| NIM | B1A021078 |
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| Namamhs | FARAH ISPRAMUDITA SEPTIYANTI |
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| Judul Artikel | Isolasi dan Identifikasi Molekuler Bakteri Amilolitik dari Kawasan Hulu Sungai Logawa Banyumas, Jawa Tengah Berdasarkan Gen 16S rRNA |
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| Abstrak (Bhs. Indonesia) | Penelitian ini bertujuan untuk melakukan isolasi dan skrining bakteri amilolitik dari air dan sedimen kawasan hulu Sungai Logawa dan melakukan identifikasi bakteri amilolitik berdasarkan gen 16S rRNA. Sebanyak 22 isolat bakteri telah berhasil diperoleh dari kawasan hulu Sungai Logawa. Skrining aktivitas amilolitik menunjukkan bahwa 8 dari 22 isolat bakteri berhasil mendegradasi 0,1% substrat amilum. Isolat SH 8 menunjukkan indeks amilolitik paling tinggi dengan nilai 1,08. Hasil pensejajaran gen 16S rRNA di NCBI menunjukkan bahwa isolat SH 8 memiliki similaritas tertinggi dengan Streptomyces lactacystinicus strain OM-6519T dengan persentase kemiripan sebesar 99,71%. Konstruksi pohon filogenetik menggunakan metode neighbor-joining dengan model Kimura 2-parameter menunjukkan bahwa isolat SH 8 memiliki hubungan kekerabatan paling dekat dengan Streptomyces lactacystinicus strain OM-6519T, keduanya membentuk klad monofiletik dengan nilai bootstrap sebesar 93 dan jarak genetik sebesar 0,001. Berdasarkan konfirmasi karakter morfologi, fisiologi, dan biokimia, isolat SH 8 termasuk ke dalam genus Streptomyces. Hasil penelitian menunjukkan bahwa sebanyak delapan bakteri amilolitik telah diperoleh dari air dan sedimen kawasan hulu Sungai Logawa dan berdasarkan identifikasi gen 16S rRNA, isolat SH 8 sebagai bakteri amilolitik potensial memiliki similaritas tertinggi dengan Streptomyces lactacystinicus strain OM-6519T. |
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| Abtrak (Bhs. Inggris) | This study aims to isolate and screening of amylolytic bacteria from the water and sediment from the upstream area of the Logawa River and identify amylolytic bacteria based on the 16S rRNA gene. A total of 22 bacterial isolates have been successfully obtained from the upstream area of the Logawa river. Screening of amylolytic showed that 8 out of 22 bacterial isolates successfully degraded 0.1% amylum substrate. Isolate SH 8 became a bacteria with the highest amylolytic index with a value of 1.08. The results of the 16S rRNA gene alignment at NCBI showed that isolate SH 8 had the highest similarities with Streptomyces lactacystinicus strain OM-6519T with a similarity percentage of 99.71%. Phylogenetic tree construction using the neighbor-joining method with the Kimura 2-parameter model shows that isolate SH 8 has the closely related to Streptomyces lactacystinicus strain OM-6519T, both forming a monophyletic with a bootstrap value of 93 and a genetic distance of 0,001. Based on the confirmation of morphological, physiological, and biochemical characters, isolate SH 8 belongs to the genus Streptomyces. The results showed that eight amylolytic bacteria were obtained by water and sediment from the upstream area of the Logawa river. Based on 16S rRNA gene identification, isolate SH 8, as a potential amylolytic bacteria, had the highest similarity to Streptomyces lactacystinicus strain OM-6519T. |
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| Kata kunci | amilase, bakteri amilolitik, gen 16S rRNA, identifikasi molekuler, Sungai Logawa |
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| Pembimbing 1 | Dr. Sri Martina Wiraswati, S.P., M.Si. |
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| Pembimbing 2 | Yuriza Eshananda, S.Si., M.Si. |
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| Pembimbing 3 | |
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| Tahun | 2025 |
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| Jumlah Halaman | 15 |
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| Tgl. Entri | 2025-08-19 17:38:59.758989 |
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