| NIM | A1D021042 |
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| Namamhs | APRILIA FATMAWATI |
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| Judul Artikel | Isolasi dan Karakterisasi Bacillus sp. Endofit Asal Perakaran Putri Malu Dataran Rendah sebagai Pemacu Pertumbuhan dan Antagonis Penyakit Antraknosa Buah Cabai Rawit |
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| Abstrak (Bhs. Indonesia) | Penyakit antraknosa oleh Colletotrichum spp. merupakan salah satu penyakit penting tanaman cabai rawit, dengan kerugian mencapai 60%. Penelitian ini bertujuan mengisolasi Bacillus sp. dari akar putri malu dataran rendah, mengetahui potensinya sebagai antagonis dan Plant Growth-Promoting Bacteria Endophytes (PGPE), serta mengetahui efektivitasnya terhadap antraknosa buah cabai rawit. Metode penelitian diawali dengan eksplorasi bakteri endofit dari perakaran putri malu dataran rendah sehat, kemudian dikarakter morfologi dan biokimia. Uji antagonis dilakukan terhadap Colletotrichum spp., Pythium sp., Fusarium sp., Xanthomonas sp., dan Ralstonia sp. serta uji PGPE pada benih mentimun. Lima isolat terbaik (BE15, BE16, BE17, BE25, dan BE211) dengan kontrol dan fungisida (bahan aktif Propineb 70%) dengan empat ulangan dicoba terhadap antraknosa buah cabai rawit, menggunakan Rancangan Acak Lengkap. Variabel yang diamati karakter morfologi dan biokimia, kecepatan tumbuh benih, panjang akar, bobot segar akar dan kecambah mentimun, masa inkubasi, luas serangan, penekanan persentase penyakit, dan indeks sampah. Hasil penelitian diperoleh 22 isolat dengan karakter sel berbentuk batang, gram positif, katalase dan oksidase positif, dan memproduksi endospora. Berdasarkan uji antagonis dan PGPE, dipilih 5 isolat terbaik, yaitu BE15, BE16, BE17, BE25, dan BE211, ditunjukkan uji antagonis terhadap Pythium sp., Colletotrichum sp., dan Fusarium sp. mampu menghambat jamur patogen lebih dari 50%, sedangkan terhadap Xanthomonas sp., isolat BE15 membentuk zona bening 1,61 mm dan terhadap Ralstonia sp., isolat BE211 membentuk zona bening 3,5 mm. Sebagai PGPE, isolat BE17 dan BE33 meningkatkan panjang akar benih mentimun sebesar 1,8 dan 2,03%. Isolat BE15 dan BE25 efektif mengendalikan penyakit antraknosa buah cabai rawit dengan efektivitas masing-masing 63%. |
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| Abtrak (Bhs. Inggris) | Anthracnose caused by Colletotrichum spp. is one of the important diseases of chili pepper with losses reaching 60%. This research aimed to isolate endophytic Bacillus sp. from the lowland creeping-sensitive plants roots, to know its potential as antagonist and growth promoter, and to determine its effectiveness towards anthracnose of chili pepper fruit. The research began with exploration of endophytic bacteria, then characterized morphologically and biochemically. The antagonist test was carried out against Colletotrichum spp., Pythium sp., Fusarium sp., Xanthomonas sp., and Ralstonia sp. and growth promoter test on cucumber seeds. The five best isolates (BE15, BE16, BE17, BE25, and BE211) with control and fungicide (a.i. Propineb 70%) as treatment with four replicates used a Completely Randomized Design against the disease. Variables observed were morphological and biochemical characters, seed growth speed, root length, fresh weight of cucumber roots and sprouts, incubation period, area of attack, and waste index. Results of the research obtained 22 isolates with the characteristics of rod-shaped cells, gram positive, catalase and oxidase positive, and produced endospores. The five best selected isolates, BE15, BE16, BE17, BE25, and BE211, were able to inhibit pathogenic fungi by more than 50%, while against Xanthomonas sp., BE15 isolate formed a clear zone of 1.61 mm and against Ralstonia sp., BE211 isolate formed 3.5 mm. BE17 and BE33 isolates increased the root length of cucumber seeds by 1.8 and 2.03%. BE15 and BE25 isolates were effective in controlling the disease with an effectiveness of 63% each. |
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| Kata kunci | agensia hayati, antraknosa, Bacillus sp. endofit, cabai rawit, Mimosa invisa |
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| Pembimbing 1 | Prof. Ir. Loekas Soesanto, M.S., Ph.D. |
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| Pembimbing 2 | Prof. Dr. Ir. Tamad, M.Si. |
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| Pembimbing 3 | |
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| Tahun | 2025 |
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| Jumlah Halaman | 16 |
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| Tgl. Entri | 2025-05-15 20:54:42.445812 |
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