| NIM | A1D021149 |
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| Namamhs | IVONNE RESTU TRIA |
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| Judul Artikel | Isolasi dan Karakterisasi Bakteri Rizosfer Padi Sawah sebagai Agen Bioremediasi Timbal |
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| Abstrak (Bhs. Indonesia) | Pencemaran timbal (Pb) akibat aktivitas industri dan pertanian berdampak negatif terhadap mikroorganisme tanah dan kesehatan manusia. Masalah ini dapat diatasi dengan menggunakan bakteri indigenus. Penelitian ini bertujuan mengkaji populasi bakteri di lahan sawah organik dan konvensional serta mengidentifikasi isolat bakteri indigenus yang resisten terhadap Pb. Sampel diambil dari rizosfer padi, dilanjutkan dengan isolasi, karakterisasi, uji resistensi, uji bioremediasi, dan pengamatan pertumbuhan bakteri. Populasi bakteri yang ditemukan di lahan sawah konvensional mencapai 3,3 × 10⁶ CFU/g, sementara di sawah organik adalah 1,2 × 10⁵ CFU/g. Isolat O5 menunjukkan kemampuan dalam mereduksi kadar timbal (Pb) hingga 87,858%, sedangkan isolat K2 mencapai 79,729%. Temuan ini mengindikasikan bahwa bakteri rizosfer memiliki potensi sebagai agen bioremediasi terhadap pencemaran Pb. |
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| Abtrak (Bhs. Inggris) | Lead (Pb) pollution due to industrial and agricultural activities has a negative impact on soil microorganisms and human health. This problem can be solved by using indigenous bacteria. This study aims to examine bacterial populations in organic and conventional rice fields and identify isolates of indigenous bacteria that are resistant to Pb. Samples were taken from rice rhizospheres, followed by isolation, characterization, resistance tests, bioremediation tests, and observation of bacterial growth. The bacterial population found in conventional rice fields reached 3.3 × 10⁶ CFU/g, while in organic rice fields it was 1.2 × 10⁵ CFU/g. O5 isolate showed the ability to reduce lead (Pb) levels by up to 87.858%, while K2 isolate reached 79.729%. These findings indicate that rhizosphere bacteria have potential as bioremediation agents against Pb pollution. |
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| Kata kunci | Pb, padi budidaya organik, padi budidaya konvensional, bioremediasi |
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| Pembimbing 1 | Sapto Nugroho Hadi, S.Si., M.Biotech. |
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| Pembimbing 2 | Prita Sari Dewi, S.P., M.Sc., Ph.D. |
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| Pembimbing 3 | |
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| Tahun | 2025 |
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| Jumlah Halaman | 14 |
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| Tgl. Entri | 2025-05-20 13:38:25.479226 |
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