| NIM | A1D018090 |
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| Namamhs | YULINDA WIDI RESTANTI |
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| Judul Artikel | KARAKTERISASI MOLEKULER BERBASIS SEKUEN 16S rRNA, UJIBIOKIMIA, DAN BIOASSAY BAKTERI RESISTEN DAN DEGRADATOR PESTISIDA KLORPIRIFOS ASAL RHIZOSFER BAWANG MERAH |
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| Abstrak (Bhs. Indonesia) | Isolat bakteri yang digunakan dalam penelitian ini berasal dari lahan pertanian bawang merah Kabupaten Brebes, Kecamatan Ketanggungan dengan kode isolat BR1s dan CL5s. Identifikasi dilakukan untuk mengetahui karakter molekuler (bioinformatika dan filogenetika), biokimia (uji produksi H2S, simmons citrate, methyl red, vogues-proskauer, hidrolisis pati, hidrolisis urea, gelatinasi, oksidatif/fermentatif, dan Carboxymethyl Cellulose), Plant Growth Promoting Rhizobacteria (penambat N, pelarut P, penghasil IAA), serta pengaruh aplikasi bakteri terhadap fase perkecambahan dan karakter agronomi tanaman tomat. Hasil analisis bioinformatika menunjukkan bahwa isolat BR1s memiliki tingkat kemiripan 97,73% dengan Bacillus tropicus dan isolat CL5s memiliki tingkat kemiripan 99,93% dengan Bacillus flexus atau Priestia flexa. Kedua isolat menunjukkan respon positif pada karakterisasi biokimia dengan uji methyl-red, hidrolisis pati, gelatinasi, dan oksidatif/fermentatif. Kedua isolat menunjukkan positif terhadap karakter PGPR yaitu pelarut P dan penambat N. Hasil bioassay menunjukkan adanya pengaruh nyata aplikasi isolat bakteri BR1s terhadap variabel potensi tumbuh maksimum, daya kecambah, kecepatan tumbuh, bobot kering kecambah normal, tinggi tanaman, panjang akar, dan jumlah daun. Isolat CL5s hanya memberikan pengaruh nyata terhadap variabel jumlah daun. |
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| Abtrak (Bhs. Inggris) | The bacterial isolates used in this study came from the shallot farmland of Brebes Regency, Ketanggungan District with isolate codes BR1s and CL5s. Identification was carried out to determine molecular characters (bioinformatics and phylogenetic), biochemistry (test for H2S production, simmons citrate, methyl red, vogues-proskauer, starch hydrolysis, urea hydrolysis, gelatinization, oxidative/fermentative, and Carboxymethyl Cellulose), Plant Growth Promoting Rhizobacteria (N fixing, P solvent, producing IAA), as well as the effect of bacterial application on the germination phase and agronomic character of tomato plants. The results of the bioinformatics analysis showed that the BR1s isolate had a 97.73% similarity with Bacillus tropicus and the CL5s isolate had a 99.93% similarity with Bacillus flexus or Priestia flexa. Both isolates showed a positive response to biochemical characterization by methyl-red, starch hydrolysis, gelatinization, and oxidative/fermentative assays. Both isolates were positive for PGPR characters, namely solvent P and binding N. The results of the bioassay showed that there was a significant effect of the application of bacterial isolate BR1s on the variables of maximum growth potential, germination, growth speed, dry weight of normal sprouts, plant height, root length, and number of leaves. CL5s isolate only had a significant effect on the variable number of leaves. |
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| Kata kunci | Bioassay, Biokimia, Molekuler, Karakterisasi, Tomat |
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| Pembimbing 1 | Sapto Nugroho Hadi, S.Si., M.Biotech. |
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| Pembimbing 2 | Ida Widiyawati, S.P., M.Si |
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| Pembimbing 3 | |
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| Tahun | 2022 |
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| Jumlah Halaman | 19 |
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| Tgl. Entri | 2022-10-12 18:47:35.262094 |
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