Artikel Ilmiah : B1B018041 a.n. FURIDIAN KHAIRUNISSA
| NIM | B1B018041 |
|---|---|
| Namamhs | FURIDIAN KHAIRUNISSA |
| Judul Artikel | Some Morphological and Physiological Characters of Vanda bensonii Bateman Seedlings under Colchicine Application”. |
| Abstrak (Bhs. Indonesia) | Vanda bensonii is an orchid species having potentials to be developed. On the other hands, still only few studies on Vanda orchids, especially in Indonesia, have been reported. One of the efforts to develop the potentials of Vanda orchids including V. bensonii is to increase the genetic variation by means of induced mutation, which may result in polyploid individuals. Such individuals usually exhibit better phenotypical performance than that of the normal diploids. This can be performed by the application of antimitotic chemicals such as colchicine. Therefore, this study aims to know the effect of colchicine on some morphological and physiological characters of V. bensonii seedlings and to find out the effective concentration in producing the most promising ideal phenotypical performance of the orchid species. The study was conducted at the Plant Physiology Laboratory, the Faculty of Biology, Universitas Jenderal Soedirman from October 2022 to May 2023. An experimental method was employed and arranged in a Completely Randomized Design with treatments consisting of five colchicine levels of concentrations, i.e. 0, 20, 40, 60, and 80 µM. All treatments were subjected to five replications giving rise to 25 experimental units. The independent variable was colchicine concentration, while the dependent variables comprised morphological and physiological characters. The morphological parameters comprised leaf colour, leaf length and width, and root number, while the physiological parameters consisted of total chlorophyll, chlorophyll a and b. The data obtained were analyzed using ANOVA with an F test of 0.05 and 0.01. Further analysis using Tukey test of 0.05 was performed when significant differences between treatments. The results show that colchicine significantly reduces plant height of V. bensonii, in which higher concentrations of colchicine cause shorter V. bensonii. Similarly, it is also the case with chlorophyll a content, while there is no significant effect of colchicine on leaf width, leaf length, leaf number, shoot number, chlorophyll b and total chlorophyll contents. Nevertheless, colchicine concentration of 60 µM is found most effective in increasing shoot number. It can be concluded that colchicine has significant effects on plant height and chlorophyll a content of V. bensonii. |
| Abtrak (Bhs. Inggris) | Vanda bensonii is an orchid species having potentials to be developed. On the other hands, still only few studies on Vanda orchids, especially in Indonesia, have been reported. One of the efforts to develop the potentials of Vanda orchids including V. bensonii is to increase the genetic variation by means of induced mutation, which may result in polyploid individuals. Such individuals usually exhibit better phenotypical performance than that of the normal diploids. This can be performed by the application of antimitotic chemicals such as colchicine. Therefore, this study aims to know the effect of colchicine on some morphological and physiological characters of V. bensonii seedlings and to find out the effective concentration in producing the most promising ideal phenotypical performance of the orchid species. The study was conducted at the Plant Physiology Laboratory, the Faculty of Biology, Universitas Jenderal Soedirman from October 2022 to May 2023. An experimental method was employed and arranged in a Completely Randomized Design with treatments consisting of five colchicine levels of concentrations, i.e. 0, 20, 40, 60, and 80 µM. All treatments were subjected to five replications giving rise to 25 experimental units. The independent variable was colchicine concentration, while the dependent variables comprised morphological and physiological characters. The morphological parameters comprised leaf colour, leaf length and width, and root number, while the physiological parameters consisted of total chlorophyll, chlorophyll a and b. The data obtained were analyzed using ANOVA with an F test of 0.05 and 0.01. Further analysis using Tukey test of 0.05 was performed when significant differences between treatments. The results show that colchicine significantly reduces plant height of V. bensonii, in which higher concentrations of colchicine cause shorter V. bensonii. Similarly, it is also the case with chlorophyll a content, while there is no significant effect of colchicine on leaf width, leaf length, leaf number, shoot number, chlorophyll b and total chlorophyll contents. Nevertheless, colchicine concentration of 60 µM is found most effective in increasing shoot number. It can be concluded that colchicine has significant effects on plant height and chlorophyll a content of V. bensonii. |
| Kata kunci | colchicine, morphological characters, physiological characters, polyploid, Vanda bensonii |
| Pembimbing 1 | Prof. Dr. Murni Dwiati., M.si |
| Pembimbing 2 | Prof.Dr.Agus Hery Susanto.,M.S |
| Pembimbing 3 | |
| Tahun | 2024 |
| Jumlah Halaman | 38 |
| Tgl. Entri | 2024-08-01 16:33:26.666924 |