Artikel Ilmiah : K1A022089 a.n. LAILA VIKA SARI
| NIM | K1A022089 |
|---|---|
| Namamhs | LAILA VIKA SARI |
| Judul Artikel | Formulasi dan Karakterisasi Nanoemulsi Fraksi Minyak Atsiri Daun Pala (Myristica fragrans) serta Uji Aktivitas Antioksidan Menggunakan Metode DPPH |
| Abstrak (Bhs. Indonesia) | Penyakit degeneratif adalah penyakit yang disebabkan oleh penurunan fungsi organ tubuh karena paparan radikal bebas berlebih yang melebihi kapasitas antioksidan endogen sehingga diperlukan antioksidan alami. Minyak atsiri daun pala (Myristica fragrans) diketahui mengandung senyawa aktif sebagai antioksidan alami, namun bersifat hidrofobik sehingga diperlukan teknologi nanoemulsi untuk meningkatkan kelarutan senyawa aktif. Penelitian ini bertujuan untuk mengetahui fraksi hasil destilasi fraksinasi pengurangan tekanan yang memiliki aktivitas antioksidan teraktif dan menentukan formula nanoemulsi terbaik. Metode penelitian meliputi destilasi fraksinasi pengurangan tekanan minyak atsiri daun pala, analisis senyawa menggunakan Gas Chromatography-Mass Spectrometry (GC-MS), uji aktivitas antioksidan menggunakan metode DPPH, formulasi menggunakan surfaktan Tween 80 dan ko-surfaktan propilen glikol, karakterisasi nanoemulsi serta uji aktivitas antioksidan nanoemulsi. Hasil destilasi fraksinasi diperoleh tiga fraksi dengan hasil GC-MS berturut-turut yaitu fraksi 1 (84,33%) didominasi golongan monoterpen, fraksi 2 (6,17%) didominasi golongan monoterpen, dan fraksi 3 (3,42%) didominasi golongan fenilpropanoid yaitu eugenol dan miristisin. Uji aktivitas antioksidan menunjukkan fraksi 3 memiliki aktivitas antioksidan teraktif dengan nilai IC50 sebesar 12,67 ppm, kemudian diformulasi menjadi nanoemulsi dengan variasi konsentrasi 0%, 1%, 3%, dan 5% menggunakan surfaktan Tween 80 (20%) dan ko-surfaktan propilen glikol (15%). Hasil karakterisasi menunjukkan FN 1 dan FN2 merupakan formula terbaik dengan nilai pH 6,9 dan 6,8, bertipe oil in water (o/w), viskositas 14,6 dan 38,8 mPa.s, persen transmitan 99,6 dan 99,3%, ukuran partikel dominan 14,28 nm dan 16,51 nm, serta stabil. Aktivitas antioksidan nanoemulsi lebih aktif daripada non-nanoemulsi. Nilai IC50 FR3 (1%), FR3 (3%), FN1, dan FN2 berturut-turut yaitu 13,19; 11,66; 12,42; dan 8,81 ppm yang termasuk dalam kategori sangat kuat |
| Abtrak (Bhs. Inggris) | Degenerative diseases are conditions caused by a decline in organ function due to excessive exposure to free radicals that exceeds the body’s endogenous antioxidant capacity, thereby necessitating the use of natural antioxidants. Essential oil from nutmeg leaves (Myristica fragrans) is known to contain active compounds that act as natural antioxidants; however, these compounds are hydrophobic, so nanoemulsion technology is required to enhance their solubility. This study aims to identify the fraction from pressure-reduced fractional distillation that exhibits the highest antioxidant activity and to determine the optimal nanoemulsion formulation. The research methods included pressure-reduced fractional distillation of nutmeg leaf essential oil, compound analysis using Gas Chromatography-Mass Spectrometry (GC-MS), antioxidant activity testing using the DPPH method, formulation using surfactant Tween 80 and co-surfactant propylene glycol, characterization nanoemulsion, and antioxidant activity testing of the nanoemulsions. The fractional distillation yielded three fractions with the following GC-MS results: fraction 1 (84.33%) was dominated by monoterpenes, fraction 2 (6.17%) was dominated by monoterpenes, and fraction 3 (3.42%) was dominated by phenylpropanoids, namely eugenol and myristicin. Antioxidant activity testing showed that fraction 3 had the highest antioxidant activity with an IC50 value of 12.67 ppm; it was then formulated into nanoemulsions with concentrations of 0%, 1%, 3%, and 5% using the surfactant Tween 80 (20%) and the co-surfactant propylene glycol (15%). Characterization results showed that FN1 and FN2 were the best formulations, with pH values of 6.9 and 6.8, an oil-in-water (o/w) type, viscosities of 14.6 and 38.8 mPa.s, transmittance percentages of 99.6% and 99.3%, dominant particle sizes of 14.28 nm and 16.51 nm, and were stable. The antioxidant activity of the nanoemulsions was higher than that of the non-nanoemulsions. The IC50 values for FR3 (1%), FR3 (3%), FN1, and FN2 were 13.19; 11.66; 12.42; and 8.81 ppm, respectively, which fall into the very strong category. |
| Kata kunci | antioksidan, destilasi fraksinasi vakum, DPPH, minyak atsiri daun pala, nanoemulsi. |
| Pembimbing 1 | Dr. Undri Rastuti, S.Si., M.Si. |
| Pembimbing 2 | Moch. Chasani, S.Si., M.Si. |
| Pembimbing 3 | |
| Tahun | 2026 |
| Jumlah Halaman | 118 |
| Tgl. Entri | 2026-08-12 10:31:50.563969 |