Artikel Ilmiah : I1C016061 a.n. RIZA TRI ANGGORO
| NIM | I1C016061 |
|---|---|
| Namamhs | RIZA TRI ANGGORO |
| Judul Artikel | ANALISIS LC-MS EKSTRAK DAUN SLATRI (Calophyllum soulattri Burm. f) ASAL BANYUMAS DAN AKTIVITAS ANTIJAMUR TERHADAP C. albicans ATCC 10231 |
| Abstrak (Bhs. Indonesia) | Latar Belakang : Candida albicans adalah flora normal dalam tubuh manusia yang sering menimbulkan infeksi oleh jamur yang disebut kandidiasis. Terapi pada kandidiasis seperti nistatin mengalami resisten untuk C. albicans. Salah satu alternatif obat antijamur yang resisten menggunakan daun C. soulattri memiliki potensi sebagai agen antijamur. Tujuan penelitian ini untuk mengetahui senyawa metabolit sekunder yang terkandung dan aktivitas antijamur terhadap C. albicans dari fraksi n-heksan, fraksi etil asetat, dan ekstrak metanol daun C. soulattri asal Banyumas. Metodologi : Ekstrak metanol daun C. soulattri dipartisi menjadi fraksi n-heksan dan fraksi etil asetat. Fraksi n-heksan, fraksi etil asetat, dan ekstrak metanol daun C. soulattri dengan konsentrasi kadar 50% diuji LC-MS/MS dan uji aktivitas antijamur. Hasil Penelitian : Fraksi dan ekstrak daun C. soulattri mengandung senyawa metabolit sekunder golongan senyawa flavonoid, triterpenoid dan fenol. Penelitian menunjukkan bahwa fraksi n-heksan, fraksi etil asetat, dan ekstrak metanol daun slatri asal Banyumas aktif sebagai antijamur terhadap C. albicans. Kesimpulan : Fraksi n-heksan mengandung senyawa amentoflavon, asam porikoat D, asam porikoat F serta dua senyawa candidate mass C45H84O14 dan C54H78O10. Fraksi etil asetat mengandung senyawa amentoflavon, gomisin K2, kaempferol-7-O-α-L-rhamnoside, asam porikoat D serta satu senyawa candidate mass C23H30O5. Ekstrak metanol mengandung senyawa amentoflavon, asam porikoat D serta tiga senyawa candidate mass C45H84O14, C54H78O10 dan C26H48O14. Fraksi n-heksan, fraksi etil asetat, dan ekstrak metanol daun C. soulattri memiliki aktivitas antijamur terhadap C. albicans ATCC 10231 dengan diameter hambat 5,23 mm (sedang), 5,08 mm (sedang), dan 1,35 mm (lemah) pada konsentrasi 50 %. |
| Abtrak (Bhs. Inggris) | Background: Candida albicans is a normal flora in the human body that often causes infection by a fungus called candidiasis. Treatment of candidiasis such as nystatin is resistant to C. albicans. One of the alternative resistant antifungal drugs using C. soulattri leaves has potential as an antifungal agent. The purpose of this study was to determine the secondary metabolite compounds contained and antifungal activity against C. albicans from the n-hexane fraction, ethyl acetate fraction, and methanol extract of C. soulattri leaves from Banyumas. Methodology: The methanol extract of C. soulattri leaves was partitioned into n-hexane and ethyl acetate fractions. The n-hexane fraction, ethyl acetate fraction, and methanol extract of C. soulattri leaves with a concentration of 50% were tested by LC-MS / MS and antifungal activity. Research Results: The leaf fraction and extract of C. soulattri contains secondary metabolites, flavonoids, triterpenoids and phenols. The study showed that the n-hexane fraction, ethyl acetate fraction, and the methanol extract of slatri leaves from Banyumas were active as an antifungal against C. albicans. Conclusion: The n-hexane fraction of slatri leaves from Banyumas contains amentoflavone, poricic acid D, poricic acid F and two candidate compounds mass C45H84O14 and C54H78O10. The ethyl acetate fraction contains amentoflavone, gomycin K2, kaempferol-7-O-α-L-rhamnoside, acid porikoat D and one candidate mass C23H30O5. The methanol extract contains amentoflavone, poricic acid D and three candidate mass compounds C45H84O14, C54H78O10 and C26H48O14. The n-hexane fraction, ethyl acetate fraction, and methanol extract of C. soulattri leaves had antifungal activity against C. albicans ATCC 10231 with an inhibitory diameter of 5.23 mm (moderate), 5.08 mm (moderate), and 1.35 mm ( weak) at a concentration of 50%. |
| Kata kunci | Ekstrak metanol, LC-MS/MS, C. soulattri Burm. f, C. albicans. |
| Pembimbing 1 | Dr. apt. Sri Sutji Susilowati, M.Si. |
| Pembimbing 2 | Dr. Muhamad Salman Fareza, S.Si., M.Si |
| Pembimbing 3 | - |
| Tahun | 2021 |
| Jumlah Halaman | 10 |
| Tgl. Entri | 2021-01-07 01:56:58.299689 |