Artikel Ilmiah : A1D018069 a.n. SHOFIYATUN KHASANAH
| NIM | A1D018069 |
|---|---|
| Namamhs | SHOFIYATUN KHASANAH |
| Judul Artikel | Eksplorasi dan Uji Potensi Jamur sebagai Remediator Logam Berat Kadmium (Cd) dari Lahan Pertanaman Kentang di Kabupaten Brebes |
| Abstrak (Bhs. Indonesia) | Kentang (Solanum tuberosum L.) merupakan tanaman hortikultura yang menjadi bahan pangan alternatif di Indonesia. Penggunaan pestisida dan pupuk kimia oleh petani kentang sangat intensif, sehingga mengakibatkan terendapnya logam berat kadmium dalam tanah, air, maupun tanaman. Adanya pencemaran kadmium yang sukar didegradasi dan merugikan lingkungan serta makhluk hidup, maka perlu dilakukan bioremediasi. Penelitian ini bertujuan untuk mengeksplorasi, mengidentifikasi dan menguji kemampuan isolat jamur asal lahan pertanaman kentang di Kabupaten Brebes, serta menguji perkecambahan padi pada media bekas remediasi. Penelitian ini dilaksanakan di Laboratorium Mikologi Perlindungan Tanaman, Fakultas Pertanian, Universitas Jenderal Soedirman. Penelitian dilaksanakan pada bulan Januari sampai Oktober 2022. Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan satu faktor yaitu tiga isolat jamur dengan konsentrasi kadmium (0, 25 dan 50 ppm). Hasil penelitian menunjukkan bahwa terdapat 13 isolat yang ditemukan dan telah diidentifikasi dari genus Trichoderma sp., Aspergillus sp., Fusarium sp., dan Penicillium sp., serta satu isolat yang belum diidentifikasi. Tiga isolat terbaik dari hasil uji toleransi kadmium yaitu isolat dengan kode PP4 (Trichoderma sp.), WS4 (Fusarium sp.), dan WS8 (Aspergillus sp.). Isolat Trichoderma sp. mampu meremoval logam berat dalam media konsentrasi Cd 25 ppm mencapai 92% dengan kapasitas biomassa 22,75 mg/g. Biomassa terbaik pada uji kemampuan remediasi yaitu pada isolat WS4 (Fusarium sp.) dengan kadar Cd 25 ppm yaitu 0,69 g. Hasil uji perkecambahan benih padi menunjukkan semua perlakuan tidak berbeda nyata pada pengamatan bobot basah, tinggi tanaman, jumlah akar, panjang akar, dan daya perkecambahan. |
| Abtrak (Bhs. Inggris) | Potato (Solanum tuberosum L.) is a horticultural crop which is an alternative food in Indonesia. The use of pesticides and chemical fertilizers by potato farmers is very intensive, resulting in the deposition of the heavy metal cadmium in soil, water and plants. The existence of cadmium pollution which is difficult to degrade and harms the environment and living things, it is necessary to do bioremediation. This study aims to explore, identify and test the ability of fungal isolates from potato planting areas in Brebes Regency, as well as to test rice germination on used remediation media. This research was conducted at the Laboratory of Plant Protection Mycology, Faculty of Agriculture, Jenderal Soedirman University. The research was conducted from January to October 2022. The study used a completely randomized design (CRD) with one factor, namely three mushroom isolates with cadmium concentrations (0, 25 and 50 ppm). The results showed that there were 13 isolates found and identified from the genera Trichoderma sp., Aspergillus sp., Fusarium sp., and Penicillium sp., and one isolate that had not been identified. The three best isolates based on the results of the cadmium tolerance test were isolates coded PP4 (Trichoderma sp.), WS4 (Fusarium sp.), and WS8 (Aspergillus sp.). Trichoderma sp. isolates capable of removing heavy metals in media with a Cd concentration of 25 ppm reaching 92% with a biomass capacity of 22.75 mg/g. The best biomass in the remediation ability test was isolate WS4 (Fusarium sp.) with a Cd content of 25 ppm, namely 0.69 g. The results of the rice seed germination test showed that all treatments were not significantly different in terms of wet weight, plant height, number of roots, root length, and germination. |
| Kata kunci | remediasi, jamur, kadmium |
| Pembimbing 1 | Okti Herliana, S.P., M.P. |
| Pembimbing 2 | Dr. Etik Wukir Tini, S.P., M.P. |
| Pembimbing 3 | |
| Tahun | 2022 |
| Jumlah Halaman | 16 |
| Tgl. Entri | 2022-11-25 16:34:20.50385 |