| NIM | I1C018058 |
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| Namamhs | FITTROTUL UYUN |
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| Judul Artikel | EKSPLORASI MEKANISME DAN TARGET AKSI SENYAWA AKTIF KAYU MANIS (Cinnamomum cassia Presl.) SEBAGAI ANTIKANKER PAYUDARA SECARA BIOINFORMATIKA |
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| Abstrak (Bhs. Indonesia) | Cinnamomum cassia telah dilaporkan memiliki aktivitas sebagai antikanker. Namun, target dan mekanisme aksi senyawa aktif C. cassia belum ditemukan pada kanker payudara. Penelitian ini bertujuan untuk mengidentifikasi target dan mekanisme senyawa aktif C. cassia terhadap kanker payudara secara bioinfomatika dan penambatan molekuler. Senyawa aktif C. cassia dari database PubMed. Gen target senyawa aktif C. cassia didapatkan dari database STITCH, STRING, dan Swiss Target Prediction yang dibandingkan dengan gen pengkode kanker payudara sehingga didapatkan Potential Theurapetic Target Genes (PTTG). PPTG dianalisis dmenggunakan STRING untuk menemukan interaksi antar protein, Cytoscape untuk mencari 10 hub genes, Web-Gestalt untuk mengetahui Gene Ontology, KEGG Pathway, dan Drug Association. Selanjutnya, penambatan molekuler dimulai dari tahap preparasi senyawa aktif C. cassia dan protein target, validasi metode dengan mengetahui nilai RSMD, dan penambatan molekuler senyawa aktif C. cassia dan protein target. Senyawa aktif C. cassia yaitu sinamaldehida, 2-hidroksi sinamaldehid, asam sinamat, sinamilalkohol, dan asam cis-2-metoksi sinamat memiliki target molekuler pada kanker payudara yaitu TP53, AKT1, TNF, IL6, JUN, MYC, EGFR, ESR1, INS, dan MAPK3. Mekanisme senyawa aktif C. cassia pada kanker payudara dengan menghambat aktivitas protein AKT1, EGFR, dan MAPK3 melalui jalur pensinyalan MAPK dan PI3K/Akt yang bertanggung jawab untuk proliferasi dan regulasi apoptosis. Penambatan molekuler dilakukan antara senyawa aktif C. cassia dan protein AKT1, EGFR, dan MAPK3. Senyawa asam cis-2-metoksi sinamat berpotensi sebagai inhibitor AKT1 dan MAPK3 karena energi ikatannya paling rendah dibandingkan senyawa aktif C. cassia lain dan kontrol lapatinib pada penambatan molekuler. |
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| Abtrak (Bhs. Inggris) | Cinnamomum cassia has been reported to have anticancer activity. However, the target and mechanism of the active compounds of C. cassia has not been found in breast cancer. This study aims to identify the target and mechanism of the active compounds C. cassia against breast cancer by bioinformatics and molecular docking. The active compound C. cassia was obtained from PubMed database. Meanwhile, the target gene for the active compound C. cassia obtained from the STITCH, STRING, and Swiss Target Prediction databases which was compared to the gene coding for breast cancer so that Potential Theurapetic Target Genes (PTTG) were obtained. PPTG was analyzed by using STRING in order to find interactions between proteins, Cytoscape to search for 10 hub genes, Web-Gestalt; besides, to determine Gene Ontology, KEGG Pathway, and Drug Association. Furthermore, the molecular docking started from the preparation stage of the active compound C. cassia and the target protein, validation of the method by knowing the RSMD value, and molecular docking of the active compound C. cassia and the target protein. The active compounds of C. cassia that are cinnamaldehyde, 2-hydroxycinnamaldehyde, cinnamic acid, cinamyl alcohol, and cis-2-methoxycinnamic acid have molecular targets in breast cancer are TP53, AKT1, TNF, IL6 , JUN, MYC, EGFR, ESR1, INS, and MAPK3. The mechanism of C. Cassia active compund in breast cancer by inhibiting the activity of AKT1, EGFR, and MAPK3 through PI3K/Akt and MAPK signaling pathways which are responsible for the proliferation and regulation of apoptosis. Molecular docking was carried out between the active compound C. cassia and the proteins AKT1, EGFR, and MAPK3. The compound cis-2-methoxycinnamic acid has potential as an inhibitor of AKT1 and MAPK3 because its binding energy is the lowest compared to other active compounds of C. cassia and the control of lapatinib in molecular docking. |
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| Kata kunci | Cinnamomum cassia, kanker payudara, bioinformatik, penambatan molekuler |
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| Pembimbing 1 | Dr. Muhamad Salman Fareza, M.Si |
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| Pembimbing 2 | apt. Nur Amalia Choironi, M.Si |
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| Pembimbing 3 | |
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| Tahun | 2022 |
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| Jumlah Halaman | 11 |
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| Tgl. Entri | 2022-06-24 11:40:43.029613 |
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