| NIM | A1D020173 |
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| Namamhs | NISA HANIFAH MILLATINA |
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| Judul Artikel | ISOLASI DAN KARAKTERISASI BAKTERI RIZOSFER PADI PADA SISTEM PERTANIAN ORGANIK DAN ANORGANIK DI DESA MERDEN KECAMATAN PURWANEGARA KABUPATEN BANJARNEGARA |
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| Abstrak (Bhs. Indonesia) | Di Desa Merden terdapat dua sistem budidaya yaitu sistem pertanian organik dan sistem pertanian konvensional atau anorganik. Penelitian ini bertujuan untuk mengetahui jumlah populasi bakteri rizosfer pada lahan pertanian padi sistem organik dan anorganik, dan mengetahui potensi PGPR pada pertanian organik dan anorganik. Penelitian ini dilaksanakan pada bulan Maret 2024-Juni 2024 di Unit Mikrobiologi Laboratorium Agroekologi, Fakultas Pertanian, Universitas Jenderal Soedirman, Purwokerto. Pengambilan sampel tanah dilakukan secara komposit dengan teknik pengambilan secara diagonal. Sampel tanah yang diperoleh kemudian dilakukan isolasi bakteri menggunakan metode pour plate pada medium Nutrient Agar. Isolat yang telah diperoleh selanjutnya dilakukan karakterisasi morfologi makroskopis dan morfologi mikroskopis, serta uji potensi PGPR. Hasil penelitian menunjukkan bahwa populasi bakteri rizosfer yang berasal dari lahan pertanian organik lebih tinggi yaitu sebesar 12,34 x 108 CFU/mL dibandingkan dengan lahan anorganik yang hanya sebesar 1,91 x 108 CFU/ml. Setiap isolat bakteri memiliki morfologi yang berbeda, namun terdapat beberapa isolat yang sama dari sampel lahan pertanian organik (OG) dan lahan pertanian anorganik (ANK), yaitu OG6 dan ANK7 memiliki bentuk tidak beraturan, ukuran titik, tepi undulate, elevasi datar, dan warna putih, serta OG10, ANK1, dan ANK2 memiliki bentuk bulat, ukuran sedang, tepi entire, elevasi datar, dan warna putih. Lahan organik memiliki 1 bakteri Gram negatif dan 9 Gram positif, sedangkan lahan anorganik memiliki 2 bakteri Gram negatif dan 8 bakteri Gram positif dengan bentuk sel basil, kokus, dan spirilli. Hasil pengujian potensi PGPR menunjukkan bahwa seluruh isolat bakteri lahan organik memiliki kemampuan menambat nitrogen, sedangkan isolat bakteri lahan anorganik yang memiliki kemampuan menambat nitrogen yaitu ANK2, ANK3, ANK5, dan ANK8. Isolat yang memiliki kemampuan melarutkan fosfat yaitu OG4, OG6 dan OG9. |
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| Abtrak (Bhs. Inggris) | In Merden Village there are two cultivation systems, namely organic farming systems and conventional or inorganic farming systems. This research aims to determine the population of rhizosphere bacteria in organic and inorganic rice farming systems, and determine the potential of PGPR in organic and inorganic farming. This research was carried out in March 2024-June 2024 at the Microbiology Unit of the Agroecology Laboratory, Faculty of Agriculture, Jenderal Soedirman University, Purwokerto. Soil samples were taken compositely using a diagonal sampling technique. The soil samples obtained were then isolated for bacteria using the pour plate method on Nutrient Agar medium. The isolates that were obtained were then characterized by macroscopic morphology and microscopic morphology, as well as testing for PGPR potential. The research results showed that the population of rhizosphere bacteria originating from organic agricultural land was higher, namely 12.34 x 108 CFU/mL compared to inorganic land which was only 1.91 x 108 CFU/ml. Each bacterial isolate has a different morphology, but there are several isolates that are the same from organic agricultural land (OG) and inorganic agricultural land (ANK) samples, namely OG6 and ANK7 have irregular shapes, point sizes, undulate edges, flat elevations, and colors. white, as well as OG10, ANK1, and ANK2 have a round shape, medium size, entire edge, flat elevation, and white color. Organic land has 1 Gram-negative and 9 Gram-positive bacteria, while inorganic land has 2 Gram-negative bacteria and 8 Gram-positive bacteria in the form of bacilli, cocci and spirilli cells. The results of PGPR potential testing showed that all organic land bacterial isolates had the ability to fix nitrogen, while the inorganic land bacterial isolates that had the ability to fix nitrogen were ANK2, ANK3, ANK5, and ANK8. Isolates that have the ability to dissolve phosphate are OG4, OG6 and OG9. |
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| Kata kunci | Kata kunci: organik, anorganik, bakteri |
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| Pembimbing 1 | Ida Widiyawati, S.P., M.Si. |
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| Pembimbing 2 | Ratri Noorhidayah, S.P., M.Sc. |
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| Pembimbing 3 | |
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| Tahun | 2025 |
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| Jumlah Halaman | 12 |
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| Tgl. Entri | 2025-02-07 12:55:28.943491 |
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