| NIM | L1C021005 |
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| Namamhs | SAFIQOH NURHAMID |
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| Judul Artikel | Potensi Degradasi Polietilen oleh Bakteri Asal Sedimen Laut Dalam dari Perairan Indonesia |
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| Abstrak (Bhs. Indonesia) | Polietilen (PE) merupakan plastik yang sulit terurai secara alami dan berkontribusi besar terhadap pencemaran lingkungan laut. Biodegradasi oleh mikroorganisme menjadi alternatif ramah lingkungan yang berkelanjutan. Bakteri sedimen laut dalam berpotensi memiliki kemampuan mendegradasi polimer sintetis, namun masih jarang dieksplorasi di perairan Indonesia. Penelitian ini bertujuan untuk mengetahui kemampuan isolat bakteri sedimen laut dalam untuk mendegradasi PE serta mengidentifikasi bakteri potensial berdasarkan gen 16S rRNA. Isolat bakteri tunggal dan konsorsium bakteri diuji kemampuannya dalam mendegradasi PE menggunakan media Mineral Salt Medium (MSM) pada suhu 28 °C dengan kecepatan 130 rpm selama 90 hari. Hasil penelitian menunjukkan seluruh isolat mampu mendegradasi PE dengan tingkat efektivitas yang berbeda. Isolat SS521 asal Laut Sawu menunjukkan persentase degradasi tertinggi sebesar 4,110 ± 0,694 % dan berbeda signifikan dibandingkan kontrol. Analisis gen 16S rRNA mengidentifikasi isolat SS521 memiliki kedekatan tertinggi dengan Fictibacillus nanhaiensis (99,16 %). Hasil ini menunjukkan bakteri sedimen laut dalam berpotensi sebagai agen biodegradasi PE. |
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| Abtrak (Bhs. Inggris) | Polyethylene (PE) is a plastic material that is resistant to natural degradation and contributes significantly to marine environmental pollution. Microbial biodegradation represents a sustainable and environmentally friendly alternative. Deep-sea sediment bacteria potentially possess the ability to degrade synthetic polymers; however, their potential in Indonesian waters remains poorly explored. This study aimed to evaluate the ability of deep-sea sediment bacterial isolates to degrade PE and to identify potential PE-degrading bacteria based on 16S rRNA gene analysis. Single bacterial isolates and a bacterial consortium were tested for their ability to degrade polyethylene (PE) using Mineral Salt Medium (MSM) at 28 °C with agitation at 130 rpm for 90 days. The results showed that all isolates were able to degrade PE with varying levels of effectiveness. Isolate SS521 from the Sawu Sea exhibited the highest degradation percentage of 4.110 ± 0.694 % and was significantly different from the control. Based on 16S rRNA gene analysis, isolate SS521 showed the highest similarity to Fictibacillus nanhaiensis (99.16 %). These findings indicate that deep-sea sediment bacteria have potential as agents for environmentally friendly PE biodegradation. |
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| Kata kunci | Bakteri, Biodegradasi, Laut Dalam, Polietilen |
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| Pembimbing 1 | Dr.rer.nat. Riyanti, S.T, M.Biotech |
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| Pembimbing 2 | Nur Fitriah Afianti, S.Si., M.Si |
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| Pembimbing 3 | |
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| Tahun | 2026 |
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| Jumlah Halaman | 7 |
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| Tgl. Entri | 2026-02-17 16:04:40.575774 |
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