Artikel Ilmiah : G1A022014 a.n. KAMILIA SAFITRI FADHILAH
| NIM | G1A022014 |
|---|---|
| Namamhs | KAMILIA SAFITRI FADHILAH |
| Judul Artikel | PENGARUH NANOSTRUCTURED LIPID CARRIERS EKSTRAK ETANOL SAMBILOTO (Andrographis paniculata) TERHADAP KADAR ALT, AST, DAN BILIRUBIN MENCIT BALB/C YANG DIINDUKSI CCl4 |
| Abstrak (Bhs. Indonesia) | Latar Belakang: Penyakit hati merupakan masalah kesehatan global yang dapat dipicu oleh paparan zat hepatotoksik, salah satunya karbon tetraklorida (CCl4), yang diketahui menimbulkan stres oksidatif dan meningkatkan kadar ALT, AST, bilirubin. Andrographis paniculata (sambiloto) mengandung andrographolide dan flavonoid dengan aktivitas antioksidan yang berpotensi menekan stres oksidatif pada jaringan hati, meskipun pemanfaatannya masih terbatas akibat rendahnya bioavailabilitas. Pengembangan sistem penghantaran berbasis Nanostructured Lipid Carriers (NLC) diharapkan mampu meningkatkan stabilitas dan efektivitas senyawa aktif sambiloto. Tujuan: Penelitian ini bertujuan untuk mengetahui pengaruh NLC ekstrak etanol sambiloto terhadap kadar ALT, AST, dan bilirubin mencit BALB/c yang diinduksi CCl4. Metodologi: Penelitian ini merupakan studi eksperimental dengan desain post-test only with control group. Sebanyak 30 mencit BALB/c dibagi ke dalam lima kelompok, yaitu kontrol sehat, kontrol sakit yang diinduksi CCl4, serta tiga kelompok perlakuan yang diinduksi CCl4 dan diberikan NLC ekstrak etanol sambiloto, ekstrak etanol sambiloto, dan NLC kosong. Perlakuan diberikan pada hari ke-3 dan ke-5. Kadar ALT, AST, dan bilirubin total dianalisis menggunakan uji One-Way ANOVA dan Kruskal-Wallis dengan software statistik GraphPad Prism versi 10. Hasil: Kelompok yang menerima NLC ekstrak etanol sambiloto memiliki median kadar ALT sebesar 94 IU/L, serta rerata kadar AST dan bilirubin total masing-masing sebesar 122,2 IU/L dan 0,2275 mg/dL, yang lebih rendah dibandingkan dengan kelompok kontrol sakit. Secara statistik tidak ditemukan perbedaan bermakna kadar ALT (p = 0,2465), AST (p = 0,4829), dan bilirubin total (p = 0,1335) antar kelompok. Kesimpulan: Pemberian NLC ekstrak etanol sambiloto belum menunjukkan pengaruh yang signifikan terhadap parameter fungsi hati pada mencit yang diinduksi CCl4. |
| Abtrak (Bhs. Inggris) | Background: Liver disease is a global health problem that can be triggered by exposure to hepatotoxic agents, one of which is carbon tetrachloride (CCl4) known to induce oxidative stress and increase ALT, AST, and bilirubin levels. Andrographis paniculata (sambiloto) contains andrographolide and flavonoids with antioxidant activity that have the potential to suppress oxidative stress in hepatic tissue; however, its utilization remains limited due to low bioavailability. The development of a drug delivery system based on Nanostructured Lipid Carriers (NLC) is expected to enhance the stability and effectiveness of sambiloto’s active compounds. Objective: This study aimed to determine the effect of NLC containing ethanolic extract ofsambiloto on ALT, AST, and bilirubin levels in CCl4-induced BALB/c mice. Methodology: This study was conducted as an experimental study using a post-test only with control group design. A total of 30 BALB/c mice were divided into five groups: a healthy control group, a disease control group induced with CCl4, and three treatment groups induced with CCl4 and administered NLC-loaded ethanol extract of sambiloto, ethanol extract of sambiloto, and empty NLC, respectively. Treatments were administered on days 3 and 5. ALT, AST, and total bilirubin levels were analyzed using Kruskal-Wallis tests and One-Way ANOVA with GraphPad Prism version 10. Results: The group receiving NLC of ethanol extract of Andrographis paniculata had a median ALT level of 94 IU/L, as well as mean AST and total bilirubin levels of 122,2 IU/L and 0,2275 mg/dL, respectively, which were lower than those of the disease control group. However, no statistically significant differences are observed in ALT (p = 0,2465), AST (p = 0,4829), or total bilirubin levels (p = 0,1335) among the groups. Conclusion: Administration of NLC-loaded ethanol extract of sambiloto does not show a significant effect on liver function parameters in CCl4-induced mice. |
| Kata kunci | ALT, AST, bilirubin, CCl4, Nanostructured Lipid Carrier (NLC), Sambiloto (Andrographis paniculata). |
| Pembimbing 1 | Dr. dr. Nur Signa Aini Gumilas, M.Biotech |
| Pembimbing 2 | Apt. Dhadhang Wahyu Kurniawan, S.Si., M.Sc., Ph.D. |
| Pembimbing 3 | |
| Tahun | 2026 |
| Jumlah Halaman | 99 |
| Tgl. Entri | 2026-01-09 19:01:56.562209 |