Artikel Ilmiah : I1C015095 a.n. MUHAMMAD HEYKAL PUTRA
| NIM | I1C015095 |
|---|---|
| Namamhs | MUHAMMAD HEYKAL PUTRA |
| Judul Artikel | Aktivitas Ekstrak etanol Daun Lidah Buaya (Aloe vera Burm.F) Terhadap Menghambat Pertumbuhan Trychophyton rubrum |
| Abstrak (Bhs. Indonesia) | Latar Belakang: Dermatofitosis adalah penyakit akibat kolonisasi jamur dermatofit dengan agen penyebab terbanyak yaitu jamur Trychophyton rubrum. Lidah buaya mempunyai senyawa yang dapat digunakan sebagai antijamur Tujuan penelitian ini adalah menentukan efek ekstrak etanol daun lidah buaya sebagai antifungi terhadap Trychophyton rubrum, menentukan konsentrasi ekstrak etanol lidah buaya yang efektif sebagai antifungi dan menentukan diameter zona hambatan ekstrak etanol lidah buaya terhadap Trychophyton rubrum. Metodologi: Penelitian ini merupakan penelitian eksperimental, biakan Trichophyton rubrum murni diperoleh dari Laboratorium Sentral Universitas Padjajaran. Pengambilan sampel diinokulasi dengan sampling acak dengan kriteria umur jamur 2-3 hari. Koloni Trichophyton rubrum pada potatoe Dextrose Agar diambil kemudian dilarutkan dengan 100 ml aquadest sampai mencapai kekeruhan, kemudian dioleskan merata pada cawan petri yang berisi agar Potatoe. Sampel dibagi menjadi 3 kelompok, yaitu kontrol negatif berupa DMSO dan kontrol positif berupa ketokonazole, ekstrak dengan konsentrasi 2.5 %, 5% dan 10. Masing-masing kelompok terdiri dari 5 sumuran. Seluruh cawan petri diinkubasi selama 5-7 hari dengan suhu kamar. Data berupa diameter zona hambat dianalisis menggunakan uji Difusi. Hasil Penelitian: Hasil Ekstrak lidah buaya zona hambat Trychophyton rubrum menunjukan bahwa untuk konsentrasi 2.5 % tidak terdapat hambatan terhadap Trychophyton rubrum untuk 5% tidak terdapat hambatan terhadap Trychophyton rubrum dan untuk 10% tidak terdapat hambatan terhadap Trychophyton rubrum. Kemudian dilakukan pengulangan sebanyak 2 kali, dan hasilnya tidak terdapat hambatan terhadap Trychophyton rubrum. Kesimpulan: Berdasarkan dari penelitian serta uraian pembahasan di atas dapat disimpulkan bahwa ekstrak lidah buaya (Aloe vera Burm.F) pada konsentrasi 2.5%, 5%, dan 10% tidak memiliki pengaruh terhadap Trychophyton rubrum. Kata kunci: Lidah buaya, Antijamur, Trychophyton rubrum |
| Abtrak (Bhs. Inggris) | Background: Dermatophytosis is a disease caused by dermatophyte fungal colonization with the most common causative agent being the fungals Trychophyton rubrum. Aloe vera has compounds that can be used as antifungals. The purpose of this study was to determine the effect of ethanol extract of aloe vera leaves as antifungal against Trychophyton rubrum, determine the concentration of ethanolic extract of aloe vera which is effective as antifungal and determine the diameter of the inhibition zone of aloe vera ethanol extract against Trychophyton rubrum. Methodology: This study is an experimental study, Trichophyton rubrum were obtained from the Central Laboratory of Padjajaran University. Sampling was inoculated by random sampling with the criteria of mushroom age 2-3 days. Trichophyton rubrum colonies on potatoe Dextrose Agar were taken and then dissolved with 100 ml of aquadest until it reached turbidity, then spread evenly on a petri dish containing Potatoe agar. The samples were divided into 3 groups, namely negative control in the form of DMSO and positive control in the form of ketoconazole, extract with a concentration of 2.5%, 5% and 10. Each group consisted of 5 wells. All petri dishes were incubated for 5-7 days at room temperature. Data in the form of the diameter of the inhibition zone were analyzed using the Diffusion test. Results: The results of Aloe vera extract in the inhibitory zone of Trychophyton rubrum showed that for a concentration of 2.5% there was no inhibition against Trychophyton rubrum for 5% there was no inhibition against Trychophyton rubrum and for 10% there was no inhibition against Trychophyton rubrum. Then it was repeated 2 times, and the result was that there were no obstacles to Trychophyton rubrum. Conclusion: Based on the research and the description of the discussion above, it can be concluded that the extract of aloe vera (Aloe vera Burm.F) at concentrations of 2.5%, 5%, and 10% had no effect on Trychophyton rubrum. Keywords: Aloe vera, Antifungal, Trychophyton rubrum |
| Kata kunci | Lidah buaya, Antijamur, Trychophyton rubrum |
| Pembimbing 1 | Drs. Sunarto, MS.MP |
| Pembimbing 2 | Apt. Nur Amalia C., M.Si. |
| Pembimbing 3 | |
| Tahun | 2021 |
| Jumlah Halaman | 17 |
| Tgl. Entri | 2021-08-04 12:35:49.347218 |