| NIM | B1B019010 |
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| Namamhs | HARRI RACHMAN |
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| Judul Artikel | GENETIC DIFFERENCES AMONG SEVERAL SOYBEAN VARIETIES BASED ON SCAR MARKER QS08064 |
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| Abstrak (Bhs. Indonesia) | Soybean (Glycine max, L. Merrill) is a plant species that is widely distributed in tropical and subtropical regions. Although there are very high morphological similarities among soybeans from various places, their wide distribution can affect their genetic diversity. The soybean genetic diversity can be analyzed using certain molecular markers. One of them is the SCAR (Sequence Characterized Amplified Region) molecular marker QS08064. The results of soybean genetic diversity studies can be used as a basis for developing strategies for cultivating and utilizing soybeans. This study aims to (1) identify and determine the sequences of QS08064 SCAR markers on Anjasmoro, Biosoy 1, Dega 1, and Grobogan soybean varieties, and (2) determine the type of nucleotide variations of QS08064 SCAR marker sequences among Anjasmoro, Biosoy 1, Dega 1, and Grobogan soybean varieties. Soybean sampling was carried out in the greenhouse of Biology Faculty, Jenderal Soedirman University. The working procedure consisted of genomic DNA extraction, PCR and DNA sequencing. Data on QS08064 SCAR sequences were analyzed using DnaSP software to obtain genotype diversity (g) and nucleotide diversity (π) values, as well as to obtain phylogenetic relationships among individuals. The results showed that the soybeans tested had high genetic diversity with respect to a genotype diversity value (g) of 1 and nucleotide diversity (π) of 0.01146. This explained that the soybeans tested had a close genetic relationship and a low genetic distance. |
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| Abtrak (Bhs. Inggris) | Soybean (Glycine max, L. Merrill) is a plant species that is widely distributed in tropical and subtropical regions. Although there are very high morphological similarities among soybeans from various places, their wide distribution can affect their genetic diversity. The soybean genetic diversity can be analyzed using certain molecular markers. One of them is the SCAR (Sequence Characterized Amplified Region) molecular marker QS08064. The results of soybean genetic diversity studies can be used as a basis for developing strategies for cultivating and utilizing soybeans. This study aims to (1) identify and determine the sequences of QS08064 SCAR markers on Anjasmoro, Biosoy 1, Dega 1, and Grobogan soybean varieties, and (2) determine the type of nucleotide variations of QS08064 SCAR marker sequences among Anjasmoro, Biosoy 1, Dega 1, and Grobogan soybean varieties. Soybean sampling was carried out in the greenhouse of Biology Faculty, Jenderal Soedirman University. The working procedure consisted of genomic DNA extraction, PCR and DNA sequencing. Data on QS08064 SCAR sequences were analyzed using DnaSP software to obtain genotype diversity (g) and nucleotide diversity (π) values, as well as to obtain phylogenetic relationships among individuals. The results showed that the soybeans tested had high genetic diversity with respect to a genotype diversity value (g) of 1 and nucleotide diversity (π) of 0.01146. This explained that the soybeans tested had a close genetic relationship and a low genetic distance. |
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| Kata kunci | DNA sequencing, salinity resistance, soybean, QS08064 marker |
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| Pembimbing 1 | Ir. Alice Yuniaty, M.Sc. Ph.D |
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| Pembimbing 2 | Prof. Dr. Agus Hery Susanto, M.S. |
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| Pembimbing 3 | |
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| Tahun | 2023 |
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| Jumlah Halaman | 40 |
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| Tgl. Entri | 2023-11-20 11:47:59.921716 |
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