| NIM | G1A022095 |
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| Namamhs | NUR MUTIA TANJUNG |
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| Judul Artikel | PENGARUH PAPARAN SUB KRONIK PB ASETAT TERHADAP KADAR SUPEROXIDE DISMUTASE (SOD) PADA HEPAR TIKUS WISTAR JANTAN (Rattus norvegicus) |
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| Abstrak (Bhs. Indonesia) | Latar Belakang: Timbal (Pb) merupakan logam berat yang memiliki sifat toksisitas tinggi yang mampu meningkatkan ROS sehingga menurunkan aktivitas antioksidan enzimatik seperti superoksida dismutase (SOD). SOD akan mengkatalisis anion superoksida (O2-) dan hidrogen (H) menjadi hidrogen peroksida (H2O2). Tujuan: Mengetahui pengaruh dosis dan durasi paparan sub-kronik Pb (timbal) terhadap kadar antioksidan superoxide dismutase (SOD) pada tikus Wistar jantan (Rattus norvegicus). Metode: Penelitian ini menggunakan desain posttest only control group design dengan hewan coba tikus putih galur Wistar jantan (Rattus norvegicus) sebanyak 45 ekor tikus. Kelompok perlakuan dibagi menjadi 3 durasi yaitu 35, 40, dan 45 hari yang masing masing kelompok terbagi dalam 5 perlakuan yaitu aquadest, 10mg/kgBB, 25mg/kgBB, 50mg/kgBB, dan 75mg/kgBB. Induksi Pb asetat melalui rute peroral. Pemeriksaan kadar SOD menggunakan metode ELISA. Hasil: Hasil uji Oneway ANOVA menunjukkan bahwa durasi paparan timbal memberikan pengaruh yang signifikan dengan nilai p <0,05 pada perlakuan selama 35, 40 dan 45 hari. Pada dosis paparan timbal tidak menunjukkan pengaruh yang signifikan dengan p>0,05 pada kelompok kontrol dan 10mg/kgBB, dan signifikan p<0,05 pada kelompok 25mg/kgBB, 50mg/kgBB, dan 75mg/kgBB terhadap kadar superoksida dismutase (SOD). Kesimpulan: Paparan timbal berbagai dosis dan durasi terbukti menurunkan kadar SOD hepar tikus Wistar jantan (Rattus norvegicus). Paparan timbal secara signifikan menurunkan kadar SOD mulai dosis 25mg/kgBB selama 35, 40, dan 45 hari. |
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| Abtrak (Bhs. Inggris) | Background: Lead (Pb) is a heavy metal with high toxicity that can elevate ROS, thereby reducing the activity of enzymatic antioxidants such as superoxide dismutase (SOD). SOD catalyzes the conversion of superoxide anions (O₂⁻) and hydrogen (H) into hydrogen peroxide (H₂O₂). Objective: This study aimed to evaluate the effects of dose and duration of sub-chronic lead (Pb) exposure on hepatic superoxide dismutase (SOD) levels in male Wistar rats (Rattus norvegicus). Method :A posttest-only control group design was applied using 45 male Wistar rats. Animals were assigned to three exposure durations (35, 40, and 45 days), each consisting of five treatment groups: aquadest (control), 10 mg/kg BW, 25 mg/kg BW, 50 mg/kg BW, and 75 mg/kg BW. Lead acetate was administered orally. SOD levels were measured using the ELISA method. Results: The One-Way ANOVA results showed in lead exposure durations had a significant effect on superoxide dismutase (SOD) levels at treatment durations of 35, 40, and 45 days (p<0.05). Based on the dose groups, no significant effect was observed in the control group or in the 10 mg/kgBW dose group (p>0.05). However, significant effects were found in the 25 mg/kgBW, 50 mg/kgBW, and 75 mg/kgBW dose groups (p<0.05), indicating that higher lead exposure doses were associated with significant changes in SOD levels. Conclusion: Sub-chronic lead exposure at various doses and durations significantly reduced hepatic SOD levels in male Wistar rats. Significant decreases were observed starting at 25 mg/kg BW across all exposure durations (35, 40, and 45 days). |
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| Kata kunci | Kata kunci: reactive oxygen species (ROS), stress oksidatif, superoksida dismutase (SOD), timbal (Pb) |
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| Pembimbing 1 | 3. Dr. dr. Agung Saprasetya Dwi Laksana, M.Sc. PH |
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| Pembimbing 2 | dr. Yudhi Wibowo, M.P.H |
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| Pembimbing 3 | |
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| Tahun | 2025 |
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| Jumlah Halaman | 12 |
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| Tgl. Entri | 2026-01-06 15:23:23.502368 |
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