Artikel Ilmiah : G1B019018 a.n. RIYAN RISKI RAHARJA
| NIM | G1B019018 |
|---|---|
| Namamhs | RIYAN RISKI RAHARJA |
| Judul Artikel | Effect of Glass Ionomer Cement Combined with Bawor Durian rind Nanocellulose on Fibroblast Viability |
| Abstrak (Bhs. Indonesia) | Glass ionomer cement (GIC) merupakan bahan restorasi yang sering digunakan. GIC memiliki kelemahan seperti mudah larut dan rentan terhadap fraktur. Salah satu inovasi yang dilakukan untuk menutupi kekurangan tersebut dengan penambahan nanoselulosa kulit durian yang terbukti dapat meningkatkan sifat GIC. Namun efek toksisitas GIC yang dikombinasikan nanoselulosa kulit durian terhadap gingiva belum diteliti lebih lanjut. Toksisitas GIC kombinasi nanoselulosa kulit durian dapat diketahui dengan uji viabilitas fibroblas. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian glass ionomer cement yang dikombinasikan nanoselulosa kulit durian bawor (Durio zibethinus Murr.) konsentrasi 0,5%, 1%, dan 1,5% terhadap viabilitas fibroblas. Jenis penelitian ini berupa penelitian eksperimental laboratoris in vitro dengan rancangan post-test only control group design. Sampel berupa hasil kultur fibroblas BHK-21 dalam microplate well 96 yang dibagi menjadi 4 kelompok. Kelompok perlakuan pemberian elusi GIC nanoselulosa kulit durian konsentrasi 0,5%, 1%, dan 1,5% terhadap kultur fibroblas dan kontrol negatif berupa perlakuan pemberian elusi GIC tanpa nanoselulosa kulit durian. Uji karakterisasi nanoselulosa menggunakan Particle Size Analyser dan nilai viabilitas diperoleh dengan uji MTT Assay kemudian dibaca dengan microplate reader. Data dianalisis dengan uji One Way ANOVA diikuti dengan uji Post Hoc LSD. Hasil uji karakterisasi menunjukkan nanoselulosa kulit durian memiliki rata-rata 50,79 nm dengan rata-rata nilai zeta potensial sebesar -29,23 mV. Persentase viabilitas sel terendah bernilai 68, 69% pada kelompok paparan elusi GIC nanoselulosa kulit durian konsentrasi 1,5% terhadap fibroblas dan viabilitas tertinggi bernilai 86,82% pada kelompok paparan elusi GIC nanoselulosa kulit durian konsentrasi 0,5% terhadap fibroblas. Kesimpulan dari penelitian ini adalah terdapat pengaruh pemberian glass ionomer cement yang dikombinasikan nanoselulosa kulit durian bawor (Durio zibethinus Murr.) konsentrasi 0,5%, 1%, dan 1,5% terhadap viabilitas fibroblas. |
| Abtrak (Bhs. Inggris) | Background: Glass Ionomer Cement (GIC) is commonly used as restorative material. Weakness of GIC such as susceptibility to dissolution and fracture. The innovation to overcome these limitations is the addition of durian rind nanocellulose as GIC filler, wich has been shown to enhance GIC’s mechanical properties. However the toxicity effect of GIC combined with durian rind nanocellulose on the gingiva has not been studied further. The toxicity of GIC combined with durian rind nanocellulose can be determined by fibroblast viability testing. This study aims to determine the effect of glass ionomer cement combined with durian bawor rind nanocellulose (Durio zibethinus Murr.) at concentrations of 0,5%, 1%, dan 1,5% on fibroblast viability. Method: This study is in vitro laboratory experiment using a post-test only control group design. The sampels consisted of BHK-21 fibroblast culture in 96-well microplates, divide into four groups. Treatment group with 0,5%, 1%, and 1,5% durian rind nanocellulose GIC eluate expose to fibroblast culture, and negative control group exposed to GIC eluate without durian rind nanocellulose. Nanocellulose characterization was determined using Partile Size Analyzer and cell viability was measured using MTT Assay then read by microplate reader. Data were analyzed using One-Way ANOVA followed by the Post Hoc LSD test. Result: Characterization result showed that the average size of durian rind nanocellulose was 50,79 nm with an average of zeta potensial is -29,23 mV. The lowest cell viability percentage was 68.69% in 1,5% durian rind nanocellulose GIC eluate group while the highest was 86,82% in 0,5% durian rind nanocellulose GIC eluate group. Conclusion: The study concludes that there is an effect of glass ionomer cement combined with durian bawor rind nanocellulose (Durio zibethinus Murr.) at concentrations of 0,5%, 1%, dan 1,5% on fibroblast viability |
| Kata kunci | Durian rind, Glass Ionomer Cement, Nanocellulose |
| Pembimbing 1 | Dr. drg. A. Haris Budi Widodo, M.Kes., A.P., S.IP., S.E., S.H. |
| Pembimbing 2 | Dr. Apt. Tuti Sri Suhesti, S.Si., M.Sc |
| Pembimbing 3 | |
| Tahun | 2025 |
| Jumlah Halaman | 5 |
| Tgl. Entri | 2025-05-22 15:44:54.861758 |