| NIM | B1K014006 |
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| Namamhs | NADYATUL HANIFAH |
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| Judul Artikel | ENTEROBACTERIACEAE CONTAMINATION IN ORGANIC LETTUCE (Lactuca sativa) SOLD AT SUPERMARKET IN PURWOKERTO, BANYUMAS REGENCY |
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| Abstrak (Bhs. Indonesia) | Many outbreaks around the world involve the microbial contamination on the green leaves. Organic vegetables were more likely to be exposed to microbial contamination due to the application of manure fertilizer. The issue made many people worry when consuming organic vegetables, especially veggies that were consuming fresh like lettuce (Lactuca sativa). Microbial contamination of vegetables usually comes from bacteria of the family Enterobacteriaceae. Enterobacteriaceae is widely distributed and commonly found in the environment, consist of non-pathogenic and pathogenic bacteria. The objectives of this research were to know the Enterobacteriaceae population on organic lettuce sold at Supermarket in Purwokerto and to know the most and the highest Enterobacteriaceae isolates contaminate organic lettuce. This study was carried out by a survey method, and samples were taken from three brands sold at Supermarket around Purwokerto. The leaves samples were rinsed with tap water and diluted, then cultured in MacConkey Agar medium. The enumeration of the bacterial population used Standard Plate Count (SPC) method. Nineteen isolates of Gram-negative bacteria were obtained from organic lettuce leaves (Lactuca sativa). The Result of identification found 19 isolates belong to genera Enterobacter, Klebsiella, Serratia, Escherichia, and Pseudomonas. The Enterobacteriaceae population of brand A contained the lowest bacterial density of 4,70.104 CFU.g-1. In comparison, samples from brand B and C were 6,57.104 CFU.g-1 and 5,50.104 CFU.g-1, respectively, which were not following ICMSF recommendations for consumption, namely less than 4 log CFU.g-1. All the identified genera were present in all three samples brand. The highest Enterobacteriaceae contaminated organic lettuce were Enterobacter, dominates by 66.3%, followed by Klebsiella, Serratia and the least population was Escherichia, and the average number of bacterial population were 4,49.104 CFU.g-1, 5,78.103 CFU.g-1, 3,57.103 CFU.g-1, 1,64.103 CFU.g-1, respectively. The decontamination process could be carried out with the correct preparation process, such as chlorinated water or mild hot water. |
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| Abtrak (Bhs. Inggris) | Many outbreaks around the world involve the microbial contamination on the green leaves. Organic vegetables were more likely to be exposed to microbial contamination due to the application of manure fertilizer. The issue made many people worry when consuming organic vegetables, especially veggies that were consuming fresh like lettuce (Lactuca sativa). Microbial contamination of vegetables usually comes from bacteria of the family Enterobacteriaceae. Enterobacteriaceae is widely distributed and commonly found in the environment, consist of non-pathogenic and pathogenic bacteria. The objectives of this research were to know the Enterobacteriaceae population on organic lettuce sold at Supermarket in Purwokerto and to know the most and the highest Enterobacteriaceae isolates contaminate organic lettuce. This study was carried out by a survey method, and samples were taken from three brands sold at Supermarket around Purwokerto. The leaves samples were rinsed with tap water and diluted, then cultured in MacConkey Agar medium. The enumeration of the bacterial population used Standard Plate Count (SPC) method. Nineteen isolates of Gram-negative bacteria were obtained from organic lettuce leaves (Lactuca sativa). The Result of identification found 19 isolates belong to genera Enterobacter, Klebsiella, Serratia, Escherichia, and Pseudomonas. The Enterobacteriaceae population of brand A contained the lowest bacterial density of 4,70.104 CFU.g-1. In comparison, samples from brand B and C were 6,57.104 CFU.g-1 and 5,50.104 CFU.g-1, respectively, which were not following ICMSF recommendations for consumption, namely less than 4 log CFU.g-1. All the identified genera were present in all three samples brand. The highest Enterobacteriaceae contaminated organic lettuce were Enterobacter, dominates by 66.3%, followed by Klebsiella, Serratia and the least population was Escherichia, and the average number of bacterial population were 4,49.104 CFU.g-1, 5,78.103 CFU.g-1, 3,57.103 CFU.g-1, 1,64.103 CFU.g-1, respectively. The decontamination process could be carried out with the correct preparation process, such as chlorinated water or mild hot water. |
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| Kata kunci | Enterobacteriaceae, Organic lettuce, Organic vegetable |
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| Pembimbing 1 | Dr. Oedjijono, M.Sc |
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| Pembimbing 2 | Dra. Dini Ryandini, M.Si |
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| Pembimbing 3 | |
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| Tahun | 2020 |
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| Jumlah Halaman | 7 |
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| Tgl. Entri | 2020-08-21 11:02:30.51391 |
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