| NIM | A1D022238 |
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| Namamhs | IBA SANUBARI |
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| Judul Artikel | APLIKASI KONSORSIUM BAKTERI ENDOFIT SEBAGAI AGENS PENGINDUKSI KETAHANAN TANAMAN PISANG CAVENDISH TERHADAP INFEKSI BANANA BUNCHY TOP VIRUS (BBTV) |
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| Abstrak (Bhs. Indonesia) | Produktivitas pisang Cavendish (Musa acuminata AAA Group) di Indonesia masih sangat dibatasi oleh infeksi Banana bunchy top virus (BBTV) yang ditularkan oleh vektor Pentalonia nigronervosa. Pemanfaatan bakteri endofit dalam bentuk konsorsium merupakan alternatif pengendalian hayati yang ramah lingkungan untuk menginduksi ketahanan sistemik tanaman. Penelitian ini bertujuan untuk menganalisis efektivitas konsorsium bakteri endofit dalam meningkatkan ketahanan tanaman pisang Cavendish terhadap infeksi BBTV serta menentukan formulasi konsorsium terbaik. Penelitian dilaksanakan pada Agustus 2025–Juni 2026 di Laboratorium dan screenhouse Fakultas Pertanian, Universitas Jenderal Soedirman. Metode penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan empat perlakuan dan empat ulangan, yaitu: kontrol sehat (N0), kontrol sakit (N1), Konsorsium 1 (Serratia sp., Enterobacter sp., dan Stenotrophomonas sp. [N2]), serta Konsorsium 2 (Bacillus velezensis, Stenotrophomonas sp., dan Enterobacter sp. [N3]). Hasil penelitian menunjukkan bahwa Konsorsium 1 (N2) merupakan formulasi terbaik dalam menginduksi ketahanan tanaman. Perlakuan N2 secara nyata mampu memperlambat masa inkubasi hingga 30,75 HSI, menekan intensitas penyakit menjadi 75,00%, menurunkan nilai AUDPC menjadi 2,13 ± 0,25, serta mempertahankan pertumbuhan tinggi tanaman optimal sebesar 17,80 cm. Secara biokimia dan fisiologis, perlakuan N2 juga meningkatkan klorofil total (2.632,74 mg/L), akumulasi glukosa (65,38 g mol⁻¹), karbohidrat total (98,76 mg g⁻¹), kadar IAA internal (6,57 µg g⁻¹ FW), dan aktivitas enzim peroksidase adaptif sebesar 6,11 ΔA" " 〖"menit" 〗^(-1). Melalui analisis PCR, keberadaan pita DNA target BBTV terbukti negatif/tidak terdeteksi pada tanaman yang diberi perlakuan konsorsium bakteri endofit. |
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| Abtrak (Bhs. Inggris) | Cavendish banana (Musa acuminata AAA Group) productivity in Indonesia is highly constrained by Banana bunchy top virus (BBTV) infection, transmitted by the aphid vector Pentalonia nigronervosa. The application of endophytic bacterial consortia serves as an environmentally friendly biocontrol alternative to trigger induced systemic resistance. This study aimed to evaluate the effectiveness of endophytic bacterial consortia in enhancing Cavendish banana resistance to BBTV and to select the best consortium formulation. The research was conducted from August 2025 to June 2026 at the Laboratories and screenhouse of the Faculty of Agriculture, Jenderal Soedirman University. The experiment employed a Completely Randomized Design (CRD) with four treatments and four replications: healthy control (N0), infected control (N1), Consortium 1 (Serratia sp., Enterobacter sp., and Stenotrophomonas sp. [N2]), and Consortium 2 (Bacillus velezensis, Stenotrophomonas sp., and Enterobacter sp. [N3]). The results revealed that Consortium 1 (N2) was the most effective formulation for inducing plant resistance. The N2 treatment significantly delayed the disease incubation period to 30.75 days after inoculation, suppressed disease intensity to 75.00%, reduced the AUDPC value to 2.13 ± 0.25, and maintained optimal plant height at 17.80 cm. Biochemically and physiologically, N2 increased total chlorophyll (2,632.74 mg/L), glucose accumulation (65.38 g mol⁻¹), total carbohydrate (98.76 mg g⁻¹), endogenous IAA (6.57 µg g⁻¹ fresh weight), and adaptive peroxidase (POX) activity to 6.11 ΔA" " 〖"min" 〗^(-1). PCR analysis confirmed that the BBTV DNA target band was negative or undetected in the consortia-treated plants. |
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| Kata kunci | Banana bunchy top virus (BBTV), bakteri endofit, ketahanan tanaman, konsorsium bakteri, pisang Cavendish. |
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| Pembimbing 1 | Dr. Ruth Feti Rahayuniati, S.P., M.P. |
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| Pembimbing 2 | Muhammad Bachtiar M., S.P.,M.P |
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| Pembimbing 3 | |
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| Tahun | 2026 |
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| Jumlah Halaman | 12 |
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| Tgl. Entri | 2026-08-20 16:47:04.748963 |
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