil ini menunjukkan bahwa berdasarkan keempat marka SSR yang digunakan, tidak terdeteksi keragaman genetik pada populasi mutan kencur hitam hasil iradiasi sinar gamma, dan belum ditemukan marka SSR yang efektif (PIC > 0) untuk digunakan sebagai dasar seleksi dalam program pemuliaan kencur hitam berbasis mutasi induksi. |
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| Abtrak (Bhs. Inggris) | The genetic diversity of Black Ginger (Kaempferia parviflora) can be enhanced through gamma-ray irradiation, which alters DNA composition; the success of this process needs to be evaluated using appropriate molecular markers, one of which is Simple Sequence Repeats (SSR). This study aimed to obtain information on the genetic diversity of gamma-irradiated Black Ginger mutants using SSR molecular marker data and to identify the most effective SSR molecular markers for genetic diversity analysis of these mutants. The research was conducted at the National Nuclear Energy Agency (BATAN), the Exfarm Greenhouse of the Faculty of Agriculture, the Plant Breeding and Biotechnology Laboratory, the Plant Tissue Culture Laboratory, the Research Laboratory, and the IAB Animal Biotechnology Laboratory, Jenderal Soedirman University, Purwokerto, over a seven-month period from September 2025 to March 2026. The study used control and gamma-irradiated mutant plants at several irradiation doses, which were analyzed using four SSR markers (Kp03, Kp04, Kp07, and Kp10). Band pattern data from electrophoresis were analyzed using the Sequential Agglomerative Hierarchical and Nested (SAHN) method with the Unweighted Pair Group Method with Arithmetic Mean (UPGMA) algorithm in RStudio software. The results showed that all markers were monomorphic across the eight samples tested, each yielding Na = Ne = 1 with PIC = 0. These findings indicate that, based on the four SSR markers used, no genetic diversity was detected in the gamma-irradiated Black Ginger mutant population, and no effective SSR marker (PIC > 0) has yet been identified for use as a selection basis in mutation-induced breeding programs for Black Ginger. |
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| Kata kunci | Kaempferia parviflora, SSR, molekuler |
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| Pembimbing 1 | Eka Oktaviani, S.Si., M.Biotech |
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| Pembimbing 2 | Wilis Cahyani, S.P., M.P. |
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| Pembimbing 3 | |
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| Tahun | 2026 |
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| Jumlah Halaman | 9 |
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| Tgl. Entri | 2026-08-24 11:34:12.055474 |
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