Artikel Ilmiah : B1A020066 a.n. MAUDY SYIFA SALSABILA
| NIM | B1A020066 |
|---|---|
| Namamhs | MAUDY SYIFA SALSABILA |
| Judul Artikel | Isolasi dan Identifikasi Fenotipik dan Genotipik Isolat Bakteri Resisten Antibiotik Beta-laktam asal Feses Tikus Konsumsi dari Sulawesi Utara |
| Abstrak (Bhs. Indonesia) | Tikus hutan menjadi salah satu satwa yang paling sering diburu untuk dikonsumsi oleh masyarakat Sulawesi Utara. Kebersihan pada saat pengolahan daging tikus perlu diperhatikan karena tikus dianggap sebagai reservoir yang membawa dan menyebarkan penyakit yang dapat menular dari hewan ke manusia (zoonosis). Infeksi mikroba patogen, seperti bakteri dan virus dapat menular ke manusia melalui kontak dengan cairan tubuh hewan pada saat proses pemotongan dan pengolahan daging serta melalui kotoran dan urin tikus yang mengandung mikroba patogen, seperti bakteri pada family Enterobacteriaceae. Infeksi bakteri Enterobacteriaceae bersifat nosokomial dan penanganannya membutuhkan antibiotik sebagai agen penghambat pertumbuhan bakteri. Ceftriaxone merupakan antibiotik golongan Cephalosporin generasi ketiga yang digunakan untuk penanganan infeksi Enterobacteriaceae. Bakteri patogenik yang berasal dari feses tikus berpotensi membawa sifat resistensi terhadap antibiotik. Oleh karena itu, tujuan dari penelitian ini adalah mengisolasi dan mengidentifikasi bakteri asal feses tikus konsumsi yang resisten antibiotik Beta-laktam. Penelitian dilakukan menggunakan metode survei dengan variabel bebas dari penelitian ini adalah jenis dan dosis antibiotik dan variabel terikat adalah respon isolat bakteri asal feses tikus terhadap antibiotik. Pengambilan sampel feses tikus di pasar tradisional di Sulawesi Utara dan dilakukan dengan metode purposive sampling. Isolasi bakteri resisten Ceftriaxone asal feses tikus dilakukan menggunakan media MacConkey Agar. Bakteri yang resisten Ceftriaxone akan dilakukan uji resistensi antibiotik atau Antimicrobial Susceptibility Test (AST) menggunakan metode Kirby-Bauer terhadap beberapa antibiotik, yaitu Ceftriaxone (CRO), Cefoxitine (FOX), Amoxycillin Clauvid Acid (AMC), dan Imipenem (IPM). Isolat bakteri diidentifikasi secara fenotipik, biokimia, dan molekuler berdasarkan gen 16S rRNA. Gen resisten Extended-spectrum Beta-lactamase, yaitu blaSHV, blaCTX-M, dan blaTEM juga diamplifikasi dari isolat bakteri yang resisten Ceftriaxone. Data hasil penelitian dianalisis secara deskriptif. Hasil penelitian menunjukkan terdapat dua siolat bakteri yang resisten terhadap Ceftriaxone, yaitu isolat 1C dan 5C. Kedua isolat tersebut juga resisten terhadap CRO, FOX, dan AMC, serta sensitif terhadap IPM pada metode AST dan terkonfirmasi memiliki gen resisten blaTEM. Berdasarkan hasil karakterisasi fenotipik, biokimiawi, dan molekuler, kedua isolat berkerabat dekat dengan Klebsiella aerogenes dengan similaritas 99,85% untuk isolat 1C dan 100% untuk isolat 5C. |
| Abtrak (Bhs. Inggris) | Forest rats are one of the most commonly hunted animals for consumption by the people of North Sulawesi. Hygiene during the processing of rat meat needs to be considered because rats are considered as reservoirs that possible carry and spread diseases from the animal to humans (zoonosis). Pathogenic microbes infections, for instance by bacteria and viruses, can be transmitted to humans through contact with animal body fluids during slaughtering and meat processing as well as through rat feces and urine containing pathogenic microbes, including bacteria from in the Enterobacteriaceae family. Enterobacteriaceae bacterial infections are nosocomial and their treatment requires antibiotics as agents that inhibit bacterial growth. Ceftriaxone is a third-generation Cephalosporin class antibiotic used for the treatment of Enterobacteriaceae infections. Pathogenic bacteria from rat fecal content may potentially harbor antibiotic resistance trait. Therefore, the purpose of this study is to isolate and identify bacteria from the feces of consumption rats that are resistant to Ceftriaxone antibiotics. The research was conducted using survey method with the independent variables of this research are the type and dose of antibiotics and the dependent variable is the response of the bacterial isolated bacteria from rat feces to antibiotics. Sampling of rat feces in traditional markets in North Sulawesi was done by purposive sampling method. Isolation of Ceftriaxone-resistant bacteria from rat feces was conducted by using MacConkey Agar media. Ceftriaxone-resistant bacteria were tested for antibiotic resistance or Antimicrobial Susceptibility Test (AST) using the Kirby-Bauer method against several antibiotics, including Ceftriaxone (CRO), Cefoxitine (FOX), Amoxycillin Clauvid Acid (AMC), and Imipenem (IPM). Bacterial isolates were identified by phenotypic, biochemical test and molecular approaches based on the 16S rRNA gene. Extended-spectrum Beta-lactamase resistant genes (blaSHV, blaCTX-M, and blaTEM) were amplified from Ceftriaxone-resistant bacterial isolates. The obtained data were analyzed by descriptive approaches. The results showed that there were two bacterial isolates that were resistant to Ceftriaxone (1C and 5C). Both isolates were resistant to CRO, FOX, and AMC, and were sensitive to IPM in the AST method and were confirmed to have a blaTEM resistant gene. Based on the results of phenotypic, biochemical, and molecular characterization, both isolates are closely related to Klebsiella aerogenes with similarity 99.85% for 1C and 100% for 5C. |
| Kata kunci | Ceftriaxone, Entrobacteriaceae, resisten, Sulawesi Utara, tikus hutan |
| Pembimbing 1 | Dr. Sri Martina Wiraswati, S.P., M.Si. |
| Pembimbing 2 | drh. Herjuno Ari Nugroho, M.Sc. |
| Pembimbing 3 | |
| Tahun | 2025 |
| Jumlah Halaman | 76 |
| Tgl. Entri | 2025-02-10 13:10:56.687745 |