| NIM | B1B019025 |
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| Namamhs | NIKEN LARAS DWI SAPUTRI |
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| Judul Artikel | DETECTION OF QS08064 SCAR MARKER IN SOYBEAN VAR. DEMAS 1, SLAMET, MALIKA AND DEJA 2 |
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| Abstrak (Bhs. Indonesia) | Soybean (Glycine max (L) Merr.) production in Indonesia has been decreasing every year. The existence of land conversion makes the use of marginal land as agricultural land. One of the marginal land that is widely used by farmers is saline land. Efforts to obtain soybean plants that are tolerant to salinity can be carried out through molecular tests using certain genetic markers. One of the genetic markers that can be used is the QS08064 SCAR marker. The aim of this study was to evaluate the presence of the SCAR marker QS08064 on four soybean varieties i.e Demas 1, Slamet, Malika and Deja 2, to determine the physiological response of on four soybean varieties on salinity conditions, and knowing which varieties are salinity tolerant based on phenotypic data. Experimental research was carried out using a split plot design. While the exploratory research was the detection of the QS08064 SCAR marker on the tested variety which was carried out by PCR technique using a pair of specific primers. The results of this study showed that Soybean var. Demas 1, Slamet, Malika, and Deja 2 do not contain the SCAR QS08064 marker. The four varieties tested experienced a decrease in plant height, stem diameter, number of leaves and seed weight under saline conditions. Among four varieties tested in this study, Malika has found to have salinity in seed weight lost to Grobogan. |
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| Abtrak (Bhs. Inggris) | Soybean (Glycine max (L) Merr.) production in Indonesia has been decreasing every year. The existence of land conversion makes the use of marginal land as agricultural land. One of the marginal land that is widely used by farmers is saline land. Efforts to obtain soybean plants that are tolerant to salinity can be carried out through molecular tests using certain genetic markers. One of the genetic markers that can be used is the QS08064 SCAR marker. The aim of this study was to evaluate the presence of the SCAR marker QS08064 on four soybean varieties i.e Demas 1, Slamet, Malika and Deja 2, to determine the physiological response of on four soybean varieties on salinity conditions, and knowing which varieties are salinity tolerant based on phenotypic data. Experimental research was carried out using a split plot design. While the exploratory research was the detection of the QS08064 SCAR marker on the tested variety which was carried out by PCR technique using a pair of specific primers. The results of this study showed that Soybean var. Demas 1, Slamet, Malika, and Deja 2 do not contain the SCAR QS08064 marker. The four varieties tested experienced a decrease in plant height, stem diameter, number of leaves and seed weight under saline conditions. Among four varieties tested in this study, Malika has found to have salinity in seed weight lost to Grobogan. |
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| Kata kunci | marker assisted selection, salinity, SCAR QS08064, soybean |
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| Pembimbing 1 | Ir. Alice Yuniaty, M.Sc., Ph.D |
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| Pembimbing 2 | Dr. Elly Proklamasiningsih, M.P. |
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| Pembimbing 3 | |
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| Tahun | 2023 |
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| Jumlah Halaman | 9 |
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| Tgl. Entri | 2023-08-03 00:12:33.798157 |
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