Artikel Ilmiah : G1A018046 a.n. ASHLABIELLAH NUR SAFAH LUBIS
| NIM | G1A018046 |
|---|---|
| Namamhs | ASHLABIELLAH NUR SAFAH LUBIS |
| Judul Artikel | PERBEDAAN KADAR INTERLEUKIN 6 (IL-6) PASCA INDUKSI BERBAGAI MODEL STRES SLEEP DEPRIVATION TIKUS PUTIH (Rattus norvegicus) JANTAN |
| Abstrak (Bhs. Indonesia) | Latar Belakang: Sitokin proinflamasi Interleukin 6 merupakan salah satu biomarker spesifik yang dilepaskan selama sleep deprivation. Studi menunjukkan bahwa tingginya kadar IL-6 plasma berhubungan dengan morbiditas dan mortalitas. Sleep recovery dinilai dapat memperbaiki dampak dari sleep deprivation dengan menyeimbangkan molekul sitokin pro-inflamasi dengan anti-inflamasi pada sirkulasi. Tujuan: Mengetahui perbedaan kadar Interleukin 6 (IL-6) pada tikus putih (Rattus norvegicus) jantan pasca induksi berbagai model stres sleep deprivation. Metode: Penelitian eksperimental dengan post-test only with control group design yang dilakukan pada 25 ekor hewan coba tikus putih (Rattus norvegicus) jantan galur Wistar. Hewan coba dibagi secara acak menjadi 5 kelompok percobaan yaitu kelompok kontrol, PSD (20 jam SD/hari selama 5 hari), TSD (24 jam SD/hari selama 5 hari), PSD+SR (20 jam SD/hari selama 5 hari dilanjutkan SR selama 5 hari), dan TSD+SR (24 jam SD/hari selama 5 hari dilanjutkan SR selama 5 hari). Model sleep deprivation menggunakan modified multiple platform method (MMPM). Kadar IL-6 diukur menggunakan Bead-based Multiplex Assays dengan teknologi Luminex. Hasil: Uji One-way ANOVA menunjukkan tidak ada perbedaan yang signifikan pada kadar IL-6 antar kelompok hewan coba (p=0,658). Rerata kadar IL-6 tertinggi pada kelompok TSD (1578 pg/ml) diikuti kelompok PSD (1281 pg/ml), PSD+SR (1192 pg/ml), kontrol (974 pg/ml), dan TSD+SR (952 pg/ml). Kesimpulan: Tidak terdapat perbedaan kadar interleukin 6 (IL-6) yang signifikan pada tikus putih (Rattus norvegicus) jantan pasca induksi berbagai model stres sleep deprivation. |
| Abtrak (Bhs. Inggris) | Introduction: Pro-inflammatory cytokine IL-6 is a specific biomarkers that are released during sleep deprivation. Studies show that IL-6 levels is associated with morbidity and mortality. Sleep recovery is considered to be able to restore the effects of sleep deprivation by balancing pro-inflammatory and anti-inflammatory cytokines molecules in the circulation. Objective: To determine the differences in the levels of Interleukin 6 (IL-6) in male albino rats (Rattus norvegicus) after induction various stress models of sleep deprivation. Methods: Experimental research with post-test only and control group design on 25 male albino rats were distributed into 5 groups consist of control group, PSD (20 hours of SD/day for 5 days), TSD (24 hours of SD/days for 5 days), PSD+SR (20 hours SD/days for 5 days then SR for 5 days), and TSD+SR (24 hours SD/days for 5 days then SR for 5 days). Sleep deprivation model used a modified multiple platform method (MMPM). Interleukin 6 levels were measured using Bead-based Multiplex Assays with Luminex technology. Results: One-way ANOVA test shows that there is no significant difference in IL-6 levels (p=0.658). The highest mean IL-6 level is in the TSD group (1578 pg/ml) followed by the PSD group (1281 pg/ml), PSD+SR (1192 pg/ml), control (974 pg/ml) and TSD+SR (952pg/ml). Conclusions: There is no significant difference in IL-6 levels in male albino rats (Rattus norvegicus) after induction of various sleep deprivation stress models |
| Kata kunci | interleukin 6 (IL-6), sitokin, sleep deprivation, sleep recovery |
| Pembimbing 1 | Dr. dr. Fitranto Arjadi, M. Kes. |
| Pembimbing 2 | Dr. dr. Joko Setyono, M. Sc. |
| Pembimbing 3 | |
| Tahun | 2022 |
| Jumlah Halaman | 12 |
| Tgl. Entri | 2022-01-18 11:25:34.57596 |