| NIM | D1A016052 |
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| Namamhs | DIPTA RILANDANA |
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| Judul Artikel | PENGARUH MACAM PENGENCER DAN LAMA PENYIMPANAN PADA SUHU 5oC TERHADAP MOTILITAS, VIABILITAS DAN ABNORMALITAS SPERMATOZOA AYAM KAMPUNG |
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| Abstrak (Bhs. Indonesia) | Pengaruh Macam Pengencer dan Lama Penyimpanan Pada Suhu 5oC Terhadap Motilitas, Viabilitas dan Abnormalitas Spermatozoa Ayam Kampung. Penelitian dilaksanakan di Laboratorium Fisiologi dan Reproduksi Ternak Fakultas Peternakan Universitas Jenderal Soedirman, tanggal 1 sampai dengan 7 Oktober 2020. Tujuan penelitian untuk mengetahui interaksi macam pengencer (ringer laktat, susu skim, dan andromed) dan lama waktu penyimpanan pada suhu 5°C terhadap motilitas, viabilitas, dan abnormalitas spermatozoa ayam kampung. Materi penelitian yang digunakan yaitu ayam kampung jantan umur 1-1,5 tahun sebanyak 7 ekor sebagai penghasil spermatozoa, pakan ayam, kandang baterai beserta perlengkapannya, gelas penampung spermatozoa, seperangkat alat untuk uji kualitas spermatozoa, dan spuit 1 ml. Materi lainnya adalah spermatozoa segar dari 7 ekor ayam kampung, susu skim, ringer laktat, andromed, aquades, mikroskop, pipet tetes, object glass, cover glass, box semen, bilik thoma, pipet hematokrit, larutan NaCl 3%, larutan eosin negrosin, kulkas, dan microtube. Rancangan penelitian yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan pola faktorial 2 faktor: faktor P yaitu macam pengencer dan faktor W lama simpan pada suhu 5°C melalui 3 perlakuan. Terdapat 3 ulangan dalam setiap perlakuan sehingga terdapat 27 unit percobaan dengan masing-masing perlakuan terdiri dari 3 waktu yang berbeda. Hasil penelitian menunjukkan bahwa interaksi antar perlakuan macam pengencer dan lama penyimpanan memberikan pengaruh yang nyata (P<0,05) terhadap viabilitas spermatozoa ayam kampung, sedangkan interaksi antar perlakuan macam pengencer dan lama penyimpanan pada motilitas dan abnormalitas spermatozoa ayam kampung berpengaruh tidak nyata (P>0,05). Hasil ANOVA pengaruh macam pengencer berpengaruh sangat nyata (P<0,01) terhadap motilitas sedangkan pada abnormalitas berpengaruh tidak nyata (P>0,05). Lama penyimpanan berpengaruh nyata (P<0,05) terhadap abnormalitas dan berpengaruh tidak nyata (P>0,05) terhadap motilitas. Hasil menunjukkan motilitas terbaik spermatozoa ayam kampung dihasilkan oleh pengencer ringer laktat dengan penyimpanan selama 10 menit (92%). viabilitas terbaik spermatozoa ayam kampung dihasilkan oleh pengencer andromed dengan penyimpanan selama 370 menit (96,5%). Selanjutnya, abnormalitas terendah spermatozoa ayam kampung dihasilkan oleh pengencer susu skim dengan penyimpanan selama 190 menit (8,5%). |
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| Abtrak (Bhs. Inggris) | The Effect of Kinds of Diluents and Storage Time at 5°C on Motility, Viability and Abnormality of Kampung Rooster Spermatozoa. The research was conducted at the Laboratory of Animal Physiology and Reproduction, Faculty of Animal Husbandry, Jenderal Soedirman University, from 1 to 7 October 2020. The aim of the study was to determine the interaction between types of diluents (Ringer's lactate, skimmed milk, and andromed) and storage time at 5°C on motility, viability, and abnormalities of kampung rooster spermatozoa. The research materials used were 7 kampung rooster aged 1-1.5 years as a spermatozoa producer, chicken feed, battery cage and its equipment, spermatozoa storage glass, a set of tools to test the quality of spermatozoa, and 1 ml syringe. Other materials are fresh spermatozoa from 7 free-range kampung rooster, skim milk, lactated Ringer, andromed, distilled water, microscopes, dropper pipettes, object glass, cover glass, cement box, thoma chamber, hematocrit pipette, 3% NaCl solution, eosin negrosin solution, refrigerator, and microtube. The research design used was a completely randomized design (CRD) with a factorial pattern of 2 factors: the P factor, namely the kind of diluent and the W factor, the time of storage at 5°C through 3 treatments. There were 3 replications in each treatment so that there were 27 experimental units with each treatment consisting of 3 different times. The results showed that the interaction between different kinds of diluent treatments and storage time had a significant effect (P<0.05) on the viability of kampung rooster spermatozoa, while the interaction between treatments of types of diluents and storage time on motility and abnormalities of kampung rooster spermatozoa had no significant effect (P>0.05). ANOVA results had a very significant effect on the type of diluent (P<0.01) on motility, while the abnormal effect was not significant (P>0.05). Storage time had a significant (P<0.05) effect on abnormalities and had no significant (P>0.05) effect on motility. The results showed that the best motility of free-range kampung rooster spermatozoa was produced by Ringer's lactate diluent with storage for 10 minutes (92%). The best viability of kampung rooster spermatozoa was produced by andromed diluent with storage for 370 minutes (96.5%). Furthermore, the lowest abnormality of kampung rooster spermatozoa was produced by skim milk diluent with storage for 190 minutes (8.5%). |
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| Kata kunci | Ayam kampung, spermatozoa, motilitas, viabilitas, abnormalitas. |
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| Pembimbing 1 | Prof. Ir. Dadang Mulyadi Saleh, M.S., M.Agr.Sc., Ph.D |
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| Pembimbing 2 | Aras Prasetiyo Nugroho, S.Pt., M.Si |
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| Pembimbing 3 | |
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| Tahun | 2021 |
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| Jumlah Halaman | 8 |
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| Tgl. Entri | 2021-04-15 14:39:44.658101 |
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