| NIM | B1B017025 |
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| Namamhs | SYAMSUL MA'ARIF |
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| Judul Artikel | Determination of Streptomyces sp. SAE4034 Bioactive Substances as Antibacterial on Pseudomonas aeruginosa and Antiangiogenic Properties |
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| Abstrak (Bhs. Indonesia) | Streptomyces sp. SAE4034, an actinomycetes, is potentially produce bioactive substances which can be used for treating such infectious disease and cancer. The development of bioactive screening on actinomycetes became a challenge. There are still many species whose existence and function in nature have not been studied. It is possible to discover and study the further potency related to new bioactive agents such as antibacterial and antiangiogenic properties. This research was conducted to know the type of antibacterial and antiangiogenic compound produced by Streptomyces sp. SAE4034, to observe the inhibition mechanism of Streptomyces sp. SAE4034 crude extract against P. aeruginosa, and to observe the antiangiogenic activity Streptomyces sp. SAE4034 crude extract on chicken embryo. The result of showed that Streptomyces sp. SAE4034 crude extract separated into different spots (Rf 0.02, 0.09, 0.25, 0.49, 0.63, 0.79, and 0.95). Polyphenol detected on Rf 0.02,0.49, and 0.95; flavonoid detected on Rf 0.09 and 0.95; Terpenoid detected smear over Rf 0.02-0.49 and 0.95; while polyketide detected on Rf 0.02-0.49 and 0.95. The crude extract potentially inhibited P. aeruginosa by losing the nucleic acid and protein content of the cells. Potential antiangiogenic substances were proven by the crude extract activity on embryonated chicken eggs with results 50 µg/ml (mean= 23.10%) and 100 µg/ml (mean= 11.77%) respectively inhibit the growth and sprouting of blood vessels |
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| Abtrak (Bhs. Inggris) | Streptomyces sp. SAE4034, an actinomycetes, is potentially produce bioactive substances which can be used for treating such infectious disease and cancer. The development of bioactive screening on actinomycetes became a challenge. There are still many species whose existence and function in nature have not been studied. It is possible to discover and study the further potency related to new bioactive agents such as antibacterial and antiangiogenic properties. This research was conducted to know the type of antibacterial and antiangiogenic compound produced by Streptomyces sp. SAE4034, to observe the inhibition mechanism of Streptomyces sp. SAE4034 crude extract against P. aeruginosa, and to observe the antiangiogenic activity Streptomyces sp. SAE4034 crude extract on chicken embryo. The result of showed that Streptomyces sp. SAE4034 crude extract separated into different spots (Rf 0.02, 0.09, 0.25, 0.49, 0.63, 0.79, and 0.95). Polyphenol detected on Rf 0.02,0.49, and 0.95; flavonoid detected on Rf 0.09 and 0.95; Terpenoid detected smear over Rf 0.02-0.49 and 0.95; while polyketide detected on Rf 0.02-0.49 and 0.95. The crude extract potentially inhibited P. aeruginosa by losing the nucleic acid and protein content of the cells. Potential antiangiogenic substances were proven by the crude extract activity on embryonated chicken eggs with results 50 µg/ml (mean= 23.10%) and 100 µg/ml (mean= 11.77%) respectively inhibit the growth and sprouting of blood vessels |
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| Kata kunci | Antiangiogenic, Antibacterial, Bioactive Substances, Inhibition Mechanism, Streptomyces sp. SAE4034 crude extract |
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| Pembimbing 1 | Dr. Dini Ryandini, M.Si |
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| Pembimbing 2 | Dra. Dyah Fitri Kusharyati, M.P |
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| Pembimbing 3 | |
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| Tahun | 2021 |
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| Jumlah Halaman | 9 |
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| Tgl. Entri | 2021-08-14 06:08:00.396934 |
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