Artikel Ilmiah : B1B021001 a.n. YUNITA PUSPITASARI
| NIM | B1B021001 |
|---|---|
| Namamhs | YUNITA PUSPITASARI |
| Judul Artikel | Genetic Profiling of Several Nepenthes Species Based on RAPD Markers |
| Abstrak (Bhs. Indonesia) | Nepenthes is the only genus in the family Nepenthaceae, comprising about 120 species of tropical carnivorous plants. These plants grow in nutrient-poor habitats such as peat swamp forests, mangroves, and montane forests. Nepenthes obtain nitrogen by trapping and digesting insects using pitcher-shaped leaves with three zones: the peristome, which attracts prey; the slippery zone, which prevents escape; and the digestive zone, which absorbs nutrients. Indonesia is a major center of Nepenthes diversity, with many endemic species, making genetic diversity studies crucial for conservation. This research was conducted for four months at the Genetics and Molecular Laboratory, Faculty of Biology, Universitas Jenderal Soedirman. Six Nepenthes species were sampled, and their genomic DNA was extracted using the CTAB method. DNA quality and quantity were assessed with a nanodrop spectrophotometer and agarose gel electrophoresis. DNA amplification was performed using RAPD-PCR with eight primers (ES10G23, OPA-02, OPA-10, OPA-13, OPB-05, OPB-11, OPC-05, OPU-19). The resulting band patterns were scored as binary data (1 for presence, 0 for absence) and analyzed with MVSP software to construct a dendrogram of genetic relationships. The extraction produced high-quality DNA with concentrations of 250–630 ng/μL and A260/A280 ratios of 2.011–2.178. A total of 23 RAPD markers were obtained, 19 polymorphic, giving a polymorphism level of 81.7%, considered high. The UPGMA dendrogram showed that N. reinwardtiana is the most genetically distinct, while N. adrianii and N. mirabilis var. globosa are the closest. These results provide insights into genetic diversity and relationships among Nepenthes species, supporting conservation strategies. |
| Abtrak (Bhs. Inggris) | Nepenthes is the only genus in the family Nepenthaceae, comprising about 120 species of tropical carnivorous plants. These plants grow in nutrient-poor habitats such as peat swamp forests, mangroves, and montane forests. Nepenthes obtain nitrogen by trapping and digesting insects using pitcher-shaped leaves with three zones: the peristome, which attracts prey; the slippery zone, which prevents escape; and the digestive zone, which absorbs nutrients. Indonesia is a major center of Nepenthes diversity, with many endemic species, making genetic diversity studies crucial for conservation. This research was conducted for four months at the Genetics and Molecular Laboratory, Faculty of Biology, Universitas Jenderal Soedirman. Six Nepenthes species were sampled, and their genomic DNA was extracted using the CTAB method. DNA quality and quantity were assessed with a nanodrop spectrophotometer and agarose gel electrophoresis. DNA amplification was performed using RAPD-PCR with eight primers (ES10G23, OPA-02, OPA-10, OPA-13, OPB-05, OPB-11, OPC-05, OPU-19). The resulting band patterns were scored as binary data (1 for presence, 0 for absence) and analyzed with MVSP software to construct a dendrogram of genetic relationships. The extraction produced high-quality DNA with concentrations of 250–630 ng/μL and A260/A280 ratios of 2.011–2.178. A total of 23 RAPD markers were obtained, 19 polymorphic, giving a polymorphism level of 81.7%, considered high. The UPGMA dendrogram showed that N. reinwardtiana is the most genetically distinct, while N. adrianii and N. mirabilis var. globosa are the closest. These results provide insights into genetic diversity and relationships among Nepenthes species, supporting conservation strategies. |
| Kata kunci | Genetic Diversity, Genetic Relationships, Nepenthes, Polymorphism, RAPD |
| Pembimbing 1 | Ir. Alice Yuniaty, M.Sc., PhD. |
| Pembimbing 2 | Drs. Adi Amurwanto, M.Sc. |
| Pembimbing 3 | |
| Tahun | 2025 |
| Jumlah Halaman | 15 |
| Tgl. Entri | 2025-08-21 11:02:14.191338 |