| NIM | G1A021067 |
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| Namamhs | AULIA ADY NUGRAHA |
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| Judul Artikel | UJI AKTIVITAS ANTIJAMUR EKSTRAK KAYU MANIS (Cinnamomum burmannii) TERHADAP ISOLAT Aspergillus niger DARI PASIEN OTOMIKOSIS SECARA IN VITRO |
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| Abstrak (Bhs. Indonesia) | Latar Belakang: Otomikosis merupakan infeksi jamur superfisial pada canalis auditorius externus yang sering disebabkan oleh Aspergillus niger. Resistensi antifungal terhadap A. niger semakin meningkat sehingga diperlukan alternatif terapi untuk pengobatan otomikosis akibat A. niger. Kayu manis (Cinnamomum burmannii) diketahui memiliki senyawa aktif sinamaldehid, flavonoid, alkaloid, saponin, dan tanin yang diduga memiliki aktivitas antijamur. Tujuan: Mengetahui aktivitas antijamur ekstrak kayu manis (C. burmannii) pada konsentrasi 1,5%, 2%, 4%, dan 8% terhadap pertumbuhan A. niger dari pasien otomikosis secara in vitro. Metode: Penelitian ini menggunakan metode true experimental post test only control group design. Ekstrak C. burmannii diperoleh melalui metode maserasi menggunakan pelarut etanol 96%. Uji aktivitas antijamur dilakukan dengan metode well diffusion dengan mengukur diameter zona hambat. Variabel bebas penelitian ini yaitu ekstrak C. burmannii konsentrasi 1,5%, 2%, 4%, dan 8%. Kontrol negatif berupa aquadest steril, kontrol pelarut DMSO 10%, dan kontrol positif itraconazole (8 µg/ml). Hasil pengukuran dianalisis secara deskriptif dan analisis statistik menggunakan SPSS. Hasil: Hasil penelitian menunjukkan ekstrak C. burmannii 1,5%, 2%, 4%, dan 8% tidak membentuk zona hambat di sekitar sumuran, menandakan tidak adanya penghambatan terhadap pertumbuhan A. niger. Hasil ini dikarenakan kemungkinan konsentrasi senyawa fitokimia C. burmannii tidak cukup besar untuk menghambat pertumbuhan A. niger dari isolat pasien otomikosis. Kesimpulan: Ekstrak C. burmannii 1,5%, 2%, 4%, dan 8% tidak menunjukkan aktivitas antijamur terhadap A. niger dari isolat pasien otomikosis. |
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| Abtrak (Bhs. Inggris) | Background: Otomycosis is a superficial fungal infection of the external auditory canal predominantly caused by Aspergillus niger. The increasing antifungal resistance against A. niger necessitates alternative therapeutic approaches for treating A. niger-induced otomycosis. Cinnamon (Cinnamomum burmannii) contains active compounds including cinnamaldehyde, flavonoids, alkaloids, saponins, and tannins that are hypothesized to possess antifungal activity. Objective: To evaluate the in vitro antifungal activity of cinnamon (C. burmannii) extract at concentrations of 1.5%, 2%, 4%, and 8% against A. niger isolated from otomycosis patients. Method: This research employed a true experimental posttest-only control group design. C. burmannii extract was obtained through maceration using 96% ethanol as solvent. The antifungal activity test was conducted using the well diffusion method by measuring the inhibition zone diameter. The independent variable was C. burmannii extract at concentrations of 1.5%, 2%, 4%, and 8%. Sterile distilled water served as negative control, 10% DMSO as solvent control, and itraconazole (8 µg/ml) as positive control. The measurement results were analyzed using descriptive and statistical analysis with SPSS. Results: The study revealed that C. burmannii extract at concentrations of 1.5%, 2%, 4%, and 8% produced no inhibition zones around the wells, indicating absence of growth inhibition against A. niger. This outcome may be attributed to insufficient concentrations of C. burmannii phytochemical compounds required to inhibit the growth of A. niger isolated from otomycosis patients. Conclusion: C. burmannii extract at concentrations of 1.5%, 2%, 4%, and 8% demonstrated no antifungal activity against A. niger isolated from otomycosis patients. |
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| Kata kunci | Antijamur Aspergillus niger, Cinnamomum burmannii, Kayu Manis, Otomikosis |
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| Pembimbing 1 | Anriani Puspita Karunia Ning Widhi |
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| Pembimbing 2 | Nia Krisniawati |
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| Pembimbing 3 | |
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| Tahun | 2025 |
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| Jumlah Halaman | 12 |
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| Tgl. Entri | 2025-01-21 15:26:49.075862 |
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