| NIM | D1A018191 |
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| Namamhs | NAURAH OLIVIA JAVANI |
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| Judul Artikel | PENAMBAHAN EKSTRAK KURMA AJWA (Phoenix dactylifera) DALAM PENGENCER SEMEN YANG DIPRESERVASI PADA SUHU 5°C TERHADAP ABNORMALITAS DAN INTEGRITAS MEMBRAN PLASMA SPERMATOZOA |
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| Abstrak (Bhs. Indonesia) | Naurah Olivia Javani. Penambahan Ekstrak Kurma Ajwa (Phoenix dactylifera) dalam Pengencer Semen yang Dipreservasi pada Suhu 5°C terhadap Abnormalitas dan Integritas Membran Plasma Spermatozoa. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh penambahan ekstrak kurma dalam pengenceran semen yang dipreservasi pada suhu 5°C terhadap abnormalitas dan integritas membran plasma spermatozoa ayam pelung. Materi yang digunakan dalam penelitian antara lain yaitu 5 ekor ayam pelung jantan yang memiliki usia 1,5 - 2 tahun, serta bahan pengencer yaitu sodium dodecyl sulfate (SDS), ekstrak kurma, kuning telur, dan ringer laktat. Rancangan penelitian yang digunakan yaitu dengan Rancangan Acak Lengkap (RAL) repeated measurement dengan lima perlakuan penambahan ekstra kurma (P0: 0 %, P1: 0,5 %, P2: 1 %, P3: 1,5 %, dan P4: 2 %) dan 4 (empat) kali ulangan. Variabel yang diamati dalam penelitian ini yaitu abnormalitas dan integritas membran plasma sel spermatozoa. Data yang diperoleh dari percobaan ditabulasikan dalam tabel tabulasi data kemudian dianalisis menggunakan analisis variansi dan dilanjutkan dengan uji lanjut Beda Nyata Jujur (BNJ). Hasil penelitian abnormalitas menunjukkan bahwa penambahan ekstrak kurma tidak berpengaruh nyata (P > 0,05) terhadap abnormalitas, sedangkan lama penyimpanan berpengaruh nyata (P < 0,05) terhadap abnormalitas. Hasil penelitian integritas membran plasma menunjukkan bahwa penambahan ekstrak kurma berpengaruh nyata (P < 0,05) terhadap integritas membran plasma, sedangkan lama penyimpanan tidak berpengaruh nyata (P > 0,05) terhadap integritas membran plasma. Kesimpulan, pemberian ekstrak kurma 0-2% menghasilkan abnormalitas yang relatif sama, sedangkan lama penyimpanan diatas 12 jam menunjukan kenaikan abnormalitas spermatozoa ayam pelung. Semakin tinggi pemberian ekstrak kurma semakin tinggi angka integritas membran plasma spermatozoa ayam pelung. |
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| Abtrak (Bhs. Inggris) | Naurah Olivia Javani. Addition of Ajwa Dates Extract (Phoenix dactylifera) in Semen Diluent Preserved at 5°C on Abnormalities and Integrity of Spermatozoa Plasma Membranes. The purpose of this study was to determine the effect of adding date extract in the dilution of semen preserved at a temperature of 5°C on the abnormality and integrity of the plasma membrane of pelung rooster spermatozoa. The materials used in the study included 5 male pelung chickens aged 1.5 - 2 years, as well as diluents, namely sodium dodecyl sulfate (SDS), date extract, egg yolk, and ringer's lactate. The research design used was a Completely Randomized Design (CRD) repeated measurement with five additional dates extra treatments (P0: 0%, P1: 0.5%, P2: 1%, P3: 1.5%, and P4: 2 %) and repeated 4 (four) times. The variables observed in this study were abnormalities and integrity of the plasma membrane of spermatozoa cells. The data obtained from the experiment were tabulated in the data tabulation table and then analyzed using analysis of variance and continued with the further test of Honest Significant Difference (BNJ). The results of the abnormality study showed that the addition of date extract had no significant effect (P > 0.05) on abnormalities, while storage time had a significant effect (P < 0.05) on abnormalities. The results of the research on the integrity of the plasma membrane showed that the addition of date extract had a sign ificant effect (P < 0.05) on the integrity of the plasma membrane, while storage time had no significant effect (P > 0.05) on the integrity of the plasma membrane. In conclusion, effect of addition of 0-2% date extract resulted in relatively the same abnormality, while storage time of more than 12 hours showed an increase in spermatozoa abnormalities in pelung roosters. The higher the date extract, the higher the plasma membrane integrity number of pelung rooster spermatozoa. |
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| Kata kunci | Ekstrak Kurma Ajwa, Pengencer Semen, Abnormalitas, Integritas, Membran Plasma Spermatozoa |
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| Pembimbing 1 | Prof. Ir. Dadang Mulyadi Saleh, M.S., M.Agr.Sc, Ph.D |
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| Pembimbing 2 | Nu’man Hidayat S.Pt., M.Si |
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| Pembimbing 3 | |
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| Tahun | 2022 |
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| Jumlah Halaman | 10 |
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| Tgl. Entri | 2022-11-13 15:52:33.955793 |
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