| NIM | B1B017022 |
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| Namamhs | FAKHRI NAUFAL RAMADHAN |
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| Judul Artikel | The Production of Antimicrobial Substance by Streptomyces sp. SAE4034 at Various Temperatures and Its Activity Against Escherichia coli |
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| Abstrak (Bhs. Indonesia) | Streptomyces adalah genus dari kelas Actinomycetes yang mampu menghasilkan berbagai antibiotik dan senyawa lain yang bermanfaat secara farmasi. Sintesis dan produksi senyawa antibakteri dapat dipengaruhi oleh beberapa faktor fisiologis, termasuk suhu. Streptomyces sp. SAE4034 diisolasi dari lumpur rizosfer Rhizophora apiculata dan menunjukkan aktivitas antibakteri terhadap beberapa bakteri patogen, termasuk Escherichia coli. Penelitian ini bertujuan untuk mengetahui pengaruh suhu terhadap produksi senyawa antibakteri dan menentukan konsentrasi hambat minimum senyawa antibakteri terhadap E.coli. Hasil penelitian menunjukkan bahwa produksi ekstrak kasar oleh Streptomyces sp. SAE4034 dipengaruhi oleh suhu. Suhu inkubasi 35℃ menghasilkan persentase rendemen tertinggi 1.790 g/ml dan berat kering biomassa miselium 0,2661g. Diameter hambat zona bening tertinggi E. coli (41,5 mm) dilakukan oleh 100% Streptomyces sp. Ekstrak kasar SAE4034 sedangkan diameter hambat zona bening terendah (10,5 mm) dihasilkan oleh konsentrasi ekstrak kasar 3,125%. |
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| Abtrak (Bhs. Inggris) | Streptomyces is a genus of the Actinomycetes class which is capable of producing a variety of antibiotics and other pharmaceutically useful compounds. The synthesis and production of antibacterial compounds can be affected by several physiological factors, including temperature. Streptomyces sp. SAE4034 was isolated from Rhizophora apiculata rhizosphere mud and showed antibacterial activity against several bacterial pathogens, including Escherichia coli. This research aimed to determine the effect of temperature on the production of antibacterial compounds and to determine the minimum inhibitory concentration of the antibacterial compounds against E. coli. The result showed that the production of the crude extract by Streptomyces sp. SAE4034 was affected by temperature. The incubation temperature of 35℃ produced the highest percentage yield of 1,790 g/ml and mycelium biomass dry weight was 0,2661g. The highest clear zone inhibition diameter of E. coli (41,5 mm) was performed by 100% of Streptomyces sp. SAE4034 crude extract while the lowest clear zone inhibition diameter (10,5 mm) was produced by the concentration of crude extract 3,125%. |
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| Kata kunci | antibacterial activity, crude extract, incubation temperature, Streptomyces sp. SAE4034 |
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| Pembimbing 1 | Dr. Dini Ryandini, M.Si |
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| Pembimbing 2 | Dr. Oedjijono,M.Sc |
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| Pembimbing 3 | |
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| Tahun | 2022 |
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| Jumlah Halaman | 7 |
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| Tgl. Entri | 2022-08-12 13:58:48.722606 |
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