| NIM | I1C022107 |
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| Namamhs | LURINDA BETA ASYFIA |
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| Judul Artikel | Pengaruh Nanostructured Lipid Carriers (NLC) Ekstrak Etanol Sambiloto (Andrographis panicula) terhadap Ekspresi mRNA IL-1β pada Hati Mencit yang Diinduksi CCl4 |
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| Abstrak (Bhs. Indonesia) | Kerusakan hati merupakan kondisi serius yang sering dipicu oleh paparan senyawa hepatotoksik, salah satunya karbon tetraklorida (CCl4) yang memicu stres oksidatif dan respon inflamasi hati dengan ditandai adanya peningkatan ekspresi sitokin proinflamasi, terutama interleukin-1β (IL-1β). Sambiloto (Andrographis paniculata) diketahui memiliki aktivitas antiinflamasi. Namun, rendahnya kelarutan Andrografolida sebagai senyawa marker sambiloto, mendorong pengembangan sistem penghantaran berbasis nanoteknologi, salah satunya Nanostructured Lipid Carriers (NLC) yang berpotensi meningkatkan kelarutan zat aktifnya. Namun, pengaruh NLC esktrak etanol sabiloto (EES) terhadap modulasi respon inflamasi hati, khususnya ekspresi mRNA IL-1β belum pernah diteliti. Oleh karena itu, penelitian ini dilakukan untuk mengkaji pengaruh NLC-EES terhadap ekspresi mRNA IL-1β pada hati mencit yang diinduksi CCl4. Penelitian eksperimental in vivo dilakukan pada 30 ekor mencit jantan galur BALB/c yang dibagi menjadi lima kelompok (n=6) menggunakan random sampling. Kelompok terdiri atas kontrol sehat, kontrol sakit (CCl4), NLC-EES dosis 70 mg/kgBB, ekstrak etanol sambiloto (EES) dosis 70 mg/kgBB, dan empty NLC. Induksi CCl4 diberikan pada hari pertama secara intraperitoneal pada dosis 1 mL/kgBB. Perlakuan dilakukan pada hari ketiga dan kelima secara sonde oral. Ekspresi relatif mRNA IL-1β dianalisis menggunakan qRT-PCR dengan metode Livaq (2−ΔΔCt). Seluruh kelompok perlakuan menunjukkan penurunan ekspresi relatif mRNA IL-1β dibandingkan dengan kelompok CCl4. Uji post hoc duun menunjukkan tidak terdapat perbedaan bermakna antarkelompok (p>0,05). Pemberian Nanostructured Lipid Carriers (NLC) ekstrak etanol sambiloto (Andrographis paniculata) mampu menurunkan ekspresi mRNA IL-1β pada inflamasi hati akut akibat induksi CCl4. |
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| Abtrak (Bhs. Inggris) | Liver damage is a serious condition often triggered by exposure to hepatotoxic compounds, one of which is carbon tetrachloride (CCl4), which causes oxidative stress and an inflammatory response in the liver, characterized by increased expression of proinflammatory cytokines, particulary interleukin-1β (IL-1β). Sambiloto (Andrographis paniculata) is known to have anti-inflammatory activity. However, the low solubility of Andrographolide as a marker compound of sambiloto has encouraged the development of nanotechnology-based delivery systems, one of which is Nanostructured Lipid Carriers (NLC), which has the potential to increase the solubility of its active ingredients. However, the effect of NLC ethanol extract of sambiloto (EES) on the modulation of the liver infalmmatory response, particularly IL-1β mRNA expression, has not been studied. Therefore, this study was conducted to examine the effect of NLC-EES on IL-1β mRNA expression in the liver of CCl4-induced mice. An in vivo experimental study was conducted on 30 male BALB/c mice divided into five groups (n=6) using random sampling. The groups consisted of healthy controls, sick controls (CCl4), NLC-EES at a dose of 70 mg/kgBW, ethanol extract of sambiloto (EES) at a dose of 70 mg/kgBW, and empty NLC. CCl4 induction was administered on the first day intraperitoneally at a dose of 1 mL/kgBW. Treatment was adminstered on the third and fifth days via oral sonde. Relative expression of IL-1β mRNA was analyzed using the qRT-PCR with the Livaq method (2−ΔΔCt). All treatment groups showed a decrease in relative IL-1β mRNA expression compared to the CCl4 group. Post hoc dunn tests showed no significant differences between groups (p>0,05). Administration of Nanostructured Lipid Carriers (NLC) of ethanol extract of Andrographis paniculata was able to reduced IL-1β mRNA expression in acute liver inflammation induced by CCl4. |
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| Kata kunci | Carbon tetrachloride, IL-1β, mRNA, Nanostructured Lipid Carrier, Sambiloto. |
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| Pembimbing 1 | apt. Dhadhang Wahyu Kurniawan, M.Sc., Ph.D. |
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| Pembimbing 2 | Dr. dr. Dody Novrial, M.Si. Med., Sp.PA. |
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| Pembimbing 3 | |
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| Tahun | 2026 |
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| Jumlah Halaman | 10 |
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| Tgl. Entri | 2026-02-23 14:07:09.325385 |
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