Artikel Ilmiah : L1C022011 a.n. SHUFI NURFAJRI SUGIONO
| NIM | L1C022011 |
|---|---|
| Namamhs | SHUFI NURFAJRI SUGIONO |
| Judul Artikel | Skrining Dan Identifikasi Mikroba Laut Asal Methane Seep Perairan Sabang Sebagai Pendegradasi Polycyclic Aromatic Hydrocarbons (PAH) Berdasarkan Gen 16S rRNA |
| Abstrak (Bhs. Indonesia) | Pencemaran laut oleh minyak bumi yang mengandung Polycyclic Aromatic Hydrocarbons (PAH) merupakan masalah lingkungan global yang bersifat persisten, toksik, dan karsinogenik, sementara informasi mengenai mikroba pendegradasi PAH asal methane seep di Perairan Sabang masih terbatas. Penelitian ini bertujuan untuk mengetahui potensi bakteri methane seep sebagai pendegradasi PAH serta mengidentifikasi spesiesnya berdasarkan gen 16S rRNA. Metode yang digunakan adalah eksperimental terhadap 75 isolat bakteri koleksi Laboratorium Mikrobiologi Laut BRIN yang diskrining menggunakan sublimation test terhadap senyawa dibenzothiophene, fluorene, dan phenanthrene, dilanjutkan dengan pewarnaan Gram, ekstraksi DNA, amplifikasi PCR gen 16S rRNA, serta analisis BLAST dan pohon filogenetik menggunakan MEGA12. Hasil penelitian menunjukkan bahwa dari 75 isolat tersebut, diperoleh 8 isolat pendegradasi dibenzothiophene, 6 isolat fluorene, dan 6 isolat phenanthrene yang membentuk zona bening, dengan 4 isolat terbaik (MBAC24-PAH-26, -27, -31, dan -32) yang mampu mendegradasi ketiga senyawa tersebut. Isolat MBAC24-PAH 26 teridentifikasi sebagai Bacillus firmus dengan kemiripan 99,65% dan nilai bootstrap 98. Penelitian ini menyimpulkan bahwa methane seep Perairan Sabang menyimpan bakteri Gram positif berpotensi tinggi sebagai agen bioremediasi PAH di lingkungan laut. |
| Abtrak (Bhs. Inggris) | Marine pollution caused by petroleum containing Polycyclic Aromatic Hydrocarbons (PAH) was a global environmental problem that remained persistent, toxic, and carcinogenic, while information on PAH-degrading microbes originating from methane seeps in Sabang Waters was still limited. This study aimed to determine the potential of methane seep bacteria as PAH degraders and to identify their species based on the 16S rRNA gene. The method used was experimental, applied to 75 bacterial isolates from the collection of the Marine Microbiology Laboratory, BRIN, which were screened using a sublimation test against dibenzothiophene, fluorene, and phenanthrene, followed by Gram staining, DNA extraction, PCR amplification of the 16S rRNA gene, and BLAST and phylogenetic tree analysis using MEGA12. The results showed that among the 75 isolates, 8 isolates degraded dibenzothiophene, 6 isolates degraded fluorene, and 6 isolates degraded phenanthrene, as indicated by the formation of clear zones, with 4 of the best isolates (MBAC24-PAH-26, -27, -31, and -32) being able to degrade all three compounds. Isolate MBAC24-PAH-26 was identified as Bacillus firmus with a 99.65% similarity and a bootstrap value of 98. This study concluded that the methane seep in Sabang Waters harbored Gram-positive bacteria with high potential as PAH bioremediation agents in the marine environment. |
| Kata kunci | Kata kunci: Bacillus firmus, Gen 16S rRNA, Methane Seep, Perairan Sabang, Polycyclic Aromatic Hydrocarbons (PAH) // Keywords: 16S rRNA gene, Bacillus firmus, Methane seep, Polycyclic Aromatic Hydrocarbons (PAH), Sabang Waters. |
| Pembimbing 1 | Dr. rer. nat. Riyanti, S.T. M. Biotech |
| Pembimbing 2 | Dr. Ariani Hatmanti S.Si., M.Si. |
| Pembimbing 3 | - |
| Tahun | 2026 |
| Jumlah Halaman | 83 |
| Tgl. Entri | 2026-08-10 13:19:09.638281 |