| NIM | B1A017051 |
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| Namamhs | BRAMASSETYO AJI |
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| Judul Artikel | Efek Perbedaan Temperatur Terhadap Aktivitas Pepsin dan Tripsin-Like Ikan Lele (Clarias gariepinus) |
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| Abstrak (Bhs. Indonesia) | Ikan lele (Clarias gariepinus) merupakan ikan air tawar yang sering dibudidayakan oleh masyarakat. Ikan lele adalah ikan omnivora yang cenderung karnivora sehingga aktivitas proteasenya tinggi. Protease berdasarkan pH kerjanya terdiri atas protease asam contohnya pepsin di lambung dan protease basa contohnya tripsin-like di intestin. Studi tentang aktivitas pepsin dan tripsin-like dengan perbedaan temperatur pada ikan lele belum banyak dilakukan. Penelitian ini bertujuan untuk mengetahui pengaruh temperatur yang berbeda dan mengetahui temperatur optimum dari aktivitas pepsin dan tripsin–like ikan lele (Clarias gariepinus). Penelitian ini menggunakan metode eksperimental dengan rancangan percobaan berupa rancangan acak lengkap (RAL) pada temperatur 20°C, 30°C, 40°C, 50°C, 60°C dan 70°C dalam lima kali ulangan. Penelitian ini menggunakan 60 ekor ikan lele dengan berat 21,373±2,887 g dan panjang 15,244±1,262 cm dari pengepul ikan lele di Kabupaten Banyumas, Jawa Tengah. Variabel penelitian ini adalah temperatur, aktivitas pepsin dan tripsin-like. Parameter pada penelitian ini adalah kadar tirosin diukur dengan metode spektrofotometer pada λ 720nm. Analisis data dilakukan dengan one way ANOVA, dilanjutkan dengan uji lanjut Beda Nyata Jujur (BNJ) dengan tingkat kesalahan 5%. Hasil penelitian ini yaitu perbedaan temperatur inkubasi 20°C, 30°C, 40°C, 50°C, 60°C dan 70°C berpengaruh nyata terhadap aktivitas pepsin dan tripsin-like (p<0,05). Aktivitas pepsin tinggi berkisar pada temperatur 20-40°C dan aktivitas tripsin-like tinggi berkisar pada temperatur 40-60°C. Kesimpulan penelitian ini perbedaan temperatur berpengaruh nyata terhadap peningkatan dan penurunan aktivitas pepsin dan tripsin-like. Aktivitas pepsin tinggi berkisar pada temperatur 20-40°C dan tripsin-like tinggi berkisar pada temperatur 40-60°C. |
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| Abtrak (Bhs. Inggris) | Catfish (Clarias gariepinus) is a freshwater fish that is commonly cultivated by the local community. Catfish are omnivorous fish that are more likely to be carnivorous, therefore catfish proteases have high activity. Proteases based on their optimum pH consist of acidic proteases such as pepsin in the stomach and alkaline proteases such as trypsin-like in the intestine. Previous studies on pepsin and trypsin-like activity to temperature differences in catfish has not been widely carried out. This research aims are (1) to determine the influence of different temperatures and (2) to determine the optimum temperature on the activity of pepsin and trypsin-like catfish (Clarias gariepinus). This research uses experimental methods to the experimental design in the form of completely randomized design (CRD) at temperatures of 20°C, 30°C, 40°C, 50°C, 60°C and 70°C in five repetitions. This study used 60 catfish with weighing 21,373±2,887 g and length of 15,244±1,262 cm from fishmonger in Banyumas Regency, Central Java. The variables of this study were temperature, pepsin activity and trypsin-like activity. The parameter in this study is the tyrosine level which will be measured by the spectrophotometer method at λ 720nm. Data analysis was carried out using one way ANOVA, followed by Tukey’s HSD Test with an error rate of 5%. The results of this study were that the difference in temperature of 20°C, 30°C, 40°C, 50°C, 60°C and 70°C significantly affected pepsin and trypsin-like activity (p<0.05). High pepsin activity ranges from 20-40°C and high trypsin-like activity ranges from 40-60°C. The conclusion of this study were that the temperature difference significantly affected on increasing and decreasing of pepsin and trypsin-like activity. The high activity of pepsin was at 20-40°C and high activity of trypsin-like was at a temperature of 40-60°C. |
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| Kata kunci | Clarias gariepinus; pepsin; temperatur; tripsin-like |
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| Pembimbing 1 | Dr. Untung Susilo, M. S. |
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| Pembimbing 2 | Dr. Farida Nur Rachmawati, M. Si. |
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| Pembimbing 3 | |
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| Tahun | 2021 |
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| Jumlah Halaman | 11 |
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| Tgl. Entri | 2021-08-16 13:54:25.963032 |
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