| NIM | B1A020012 |
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| Namamhs | ANINDYA PUSPANING TYAS |
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| Judul Artikel | Upaya Memacu Pembentukan Kantong pada Nepenthes mirabilis (Lour.) Druce secara In Vitro melalui Manipulasi Media dan Penambahan BAP |
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| Abstrak (Bhs. Indonesia) | Penelitian ini dilakukan dengan tujuan untuk mengetahui pengaruh interaksi antara formulasi media dan konsentrasi BAP pada pembentukan kantong N. mirabilis secara in vitro serta menentukan formulasi media dan konsentrasi BAP yang paling baik untuk memacu pembentukan kantong pada N. mirabilis secara in vitro. Penelitian ini dilaksanakan secara eksperimental dengan Rancangan Acak Lengkap (RAL) pola perlakuan faktorial. Faktor pertama adalah formulasi media yang terdiri atas Full-Strength MS, 3/4-Strength MS, 2/4-Strength MS, dan 1/4-Strength MS. Faktor kedua adalah konsentrasi BAP yang terdiri atas 0 μM; 0,5 μM; 1,0 μM; dan 1,5 μM. Data yang diperoleh dianalisis dengan Analysis of Variance (ANOVA) dengan probabilitas 5% dan 1% dan dilanjutkan dengan uji jarak berganda Duncan dengan tingkat kesalahan 5%. Hasil penelitian menunjukkan bahwa interaksi antara formulasi media dan konsentrasi BAP berpengaruh terhadap pertambahan jumlah daun, jumlah kantong yang terbentuk, dan persentase daun yang membentuk kantong pada N. mirabilis dalam kultur in vitro. Sementara pertambahan jumlah tunas dipengaruhi oleh faktor mandiri formulasi media dan konsentrasi BAP yang digunakan. Media 1/4-Strength MS dengan penambahan 0,5 μM BAP merupakan media terbaik untuk memacu pembentukan kantong pada N. mirabilis dalam kultur in vitro. |
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| Abtrak (Bhs. Inggris) | This research has been conducted with a view to study the effect of the interaction between media formulation and BAP concentration on in vitro pitcher formation of N. mirabilis, as well as to determine the best media formulation and BAP concentration to induce in vitro pitcher formation of N. mirabilis. This study has been carried out experimentally using a completely randomized design on a factorial treatment pattern. The first factor was the media formulations, which consisted of: Full-Strength MS, 3/4-Strength MS, 2/4-Strength MS, and 1/4-Strength MS. The second factor was the BAP concentrations consisted of: 0 μM; 0,5 μM; 1,0 μM; and 1,5 μM. The data obtained were analyzed using an Analysis of Variance (ANOVA) at 5% and 1% probability levels, followed by Duncan's Multiple Range Test at a 5% error level. The results showed that the interaction between media formulation and BAP concentration controlled the increase in the number of leaves, number of pitchers, and the percentage of leaves forming pitchers of N. mirabilis. The number of shoots formed was determined by sole factor of media formulations and BAP concentrations. In addition, the pitcher emergence time was controlled by BAP concentration. The 1/4-Strength MS supplemented with 0,5 μM BAP was the best media for in vitro pitcher formation of N. mirabilis. The research results showed that the interaction between media formulations and BAP concentration controlled leaf formation, number of pitchers formed and the percentage of pitcher forming leaves. Meanwhile, shoot formation was controlled by sole media formulations and BAP concentrations used. A 1/4-Strength MS supplemented with 0,5 μM BAP resulted in the best pitcher formation of N. mirabilis in in vitro culture. |
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| Kata kunci | BAP; in vitro; kantong; MS; Nepenthes mirabilis. |
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| Pembimbing 1 | Sugiyono, Ph.D. |
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| Pembimbing 2 | Sri Lestari, S.Si., M.Si. |
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| Pembimbing 3 | |
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| Tahun | 2024 |
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| Jumlah Halaman | 15 |
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| Tgl. Entri | 2024-10-11 15:18:05.561454 |
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