| NIM | D1A018118 |
|---|
| Namamhs | Imam Wahyudi |
|---|
| Judul Artikel | ISOLASI DAN EKSTRAKSI DAUN KALIANDRA (Calliandra calothyrsus) SEBAGAI KONSENTRAT PROTEIN DAUN DITINJAU DARI TITIK ISOELEKTRIK, ASAM AMINO DAN ZAT ANTINUTRISI |
|---|
| Abstrak (Bhs. Indonesia) | Penelitian “Isolasi dan Ekstraksi Daun Kaliandra (Calliandra calothyrsus) sebagai Konsentrat Protein Daun Ditinjau dari Titik Isoelektrik, Asam Amino dan Zat Antinutrisi”. Penelitian ini bertujuan untuk mengetahui titik isoelektrik, kandungan asam amino total dan kandungan zat antinutrisi tanin pada konsentrat protein daun kaliandra hasil ekstraksi dan isolasi protein. Materi penelitian yang digunakan adalah daun kaliandra (Calliandra calothyrsus). Penentuan titik isoelektrik menggunakan metode modifikasi Yatno (2009), kandungan asam amino total menggunakan metode high-performance liquid chromatography (HPLC), analisis protein kasar menggunakan metode Kjedhal menurut AOAC (2005), dan kandungan zat antinutrisi tanin menggunakan metode butanol-HCl. Hasil penelitian menunjukkan titik isoelektrik konsentrat protein daun kaliandra tercapai pada ekstraksi pertama dengan NaOH 0,05 Ndan buffer fosfat pH 8 pada ekstraksi kedua menghasilkan 7,2 mg/ml endapan protein dengan kadar protein kasar 48,94%. Komposisi asam amino konsentrat protein daun kaliandra lebih tinggi dibandingkan komposisi asam amino bungkil kedelai kecuali glutamin. Kadar tanin yang dihasilkan pada penelitian ini sebesar 0,01% sehingga konsentrat protein daun kaliandra aman digunakan sebagai bahan pakan sumber protein pada ransum unggas. |
|---|
| Abtrak (Bhs. Inggris) | Research "Isolation and Extraction of Calliandra (Calliandra calothyrsus) Leaf as Leaf Protein Concentrate Judging from Isoelectric Point, Amino Acids and Antinutrients". This study aims to determine the isoelectric point, total amino acid content and the content of antinutrient tannins in the protein concentrate of calliandra leaves extracted and isolated from the protein. The research material used was calliandra (Calliandra calothyrsus) leaves. Determination of the isoelectric point using the modified Yatno method (2009), the total amino acid content using the high-performance liquid chromatography (HPLC) method, crude protein analysis using the Kjedhal according to AOAC (2005), and the content of antinutrient tannins using the butanol-HCl method. The results showed that the isoelectric point of the calliandra leaf protein concentrate was reached in the first extraction with 0.05 N NaOH and pH 8 phosphate buffer in the second extraction resulting in 7.2 mg/ml protein precipitate with a crude protein content of 48.94%. The amino acid composition of calliandra leaf protein concentrate was higher than that of soybean meal except for glutamine. The tannin content produced in this study was 0.01% so that the calliandra leaf protein concentrate was safe to use as a protein source in poultry rations. |
|---|
| Kata kunci | daun kaliandra, protein, titik isoelektrik, asam amino, tanin |
|---|
| Pembimbing 1 | Dr. Ir. Efka Aris Rimbawanto, MP |
|---|
| Pembimbing 2 | Dr. Ir. Bambang Hartoyo, M.Si |
|---|
| Pembimbing 3 | |
|---|
| Tahun | 2022 |
|---|
| Jumlah Halaman | 9 |
|---|
| Tgl. Entri | 2022-05-17 09:22:30.731593 |
|---|