| NIM | A1D017110 |
|---|
| Namamhs | NUR ANNISA PRIYATI |
|---|
| Judul Artikel | UJI KEMAMPUAN BAKTERI RHIZOSFER DALAM MENGENDALIKAN PENYAKIT HAWAR PELEPAH JAGUNG |
|---|
| Abstrak (Bhs. Indonesia) | Jagung merupakan komoditas penting yang mempunyai peranan strategis dalam pembangunan pertanian sebagai bahan pangan, bahan pakan, bahan bakar, dan bahan baku industri. Kendala pada proses budidaya jagung diantaranya yaitu penyakit hawar pelepah akibat jamur patogen Rhizoctonia solani. Pengendalian alternatif ramah lingkungan dengan memanfaatkan bakteri Bacillus spp. dan Pseudomonas fluorescens dilakukan untuk menekan perkembangan penyakit hawar pelepah. Penelitian ini bertujuan untuk menguji kemampuan bakteri rhizosfer Pseudomonas fluorescens dan Bacillus sp. terhadap pertumbuhan jamur patogen Rhizoctonia solani secara in vitro, mengetahui efektivitas bakteri Pseudomonas fluorescens dan Bacillus sp. dalam mengendalikan penyakit hawar pelepah pada tanaman jagung secara in vivo, dan mengetahui pengaruh aplikasi bakteri Pseudomonas fluorescens dan Bacillus sp. asal rhizosfer terhadap pertumbuhan tanaman jagung. Penelitian ini menggunakan Rancangan Acak Kelompok dengan perlakuan bakteri rhizosfer Bacillus sp. R3, Bacillus sp. R5, Pseudomonas fluorescens R1. Variabel yang diamati yaitu pengujian in vitro meliputi penghambatan pertumbuhan R. solani dan bobot kering miselium; pada pengujian in vivo meliputi masa inkubasi penyakit, intensitas penyakit, kejadian penyakit, AUDPC, serta komponen pertumbuhan tanaman. Hasil penelitian menunjukkan bahwa bakteri Bacillus sp. R3 mampu menghambat pertumbuhan jamur R. solani dengan tingkat penghambatan tertinggi sebesar 69,91% dan nilai bobot kering miselium terkecil sebesar 0,20 g. Hasil pengujian secara in vivo perlakuan bakteri Bacillus sp. R3 memberikan pengaruh terbaik dalam mengendalikan penyakit hawar pelepah dengan menunda masa inkubasi selama 19 hari, penekanan intensitas penyakit sebesar 51,06% dan nilai AUDPC sebesar 53,40%. Bakteri Pseudomonas fluorescens R1 memiliki pengaruh terbaik terhadap pertumbuhan tanaman jagung dalam meningkatkan tinggi tanaman sebesar 142,6 cm; jumlah daun 18,95; bobot segar tanaman 89,78 g, dan bobot kering akar 2,01 g. |
|---|
| Abtrak (Bhs. Inggris) | Maize is an important commodity with strategic role in agricultural development as food, feed, fuel and industrial raw materials. There is an obstacle during the maize cultivation process, out of which sheath blight disease is caused by the pathogenic fungus Rhizoctonia solani. Using rhizosphere bacteria such as Bacillus sp. and Pseudomonas fluorescens would be an alternative method to control the disease. This research aims to determine the ability of rhizosphere bacteria Pseudomonas fluorescens and Bacillus sp. in inhibiting the growth of the pathogenic fungus R. solani in vitro, to identify the effectiveness of the rhizosphere bacteria Pseudomonas fluorescens and Bacillus sp. in controlling sheath blight on maize in vivo, and to observe the effect of application of rhizosphere bacteria Pseudomonas fluorescens and Bacillus sp. on maize growth. The research design used was a Randomized Block Design with 1 factor, the treatment of rhizosphere bacteria. The variables observed were in vitro testing including inhibition of the growth of the fungus R. solani and dry weight of mycelium; In vivo testing includes disease incubation period, disease intensity, disease incidence, AUDPC, and plant growth components. The result showed that the treatment of Bacillus sp. R3 gave the best effect in inhibiting R. solani with the inhibition level of 69,91% and dry weight of mycelium with the value 0,20 g. Bacillus sp. R3 gave the best effect in vivo testing; delayed the incubation period for 19 days, reduced the intensity of the disease by 51.06% and the AUDPC value by 53.40%. Application of rhizosphere bacteria Pseudomonas fluorescens R1 gave the best effect on the growth of maize plants in increasing plant height by 142.6 cm; number of leaves 18.95; plant fresh weight 89.78 g, and root dry weight 2.01 g. |
|---|
| Kata kunci | bakteri rhizosfer, Bacillus sp., P. fluorescens, R. solani, jagung |
|---|
| Pembimbing 1 | Endang Mugiastuti, S.P., M.P. |
|---|
| Pembimbing 2 | Ir. Joko Maryanto, M.Si. |
|---|
| Pembimbing 3 | |
|---|
| Tahun | 2022 |
|---|
| Jumlah Halaman | 15 |
|---|
| Tgl. Entri | 2022-02-17 16:10:05.202107 |
|---|