| NIM | B1A020111 |
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| Namamhs | TYAS WANDA SEPTIANI |
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| Judul Artikel | Eksplorasi Fungi Rizosfer Perqakaran Tanaman Damar (Agathis dammatra) Kebun Raya Baturraden Sebagai Agen Biokontrol Fusarium oxysporum Secara In Vitro. |
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| Abstrak (Bhs. Indonesia) | Fusarium oxysporum merupakan patogen penyebab layu batang pada beberapa tumbuhan. Salah satu solusi pengendalian yang dapat dilakukan yakni dengan memanfaatkan mikroorganisme yang bersifat antagonis terhadap patogen seperti fungi antagonis Penelitian ini bertujuan untuk mengetahui isolat fungi apa yang didapatkan dari hasil isolasi tanah rizosfer tanaman Agathis dammara di Kebun Raya Baturraden dan mengetahui bagaimana kemampuan fungi tersebut dalam menghambat pertumbuhan jamur patogen F. oxysporum. Penelitian ini dilakukan menggunakan dua metode yakni metode survey dan metode eksperimental. Metode survey digunakan pada saat sampling dengan teknik purposive random sampling, dan metode eksperimental dilakukan pada uji antagonis. Isolat yang didapat dari hasil isolasi diuji antagonis dengan patogen F. oxysporum secara in-vitro menggunakan metode dual culture dan metode non-volatil. Variabel yang digunakan untuk uji antagonis yakni persentase daya hambat fungi hasil isolasi dengan patogen tanaman F. oxysporum. Parameter yang diamati berupa diameter fungi F. oxysporum, persentase daya hambat, dan mekanisme yang terjadi. Hasil persentase daya hambat uji antagonis dual culture dan non-volatil dianalisis menggunakan Analysis of Variance (ANOVA). Kemudian hasil ANOVA dianalisis menggunakan uji lanjut Duncan Multiple Range Test (DMRT) pada taraf kepercayaan 95%. Karakterisasi yang mengacu pada buku Compendium of Soil Fungi menununjukkan 5 dari 7 isolat hasil isolasi rizosfer Agathis dammara di Kebun Raya Baturraden teridentifikasi sebagai Penicillium sp., Trichoderma sp., dan Aspergillus sp.. Hasil uji dual culture dan non volatil menunjukkan jamur hasil isolasi bersifat antagonis terhadap jamur patogen Fusarium sp. dengan rata-rata persentase hambat berturut-turut yaitu 41,74%-90,07% dan 78,18%-89,38%.
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| Abtrak (Bhs. Inggris) | Fusarium oxysporum is a pathogen that causes stem wilt in several plants. One control solution that can be done is by utilizing microorganisms that are antagonistic to pathogens such as antagonistic fungi. This study aims to determine what fungal isolates are obtained from the results of rhizosphere soil isolation of Agathis dammara plants in the Baturraden Botanical Gardens and to determine the ability of these fungi to inhibit the growth of the pathogenic fungus F. oxysporum. This study was conducted using two methods, namely the survey method and the experimental method. The survey method was used during sampling with the purposive random sampling technique, and the experimental method was carried out in the antagonist test. The isolates obtained from the isolation results were tested antagonistically with the pathogen F. oxysporum in vitro using the dual culture method and the non-volatile method. The variables used for the antagonist test were the percentage of the inhibitory power of the isolated fungi with the plant pathogen F. oxysporum. The parameters observed were the diameter of the F. oxysporum fungus, the percentage of inhibition, and the mechanisms that occurred. The results of the percentage of inhibition of the dual culture and non-volatile antagonist tests were analyzed using Analysis of Variance (ANOVA). Then the ANOVA results were analyzed using the Duncan Multiple Range Test (DMRT) at a 95% confidence level. Characterization referring to the Compendium of Soil Fungi book showed that 5 out of 7 isolates from the rhizosphere of Agathis dammara in the Baturraden Botanical Garden were identified as Penicillium sp., Trichoderma sp., and Aspergillus sp. The results of the dual culture and non-volatile tests showed that the isolated fungi were antagonistic to the pathogenic fungus Fusarium sp. with an average percentage of inhibition of 41.74% -90.07% and 78.18% -89.38% respectively. |
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| Kata kunci | Eksplorasi, fungi antagonis, Fusarium oxysporum, non-volatil, rizosfer |
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| Pembimbing 1 | Prof. Dr. Nuraeni Ekowati M.S. |
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| Pembimbing 2 | Dr. Juni Safitri Muljowati S. Si., M. Si. |
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| Pembimbing 3 | |
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| Tahun | 2024 |
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| Jumlah Halaman | 15 |
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| Tgl. Entri | 2024-11-20 20:51:43.342148 |
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