| NIM | G1A022069 |
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| Namamhs | NAZEYA ZAFIRA |
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| Judul Artikel | EFEK PEMBERIAN KEFIR SUSU KAMBING BERBAGAI DOSIS TERHADAP KADAR ALT DAN AST TIKUS PUTIH (Rattus norvegicus) GALUR WISTAR MODEL DIABETES MELITUS TIPE 2 |
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| Abstrak (Bhs. Indonesia) | Latar belakang: Diabetes melitus tipe 2 (DMT2) dapat disertai disfungsi hati yang tercermin dari perubahan enzim transaminase. Tujuan: Menganalisis efek pemberian kefir susu kambing berbagai dosis terhadap kadar alanine aminotransferase (ALT) dan aspartate aminotransferase (AST) pada tikus Wistar model DMT2. Metode: Penelitian eksperimental dengan lima kelompok: kontrol sehat (A), kontrol DMT2 (B), DMT2+kefir 1,05 mL/200 gBB (C), DMT2+kefir 2,1 mL/200 gBB (D), dan DMT2+kefir 4,2 mL/200 gBB (E). Induksi DMT2 dilakukan dengan pemberian diet tinggi lemak (otak sapi) 28 hari dan injeksi streptozotocin dua kali (30 mg/kgBB lalu 50 mg/kgBB). Pemeriksaan ALT dan AST dilakukan dengan metode kinetik enzimatik standar IFCC. Hasil: Rerata ALT (U/L) berturut-turut A–E adalah 73,67; 132,33; 257,25; 104,67; 115,75. Uji ANOVA (data ALT ditransformasi log10) menunjukkan perbedaan bermakna antar kelompok (p=0,004) dan uji lanjut Tukey menunjukkan perbedaan bermakna antara kelompok A dan C (p=0,002). Rerata AST (U/L) A–E adalah 119,17; 126,50; 228,00; 151,33; 137,25; uji ANOVA menunjukkan tidak bermakna (p=0,106). Kesimpulan: Pemberian kefir susu kambing dosis 1,05 mL/200 gBB berasosiasi dengan peningkatan ALT pada model DMT2, sedangkan perbandingan kadar AST antar kelompok tidak bermakna secara statistik. |
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| Abtrak (Bhs. Inggris) | Background: Type 2 diabetes mellitus (T2DM) may be accompanied by liver dysfunction reflected by altered transaminase enzymes. Objective: To analyze the effects of different doses of goat milk kefir on alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels in a T2DM Wistar rat model. Methods: A laboratory experimental study with five groups: healthy control (A), T2DM control (B), T2DM+kefir 1.05 mL/200 gBW (C), T2DM+kefir 2.1 mL/200 gBW (D), and T2DM+kefir 4.2 mL/200 gBW (E). T2DM was induced using a high-fat diet (cow brain) for 28 days followed by two streptozotocin injections (30 mg/kgBW then 50 mg/kgBW). ALT and AST were measured using an IFCC-based kinetic enzymatic method. Results: Mean ALT (U/L) for groups A–E were 73.67, 132.33, 257.25, 104.67, and 115.75, respectively. One-way ANOVA on log10-transformed ALT showed significant differences among groups (p=0.004), and Tukey post-hoc revealed a significant difference between groups A and C (p=0.002). Mean AST (U/L) for groups A–E were 119.17, 126.50, 228.00, 151.33, and 137.25; ANOVA showed no significant difference (p=0.106). Conclusion: Goat milk kefir at 1.05 mL/200 gBW was associated with increased ALT in the T2DM model, while AST did not differ significantly across groups.
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| Kata kunci | ALT, AST, DMT2, kefir susu kambing |
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| Pembimbing 1 | dr. Alfi Muntafiah, M.Sc. |
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| Pembimbing 2 | Novia Yunika, S.Si, M.Biomed. |
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| Pembimbing 3 | |
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| Tahun | 2025 |
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| Jumlah Halaman | 11 |
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| Tgl. Entri | 2025-12-30 13:14:45.280489 |
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