| NIM | A1D021116 |
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| Namamhs | ERIN SALSABILA FIRDAUSI |
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| Judul Artikel | ISOLASI, KARAKTERISASI DAN POTENSI Bacillus sp. ASAL RIZOSFER PUTRI MALU DATARAN MENENGAH UNTUK MENGENDALIKAN PENYAKIT ANTRAKNOSA PADA CABAI RAWIT |
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| Abstrak (Bhs. Indonesia) | Penelitian bertujuan untuk mengisolasi dan mengkarakterisasi bakteri Bacillus sp. asal rizosfer akar putri malu dataran menengah, menguji kemampuannya sebagai bakteri antagonis dan Plant Growth Promoting Bacteria (PGPR), serta kemampuannya mengendalikan Colletotrichum sp. pada cabai rawit secara in vivo. Bacillus sp. diisolasi dari tanah rizosfer putri malu daerah dataran menengah di Kecamatan Baturraden dan Kecamatan Mrebet. Karakterisasi bakteri hasil isolasi meliputi karakter morfologi koloni dan sel, pengujian gram, pengujian katalase, dan pengujian oksidase. Pengujian potensi antagonisme Bacillus sp. secara in vitro dilakukan dengan metode dual culture untuk jamur patogen Colletotrichum sp., Rhizoctonia sp., Fusarium sp., dan metode double layer untuk bakteri patogen Ralstonia sp. dan Xanthomonas sp. Pengujian secara in vivo dilakukan dengan metode detach fruit. Hasil penelitian didapatkan 12 isolat Bacillus sp. dengan karakteristik koloni yang beragam, yaitu berukuran 0,2-0,7 cm, berwarna putih-krim, berbentuk circular dan irregular, tidak tembus cahaya secara optik, elevasi flat sampai convex, tekstur umumnya kasar serta beberapa bertekstur kerut dan kering, dan memiliki tepi yang bervariasi antara serrate, filamen, entire, dan undulate. Hasil uji in vitro menunjukkan isolat BRM 1.3, BRM 2.1, dan BRM 2.2 mampu menghambat kelima patogen yang diuji dengan persentase daya hambat jamur patogen sebesar 42,73%-62,50% dan indeks penghambatan bakteri patogen sebesar 1,74-4,10. Pemberian Bacillus sp. belum mampu meningkatkan bobot kecambah dan panjang akar, tetapi beberapa isolat mampu meningkatkan bobot akar kecambah hingga 57,63%. Isolat Bacillus sp. yang diuji pada penelitian ini juga belum mampu mengendalikan penyakit antraknosa pada buah cabai rawit secara in vivo. |
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| Abtrak (Bhs. Inggris) | The study aimed to isolate and characterize Bacillus sp. from the rhizosphere of sensitive plant in midland areas, and to evaluate their potential as antagonistic bacteria, Plant Growth Promoting Bacteria (PGPR), and control Colletotrichum sp. in chili peppers under in vivo condition. Bacillus sp. was isolated from the rhizosphere of sensitive plants in Kecamatan Baturraden and Kecamatan Mrebet. Characterization included colony and cell morphology, Gram, catalase, and oxidase test. Antagonistic potential of Bacillus sp. was tested using the dual culture method for pathogenic fungi Colletotrichum sp., Rhizoctonia sp., and Fusarium sp., and the double layer method for pathogenic bacteria Ralstonia sp. and Xanthomonas sp. In vivo testing was conducted using the detach fruit method. Twelve Bacillus sp. isolates exhibited diverse colony characteristics, including sizes of 0.2-0.7 cm, white-cream coloration, circular and irregular shapes, optically opaque, flat to convex elevation, generally coarse texture and several with wrinkled and dry textures, and margins varying among serrate, filamentous, entire, and undulate. In vitro test showed that isolates BRM 1.3, BRM 2.1, and BRM 2.2 inhibited all five tested pathogens, with inhibition rates against fungal pathogens ranging from 42.73% to 62.50% and inhibition index against bacterial pathogens ranging from 1.74 to 4.10. The application of Bacillus sp. did not increase seedling weight and root length, but several isolates increased seedling root weight by up to 57.63%. The Bacillus sp. isolates tested in this study did not effectively control anthracnose disease in chili peppers under in vivo conditions. |
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| Kata kunci | Colletotrichum, cabai rawit, Bacillus, rizosfer, putri malu |
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| Pembimbing 1 | Dr. Endang Mugiastuti, S.P., M.P. |
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| Pembimbing 2 | Dr. Etik Wukir Tini, S.P., M.P. |
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| Pembimbing 3 | |
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| Tahun | 2025 |
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| Jumlah Halaman | 16 |
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| Tgl. Entri | 2025-07-24 16:27:09.558802 |
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