Artikel Ilmiah : G1A009020 a.n. Dera Fakhrunnisa
| NIM | G1A009020 |
|---|---|
| Namamhs | Dera Fakhrunnisa |
| Judul Artikel | Perbedaan Kadar Testosteron Serum Tikus Putih (Rattus norvegicus) Jantan pada Model Stres Paradoxical Sleep Deprivation, Imobilisasi, dan Footshock Kronik |
| Abstrak (Bhs. Indonesia) | Latar Belakang: Testosteron merupakan hormon yang paling penting dalam sistem reproduksi pria. Pada usia sekitar 30 tahun kadar testosteron mulai menurun dan terus menurun secara bertahap sekitar 1% per tahun. Penurunan kadar testosteron dapat dipercepat dengan adanya stres. Tujuan: Tujuan penelitian ini adalah mengetahui perbedaan kadar testosteron serum tikus putih (Rattus norvegicus) jantan pada model stres PSD, imobilisasi dan footshock kronik. Metode: Penelitian ini menggunakan 21 ekor tikus putih jantan (Rattus norvegicus) galur wistar usia 3-4 bulan, berat badan 200-300 gram. Tikus dibagi 4 kelompok, yaitu: kelompok 1 sebagai kontrol tanpa perlakuan, kelompok 2 diberikan perlakuan stres PSD, kelompok 3 diberikan perlakuan stres imobilisasi, dan kelompok 4 diberikan perlakuan stres footshock. Penelitian berlangsung selama 25 hari. Testosteron serum diperiksa 1 kali pada akhir penelitian dan diukur dengan metode Enzyme Linked Immunosorbent Assay (ELISA). Perbedaan kadar testosteron serum dianalisis menggunakan uji Analysis of Variance (ANOVA) dengan Post Hoc LSD. Hasil: Hasil penelitian menunjukkan bahwa terdapat perbedaan rerata kadar testosteron serum yang bermakna antara kelompok kontrol (0,26±0,22 ng/mL) dengan kelompok PSD kronik (0,07±0,08 ng/mL) (p=0,014) dan antara kelompok PSD kronik dengan kelompok footshock kronik(0,20±0,07 ng/mL) (p=0,011). Kesimpulan: Terdapat perbedaan rerata kadar testosteron serum tikus putih (Rattus norvegicus) jantan pada model stress PSD, imobilisasi dan footshock kronik. Kata kunci: testosteron, paradoxical sleep deprivation, imobilisasi, footshock |
| Abtrak (Bhs. Inggris) | Background: Testosteron was the most important hormone of male reproductive system. Age around 30 years old would start to do the decreasing of testosteron levels about 1% per year. Decreasing of testosteron levels could be accelerated by stress. Objective: This study aimed to know the difference of testosteron levels of male albino rats (Rattus norvegicus) with chronic PSD, immobilization, and footshock stress models. Method: This study used 21 rats (Rattus norvegicus) wistar strain 3-4 months old, 200-300 gram weight. Rats divided to 4 gorups, which was: group 1 as a control, group 2 was given PSD stress model intervention, group 3 was given immobilization stress model intervention, and group 4 was given footshock stress model intervention. The study was done for 25 days. Testosteron serum measured once at the end of the study and measured using Enzyme Linked Immunosorbent Assay (ELISA) method. The difference of testosteron serum levels analized by Analysis of Variance (ANOVA) test and followed by Post Hoc LSD. Result: The result of this study showed that there was difference of average testosteron serum levels between the control group (0,26±0,22 ng/mL) and PSD group (0,07±0,08 ng/mL) (p=0,014) and between PSD group and footshock group (0,20±0,07 ng/mL) (p=0,011). Conclusion: There was difference of average testosteron levels of male albino rats (Rattus norvegicus) with chronic PSD, immobilization, and footshock stress models. Keywords: testosteron, paradoxical sleep deprivation, immobilization, footshock |
| Kata kunci | testosteron, paradoxical sleep deprivation, imobilisasi, footshock |
| Pembimbing 1 | dr. Mustofa, M.Sc. |
| Pembimbing 2 | dr. Yudhi Wibowo, M.PH. |
| Pembimbing 3 | |
| Tahun | 2013 |
| Jumlah Halaman | 12 |
| Tgl. Entri | (belum diset) |