| NIM | B1A017057 |
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| Namamhs | JEHAN FAUZIAH HAFSAH |
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| Judul Artikel | PRODUKSI BAKTERIOSIN ISOLAT LG10 DAN LG15 ASAL SEDIMEN MANGROVE PANTAI LOGENDING DAN UJI PENGHAMBATANNYA TERHADAP Propionibacterium acnes |
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| Abstrak (Bhs. Indonesia) | Bakteri Asam Laktat (BAL) merupakan bakteri Gram positif yang mampu menghasilkan senyawa-senyawa metabolit seperti asam-asam organik, diasetil, hidrogen peroksida, etanol dan bakteriosin selama fermentasi gula. Bakteriosin merupakan senyawa metabolit primer yang dihasilkan oleh BAL dan bersifat bakterisidal maupun bakteriostatik. Aktivitas hambat bakteriosin berpeluang digunakan sebagai senyawa antibakteri dalam menghambat pertumbuhan bakteri penyebab jerawat Propionibacterium acnes. Isolat LG10 dan LG15 diisolasi dari sedimen mangrove Pantai Logending Kebumen, diketahui sebagai isolat BAL. Penelitian bertujuan untuk menentukan identitas isolat LG10 dan LG15, dan mengetahui aktivitas bakteriosin yang dihasilkannya dalam menghambat pertumbuhan P. acnes. Penelitian dilakukan menggunakan metode survei. Parameter utama yang diamati adalah zona hambat ekstrak kasar bakteriosin isolat LG10 dan LG15 terhadap P. acnes. Data hasil penelitian dianalisis secara deskriptif dan karakterisasi isolat bakteri dianalisis berdasarkan Bergey’s Manual of Systematic Bacteriology. Isolat BAL LG10 dan LG15 yang diisolasi dari sedimen mangrove Pantai Logending Kebumen teridentifikasi sebagai bakteri Gram positif anggota dari genus Enterococcus. Isolat LG10 dan LG15 mampu menghasilkan senyawa bakteriosin dengan aktivitas penghambatan bakteriosinnya terhadap P. acnes yaitu berturut-turut sebesar 12,5 mm dan 14 mm. Aktivitas penghambatan bakteriosin yang dihasilkan oleh kedua isolat terhadap P. acnes dikategorikan daya hambat kuat. |
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| Abtrak (Bhs. Inggris) | Lactic Acid Bacteria (LAB) are Gram positive bacteria capable of producing metabolites such as organic acids, diacetyl, hydrogen peroxide, ethanol and bacteriocins during sugar fermentation. Bacteriocins are primary metabolites produced by LAB and are bactericidal and bacteriostatic. The inhibitory activity of bacteriocin has the opportunity to be used as an antibacterial compound in inhibiting the growth of the acne-causing bacteria, Propionibacterium acnes. LG10 and LG15 isolates were isolated from the mangrove sediments of Logending Beach, Kebumen, known as LAB isolates. The aim of this study was to determine the identity of the isolates LG10 and LG15 and to determine the activity of the bacteriocins they produce in inhibiting the growth of P. acnes. The research was conducted using a survey method. The main parameter observed was the diameter of the inhibition zone of the crude bacteriocin against P. acnes. The research data were analyzed descriptively and the characterization of bacterial isolates was analyzed based on Bergey's Manual of Systematic Bacteriology. LAB isolates LG10 and LG15 isolated from mangrove sediments at Logending Beach, Kebumen were identified as Gram-positive bacteria belonging to the genus Enterococcus. LG10 and LG15 isolates were able to produce bacteriocin compounds with bacteriocin inhibitory activity against P. acnes which were 12.5 mm and 14 mm, respectively. The bacteriocin inhibitory activity produced by the two isolates against P. acnes was categorized as strong inhibitory. |
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| Kata kunci | Bakteri Asam Laktat, bakteriosin, jerawat, Propionibacterium acnes |
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| Pembimbing 1 | Dra. Dyah Fitri Kusharyati, M.P. |
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| Pembimbing 2 | Dr. Dini Ryandini, M.Si. |
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| Pembimbing 3 | |
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| Tahun | 2022 |
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| Jumlah Halaman | 15 |
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| Tgl. Entri | 2022-05-19 16:37:20.529159 |
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