Artikel Ilmiah : G1A017003 a.n. MOHAMMAD IQBAL RAMADHAN
| NIM | G1A017003 |
|---|---|
| Namamhs | MOHAMMAD IQBAL RAMADHAN |
| Judul Artikel | PENGARUH EKSTRAK ETANOL DAUN KEMBANG BULAN (Tithonia diversifolia) TERHADAP KADAR UREUM-KREATININ PADA TIKUS SPRAGUE DAWLEY YANG DIINDUKSI NICOTINAMIDE-STREPTOZOTOCIN |
| Abstrak (Bhs. Indonesia) | Latar Belakang: Penyakit ginjal diabetes merupakan penyebab utama gagal ginjal yang ditandai dengan peningkatan kadar ureum dan kreatinin. Ekstrak daun Tihtonia diversifolia merupakan ramuan herbal yang digunakan pada masyarakat untuk penyakit diabetes melitus dan secara ilmiah mampu menurunkan kadar glukosa darah. Tujuan: Tujuan penelitian ini adalah untuk mengetahui efek perlindungan ekstrak daun Tithonia diversifolia dalam mencegah peningkatan kadar ureum dan kreatinin pada tikus diabetes. Metode penelitian: Penelitian ini merupakan penelitian eksperimental dengan post test only with control group design. Dua puluh ekor tikus putih dibagi dalam 5 kelompok. Kelompok 1: kontrol sehat (SO, n=4), kelompok 2: kontrol sakit (SN, n=4), kelompok 3 (n=4), kelompok 4 (n=4), dan kelompok 5 (n=4) adalah kelompok sakit dan diberikan ekstrak etanol daun kembang bulan 25 mg/kgBB, 50 mg/kgBB, dan 100 mg/kgBB selama 28 hari. Hasil: Kadar ureum pada kelompok 1,2,3,4 dan 5 masing-masing adalah 28,8 ± 1,3; 75,0 ± 14,3; 59,2 ± 17,5; 51,0 ± 15,7; dan 35,0 ± 3,6 mg/dL. Selanjutnya kadar kreatinin pada kelompok 1,2,3,4 dan 5 masing-masing 0,9675 ± 0,033; 1,1625 ± 0,057; 1,1325 ± 0,144; 1,025 ± 0,075; dan 0,9875 ± 0,106 mg/dL. Uji statistik menunjukkan bahwa terdapat perbedaan kadar ureum-kreatinin kelompok 5 dengan kelompok 2 (p=0,000;p=0,016), sedangkan antara kelompok 1 tidak ada perbedaan (p=0,487;p=0,762). Kesimpulan: Ekstrak daun Tithonia diversifolia dengan dosis 100 mg/KgBB dapat mencegah peningkatan kadar ureum-kreatinin pada tikus diabetes. |
| Abtrak (Bhs. Inggris) | Background: Diabetic kidney disease is a major cause of kidney failure characterized by increased levels of urea and creatinine. Tithonia diversifolia leaf extract is an herb that is used for diabetes mellitus in the community and is scientifically able to reduce blood glucose levels. Goals: The purpose of this study was to determine the protective effect of Tithonia diversifolia leaf extract to prevent the increase in urea and creatinine levels in diabetic rats. Study Method: Twenty-five Sprague Dawley rats were divided into 5 groups, the first group was a healthy control group, group 2 was a diabetic control group, while groups 3,4, and 5 were diabetes rats with ethanol extract of Tithonia diversifolia leaves at a dose, 25; 50; and 100 mg/kg BW for 28 days. Induction of diabetes with nicotinamide (230 mg/kg BW) and streptozotocin (65 mg/kg BW). The rat blood serum was taken and the urea-creatinine was examined by the enzymatic method. Data analysis used the ANOVA test and LSD test. Result: The urea levels in groups 1,2,3,4 and 5 were respectively 28.8±1.3; 75.0±14.3; 59.2±17.5; 51.0±15.7; and 35.0±3.6 mg/dL. Furthermore, the creatinine levels in groups 1,2,3,4 and 5 were respectively 0,9675 ± 0,033; 1,1625 ± 0,057; 1,1325 ± 0,144; 1,025 ± 0,075; dan 0,9875 ± 0,106 mg/dL. The statistical test showed that there were differences in urea-creatinine levels in group 5 with groups 2 (p=0.000; p=0.016), while there was no difference between groups 1 (p=0.487;p=0.762). Conclusion: Tithonia diversifolia leaf extract at a dose of 100 mg/kg BW can prevent the increase in urea-creatinine levels in diabetic rats. |
| Kata kunci | Daun kembang bulan, Tithonia diversifolia, ureum, kreatinin, stress oxidative |
| Pembimbing 1 | DR. Yulia Fauziyah, S.Kep, M.Sc |
| Pembimbing 2 | dr. Hajid Rahmadianto M., M.Sc, Sp.U |
| Pembimbing 3 | |
| Tahun | 2021 |
| Jumlah Halaman | 15 |
| Tgl. Entri | 2021-01-21 13:18:38.608778 |