| NIM | G1A022042 |
|---|
| Namamhs | TITI SYARIFATUL ISTIQOMAH |
|---|
| Judul Artikel | UJI AKTIVITAS ANTIBAKTERI DAN ANTIBIOFILM NANOPARTIKEL PERAK (AgNP) DARI EKSTRAK ETIL ASETAT TERONG UNGU (Solanum melongena L.) TERHADAP Staphylococcus epidermidis ATCC 35984 |
|---|
| Abstrak (Bhs. Indonesia) | Latar Belakang: Staphylococcus epidermidis merupakan bakteri oportunistik penyebab Healthcare Associated Infections (HAIs) yang memiliki kemampuan membentuk biofilm, sehingga meningkatkan resistensi terhadap terapi antibiotik konvensional. Peningkatan kejadian resistensi antibiotik mendorong pengembangan agen alternatif berbasis bahan alam, salah satunya terong ungu (Solanum melongena L.) yang mengandung senyawa bioaktif. Tujuan: Penelitian ini bertujuan untuk mengevaluasi aktivitas antibakteri dan antibiofilm nanopartikel perak yang disintesis dari ekstrak etil asetat terong ungu (Solanum melongena L.) terhadap Staphylococcus epidermidis ATCC 35984. Metodologi: Penelitian dilakukan secara true experimental laboratory research dengan rancangan post-test only control group design. Penelitian ini menggunakan ekstrak etil asetat terong ungu yang diperoleh melalui metode maserasi, kemudian disintesis menjadi nanopartikel perak menggunakan metode green synthesis dengan ekstrak terong ungu sebagai agen pereduksi dan penstabil. Hasil: Hasil penelitian menunjukkan bahwa nilai MIC dan MBC diperoleh pada konsentrasi 2 mg/ml yang mampu membunuh ≥ 99,9% sel planktonik. Uji antibiofilm menunjukkan nilai MBIC50 pada konsentrasi 2 mg/ml, meskipun tidak berbeda bermakna secara statistik. Nilai MBRC50 tidak dapat ditetapkan karena tidak terdapat konsentrasi yang mampu mereduksi biofilm sebesar 50%. Kesimpulan: Nanopartikel perak ekstrak etil asetat terong ungu menunjukkan aktivitas antibakteri dan potensi antibiofilm terhadap S. epidermidis ATCC 35984, sehingga berpotensi dikembangkan sebagai agen antibakteri alternatif berbasis bahan alam. |
|---|
| Abtrak (Bhs. Inggris) | Background: Staphylococcus epidermidis is an opportunistic bacteria that causes healthcare-associated infections (HAIs) which has the ability to form biofilms, thus increasing resistance to conventional antibiotic therapy. The increasing incidence of antibiotic resistance encourages the development of alternative agents based on natural ingredients, one of which is purple eggplant (Solanum melongena L.) which contains bioactive compounds. Objective: This study aimed to evaluate the antibacterial and antibiofilm activity of silver nanoparticles synthesized from ethyl acetate extract of purple eggplant (Solanum melongena L.) against Staphylococcus epidermidis ATCC 35984. Methodology: The study was conducted as a true experimental laboratory research with a post-test only control group design. This study used ethyl acetate extract of purple eggplant obtained through the maceration method, then synthesized into silver nanoparticles using the green synthesis method with purple eggplant extract as a reducing and stabilizing agent. Results: The results showed that the MIC and MBC values were obtained at a concentration of 2 mg/ml which were able to kill ≥ 99.9% of planktonic cells. The antibiofilm test showed an MBIC50 value at a concentration of 2 mg/ml, although the difference was not statistically significant. The MBRC50 value could not be determined because no concentration was able to reduce the biofilm by 50%. Conclusion: Silver nanoparticles from ethyl acetate extract of purple eggplant showed antibacterial activity and antibiofilm potential against S. epidermidis ATCC 35984, thus having the potential to be developed as an alternative antibacterial agent based on natural ingredients. |
|---|
| Kata kunci | Antibakteri, Antibiofilm, Green Synthesis, Maserasi, Nanopartikel Perak (AgNP), Resistensi, Staphylococcus epidermidis ATCC 35984, Terong Ungu (Solanum melongena L.). |
|---|
| Pembimbing 1 | Dr. dr. Setiawati, M. Sc |
|---|
| Pembimbing 2 | Anriani Puspita Karunia Ning Widhi, S.Si., M. Si |
|---|
| Pembimbing 3 | - |
|---|
| Tahun | 2026 |
|---|
| Jumlah Halaman | 12 |
|---|
| Tgl. Entri | 2026-02-18 08:59:02.732278 |
|---|