Artikel Ilmiah : G1A019086 a.n. RAFLY SYAHRIZAL YUSAN
| NIM | G1A019086 |
|---|---|
| Namamhs | RAFLY SYAHRIZAL YUSAN |
| Judul Artikel | EFEK EKSTRAK ETANOL CIPLUKAN (Physalis angulata L.) TERHADAP EKSPRESI GEN TLR-4 PADA TIKUS PUTIH (Rattus norvegicus) MODEL DIABETES MELITUS INDUKSI STZ-NA |
| Abstrak (Bhs. Indonesia) | Diabetes melitus (DM) merupakan kumpulan kelainan metabolik kompleks dengan karakteristik adanya kondisi hiperglikemia. DM dapat menimbulkan komplikasi mikrovaskular dan makrovaskular. Progresi komplikasi DM timbul karena adanya proses inflamasi berkepanjangan. Inflamasi pada DM melibatkan aktivasi Toll-Like Receptor 4 (TLR-4). Ciplukan (Physalis angulata L.) telah diteliti memiliki efek farmakologis memiliki aktivitas antiinfamasi, imunomodulasi, antiproliferasi, antioksidan, dan antibakterial. Penelitian ini dilakukan untuk mengetahui efek pemberian ekstrak etanol ciplukan (Physalis angulata L.) terhadap penurunan ekspresi gen TLR-4 pada tikus putih model DM induksi STZ-NA. Penelitian ini merupakan penelitian true experimental dengan pendekatan posttest only with control group design menggunakan bahan biologi tersimpan berupa organ pankreas tikus putih. Tikus putih berjumlah 25 ekor yang dibagi kedalam 5 kelompok berupa kelompok A: kontrol normal. B: kontrol sakit, C, D, dan E: intervensi dengan dosis 75, 150, 300 mg/KgBB ekstrak etanol ciplukan. Ekspresi gen TLR-4 diperiksa dengan metode Polymerase Chain Reaction (PCR) dan dikuantifikasi dengan software ImageJ. Analisis data menggunakan uji One Way ANOVA Welch. Didapatkan rerata ekspresi gen TLR-4 setiap kelompok yaitu A=0,81±0,41; B=1,08±0,73; C=0,42±0,18; D=1,27±0,91; E=0,34±0,20. Rerata ekspresi gen TLR-4 kelompok B lebih tinggi dari kelompok A. Terdapat tren penurunan rerata ekspresi gen TLR-4 antara kelompok B dengan C dan E. Hasil One Way ANOVA Welch menunjukkan p=0,109 sehingga tidak terdapat perbedaan rerata ekspresi gen TLR-4 yang signifikan antar seluruh kelompok. Ekstrak etanol ciplukan (Physalis angulat L.) tidak signifikan menurunkan ekspresi gen TLR-4 pada tikus putih model DM induksi STZ-NA. |
| Abtrak (Bhs. Inggris) | Diabetes mellitus (DM) is a group of complex metabolic disorder with characteristic hyperglicemia. DM causes complications such as microvascular and macrovascular complications. Progression of these complications occur due to prolonged inflammation in DM. Toll-Like Receptor 4 (TLR-4) is involved in DM prolonged inflammation process. Ciplukan (Physalis angulata L.) has been studied and its pharmacological properties such as antiinflammation, immunomodulation, antiproliferation, antioxidant, and antibacterial properties are known. This study was aimed to determine the effect of ethanolic ciplukan extract to TLR-4 gene expression decrease in diabetic STZ-NA induced white rats. This was a true experimental study with posttest only with control group design approach using stored biological material obtained in the form of pancreas from 25 white rats which were divided into 5 group. These groups were comprised as Group A: normal control, B: negative control, C, D, and E: Intervention with ethanolic ciplukan extract was done with doses as 75,150,300 mg/KgBW. Gene expression was examined using Polymerase Chain Reaction (PCR) method and quantified using ImageJ software. Data analysis was carried using One Way ANOVA Welch test. The average of TLR-4 expression in each group is A=0,81±0,41; B=1,08±0,73; C=0,42±0,18; D=1,27±0,91; E=0,34±0,20. The average for NF-κB gene expression of Group B is higher compared to group A. The result shows a decrease of the mean with group C and E compared to group B. One Way ANOVA Welch obtained p=0,109 meaning there is no significant TLR-4 average expression difference between all groups. Ethanolic extract of ciplukan put on insignificant decrease on the gene expression of TLR-4 in diabetic STZ-NA induced white rat. |
| Kata kunci | Physalis angulata, Rattus norvegicus, STZ-NA, TLR-4 |
| Pembimbing 1 | dr. Alfi Muntafiah, M.Sc |
| Pembimbing 2 | Nor Sri Inayati, S. Si., M. Biotech |
| Pembimbing 3 | |
| Tahun | 2023 |
| Jumlah Halaman | 12 |
| Tgl. Entri | 2023-07-13 19:44:21.590653 |